A comparison of inflammatory exudates released from myiasis wounds on sheep bred for resistance or susceptibility to Lucilia cuprina

O'Meara, T.J.; Nesa, M.; Seaton, D.S.; Sandeman, R.M.

doi:10.1016/0304-4017(94)00676-4

Cited by 14 publications

(16 citation statements)

References 36 publications

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“…The contribution of the skin barrier, bacterial communities and the sheep local skin inflammatory, innate and adaptive immune responses to FR susceptibility are less well studied and understood. Divergent serological responses against Pseudomonas aeruginosa [8] and immune-inflammatory responses [1,9,10] have been documented in FR and fly strike resistant (RES) and susceptible (SUS) animals. Ovine defence mechanisms associated with skin IgE+ cells were found to play an important role in resistance to fleece rot [9].…”

Section: Introductionmentioning

confidence: 99%

A genomics-informed, SNP association study reveals FBLN1 and FABP4 as contributing to resistance to fleece rot in Australian Merino sheep

Smith

Ingham

et al. 2010

BMC Vet Res

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BackgroundFleece rot (FR) and body-strike of Merino sheep by the sheep blowfly Lucilia cuprina are major problems for the Australian wool industry, causing significant losses as a result of increased management costs coupled with reduced wool productivity and quality. In addition to direct effects on fleece quality, fleece rot is a major predisposing factor to blowfly strike on the body of sheep. In order to investigate the genetic drivers of resistance to fleece rot, we constructed a combined ovine-bovine cDNA microarray of almost 12,000 probes including 6,125 skin expressed sequence tags and 5,760 anonymous clones obtained from skin subtracted libraries derived from fleece rot resistant and susceptible animals. This microarray platform was used to profile the gene expression changes between skin samples of six resistant and six susceptible animals taken immediately before, during and after FR induction. Mixed-model equations were employed to normalize the data and 155 genes were found to be differentially expressed (DE). Ten DE genes were selected for validation using real-time PCR on independent skin samples. The genomic regions of a further 5 DE genes were surveyed to identify single nucleotide polymorphisms (SNP) that were genotyped across three populations for their associations with fleece rot resistance.ResultsThe majority of the DE genes originated from the fleece rot subtracted libraries and over-representing gene ontology terms included defense response to bacterium and epidermis development, indicating a role of these processes in modulating the sheep's response to fleece rot. We focused on genes that contribute to the physical barrier function of skin, including keratins, collagens, fibulin and lipid proteins, to identify SNPs that were associated to fleece rot scores.ConclusionsWe identified FBLN1 (fibulin) and FABP4 (fatty acid binding protein 4) as key factors in sheep's resistance to fleece rot. Validation of these markers in other populations could lead to vital tests for marker assisted selection that will ultimately increase the natural fleece rot resistance of Merino sheep.

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Section: Introductionmentioning

confidence: 99%

A genomics-informed, SNP association study reveals FBLN1 and FABP4 as contributing to resistance to fleece rot in Australian Merino sheep

Smith

Ingham

et al. 2010

BMC Vet Res

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“…These R vs. S sheep were also monitored for a range of molecules present in exudates collected at varying times from flystrike wounds. Significant differences were detected in the exudate compositions for Complement C3, a fragment of IgG, a1-antitrypsin and for a range of unidentified peptides, and it was suggested at the time that the more rapid exudation of acute-phase and serum proteins at infection sites on R sheep may allow the inhibition of the establishment of fleece rot bacteria or L. cuprina larvae under natural challenge (41). The Trangie flock may therefore harbour important information on the innate response to challenge infection with the larvae in the field.…”

Section: Inflammation Vs Immunitymentioning

confidence: 97%

“…These humoral responses have been shown to be directed against E/S products of the larvae (42,43) and the salivary antigens (37) following natural infection, while the response has been characterized by the production of IgG (36,41) with subsequent analysis identifying a number of isotypes including IgG1, IgG2, IgA and IgM (44) and IgE (45) with the primary isotype being identified as IgG1 (42).…”

Section: Sheep Blowfly Infectionsmentioning

confidence: 98%

“…The humoral immune responses to infection was first reported back in the early 1980s (36), and several groups have further investigated the nature of both the systemic and local humoral response during infection (28,(37)(38)(39)(40)(41). These humoral responses have been shown to be directed against E/S products of the larvae (42,43) and the salivary antigens (37) following natural infection, while the response has been characterized by the production of IgG (36,41) with subsequent analysis identifying a number of isotypes including IgG1, IgG2, IgA and IgM (44) and IgE (45) with the primary isotype being identified as IgG1 (42).…”

Section: Sheep Blowfly Infectionsmentioning

confidence: 99%

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The immunobiology of myiasis infections – whatever happened to vaccination?

Sandeman

Bowles

Colwell³

2014

Parasite Immunology

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The current state of myiasis vaccine technologies are reviewed mainly in the primary research genera of Lucilia and Hypoderma. The importance of myiasis flies as primary causes of morbidity and mortality in agricultural species and man has not diminished despite the existence of good control strategies. However, the development of vaccines against myiasis infections has been relatively quiescent for more than 10 years despite the rapid development of genomic and proteomic analysis and of skills in data interpretation. The value of vaccine research in an era of chemical primacy is analysed. In fact, recent findings of drug resistance and the impact of animal welfare concerns should mean a renewed interest in alternative controls. The reasons that this has not been true to date are explored and new possibilities discussed.

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“…The mechanisms responsible for differences in resistance expressed by the R and S animals remain unclear. R and S animals differ in antibody titres to a Pseudomonas aeruginosa vaccine (Chin & Watts 1991), intradermal inflammation following administration of L. cuprina excretory/ secretory products , plasma leakage and cellular recruitment following administration of inflammatory mediