Fluorometric determination of pantothenic acid in human urine by isocratic reversed-phase ion-pair high-performance liquid chromatography with post-column derivatization

Takahashi, Kei; Fukuwatari, Tsutomu; Shibata, Katsumi

doi:10.1016/j.jchromb.2009.05.056

Cited by 17 publications

(6 citation statements)

References 20 publications

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“…Pantothenic acid, also known as vitamin B5, is one of the 13 essential vitamins in humans. It consists of pantoic acid and β -alanine, which is an acyl transporter and participates in the metabolism process, regulating energy and fat metabolism [ 34 ]. Acetyl-CoA, an acetylated form of CoA, is a precursor of substances such as fatty acids and ketone bodies and plays a pivotal role in the energy metabolism in vivo [ 35 ], whereas CoA can form acyl carrier protein and further produce R-4-'phospho-pantothenate which is associated with pantothenic acid.…”

Section: Discussionmentioning

confidence: 99%

Study of the Treatment Effects of Compound Tufuling Granules in Hyperuricemic Rats Using Serum Metabolomics

Zhang

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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The study aimed to investigate the mechanism of the effect of Compound Tufuling Granules (CTG) to lower the serum uric acid level in a rat model of hyperuricemia. The rat model was established by administering hypoxanthine through oral gavage and potassium oxonate through intraperitoneal injection. Rats were divided into the normal group, model group, CTG group, and allopurinol group. Serum uric acid, creatinine, urea nitrogen, and inflammatory cytokine levels were determined in each group. In the model group, ultrahigh performance liquid chromatography-mass spectrometry was used to analyze the metabolic profiles and delineate the action mechanism of CTG; in addition, the orthogonal projection method was used to perform latent structure-discrimination analysis to screen the related metabolites. The results indicated significant differences in the metabolic profiles between the model and normal groups. A total of seven related metabolites were identified through screening in the model group, mainly related to the pathways of bile secretion, pyrimidine, purine, and phenylalanine metabolism, pantothenate and CoA biosynthesis, and pentose and glucuronate interconversions; these related pathways were reversed in the CTG group. In the metabolic networks, uracil and acetyl-coenzyme A were the nodal molecules. In addition, the test results of the evaluation of serum biochemical and inflammatory factors confirmed that CTG had significant effect in reducing the levels of serum uric acid and protecting renal function. These results confirmed that CTG primarily regulated the recruitment of nodal molecules to achieve anti-inflammatory effects, reduced uric acid level, and renal protection.

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Section: Discussionmentioning

confidence: 99%

Study of the Treatment Effects of Compound Tufuling Granules in Hyperuricemic Rats Using Serum Metabolomics

Zhang

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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“…Urinary content of 2-Py, 4-Py and MNA was determined by the HPLC method (18,23). For analysis of pantothenic acid, a urine sample was injected directly into a HPLC (24).…”

Section: Methodsmentioning

confidence: 99%

Urinary Excretion Levels of Water-Soluble Vitamins in Pregnant and Lactating Women in Japan

Shibata

Fukuwatari

Sasaki

et al. 2013

J Nutr Sci Vitaminol

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Dietary habits of pregnant and lactating women have a critical impact on the health of future infants. For this reason, the Japanese Dietary Reference Intakes regarding the amounts of addition of water-soluble vitamins have been set (1). The additional intake amounts for pregnant and lactating women were calculated based on data concerning the changes in the metabolic characteristics of vitamins in pregnant women and the loss of vitamins as a result of lactation. However, there is little information about whether nutritional statuses of water-soluble-vitamins in Japanese pregnant and lactating women are good or not. Therefore, we examined the nutritional status of pregnant and lactating women and speculated about the validity of the additional amount of some water-soluble vitamins in the dietary reference intakes for pregnant and lactating women.A potential approach for determining the validity for the additional amounts is based on the observation that a water-soluble vitamin or its catabolite(s) can be detected in the urine (2-11). Using this approach, the urinary excretion of a water-soluble vitamin or its catabolite(s) occurs when the dietary intake exceeds an individual necessary amount. MATERIALS AND METHODSSubjects. Japanese pregnant (16-40 wk gestation) or lactating women (0-11 mo postpartum) were recruited from a parenting circle in The University of Shiga Prefecture between April 2010 and February 2012. Japanese married non-pregnant, non-lactating women also were recruited as controls through the parenting circle to match the mean ages and heights of the pregnant and lactating women. The purpose and protocol of this study was explained to all participants before joining the study, and written informed consent was obtained from each participant. We excluded participants diagnosed with a cold or influenza, and those who had taken multi-vitamin supplements at least once during the previous month. In addition, we excluded participants whose 24-h urine collection was considered incomplete: a collection time outside the 22-26-h range, urine volume ,250 mL, or extremely low or high energy intake (,500 or .4,000 kcal/d) (12). Twenty-four of the 2nd trimester pregnant (16-27 wk gestation) and 32 of the Bunkyo-ku, Tokyo 113-0033, Japan (Received December 17, 2012) Summary Recent studies have shown that the urinary excretion levels of water-soluble vitamins can be used as biomarkers for the nutritional status of these vitamins. To determine changes in the urinary excretion levels of water-soluble vitamins during pregnant and lactating stages, we surveyed and compared levels of nine water-soluble vitamins in control (non-pregnant and non-lactating women), pregnant and lactating women. Control women (n537), women in the 2nd (16-27 wk, n524) and 3rd trimester of pregnancy (over 28 wk, n532), and early-(0-5 mo, n554) and late-stage lactating (6-11 mo, n549) women took part in the survey. The mean age of subjects was ~30 y, and mean height was ~160 cm. A single 24-h urine sample was collected 1 d after the comple...

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“…Pantothenic acid . Pantothenic acid in urine was determined by HPLC ( 25 ) . To digest the bound pantothenic acid including coenzyme A and phosphopantetheine in tissue and plasma to free form, the homogenate or blood was incubated at 37°C for 24 h. Pantothenic acid in the plasma and tissue was determined by the microbioassay method using Lactobacillus plantarum ATCC 8014 ( 26 ) .…”

Section: Methodsmentioning

confidence: 99%

Different variations of tissue B-group vitamin concentrations in short- and long-term starved rats

et al. 2011

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Prolonged starvation changes energy metabolism; therefore, the metabolic response to starvation is divided into three phases according to changes in glucose, lipid and protein utilisation. B-group vitamins are involved in energy metabolism via metabolism of carbohydrates, fatty acids and amino acids. To determine how changes in energy metabolism alter B-group vitamin concentrations during starvation, we measured the concentration of eight kinds of B-group vitamins daily in rat blood, urine and in nine tissues including cerebrum, heart, lung, stomach, kidney, liver, spleen, testis and skeletal muscle during 8 d of starvation. Vitamin B 1 , vitamin B 6 , pantothenic acid, folate and biotin concentrations in the blood reduced after 6 or 8 d of starvation, and other vitamins did not change. Urinary excretion was decreased during starvation for all B-group vitamins except pantothenic acid and biotin. Less variation in B-group vitamin concentrations was found in the cerebrum and spleen. Concentrations of vitamin B 1 , vitamin B 6 , nicotinamide and pantothenic acid increased in the liver. The skeletal muscle and stomach showed reduced concentrations of five vitamins including vitamin B 1 , vitamin B 2 , vitamin B 6 , pantothenic acid and folate. Concentrations of two or three vitamins decreased in the kidney, testis and heart, and these changes showed different patterns in each tissue and for each vitamin. The concentration of pantothenic acid rapidly decreased in the heart, stomach, kidney and testis, whereas concentrations of nicotinamide were stable in all tissues except the liver. Different variations in B-group vitamin concentrations in the tissues of starved rats were found. The present findings will lead to a suitable supplementation of vitamins for the prevention of the re-feeding syndrome.Key words: Starvation: Fasting: Energy metabolism Starvation produces a series of metabolic changes that lead to a reduction in body weight, alterations in body composition and metabolic gene expression (1,2) . In mammals and birds, three distinct levels of energy depletion have been established (3 -10) . The first phase (phase 1) is a rapid period of adaptation marked by an increase in mobilisation of fat stores and a lowering in protein utilisation. During the second phase (phase 2), which is a long period of thrift, most of the energy expenditure is derived from fats, and then fat stores are progressively exhausted, while body proteins are efficiently spared. The third phase (phase 3) is characterised by an increase in protein utilisation. In humans, the negative energy balance resulting from starvation can arise due to disease, eating or psychological disorders, or hunger strikes. Starvation and consequent re-feeding syndrome can lead to electrolyte disorders, especially hypophosphataemia, along with neurological, pulmonary, cardiac, neuromuscular and haematological complications (11) . To avoid the re-feeding syndrome, an additional load of vitamins has been suggested to correct the vitamin deficiencies (11) . ...

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Fluorometric determination of pantothenic acid in human urine by isocratic reversed-phase ion-pair high-performance liquid chromatography with post-column derivatization

Cited by 17 publications

References 20 publications

Study of the Treatment Effects of Compound Tufuling Granules in Hyperuricemic Rats Using Serum Metabolomics

Study of the Treatment Effects of Compound Tufuling Granules in Hyperuricemic Rats Using Serum Metabolomics

Urinary Excretion Levels of Water-Soluble Vitamins in Pregnant and Lactating Women in Japan

Different variations of tissue B-group vitamin concentrations in short- and long-term starved rats

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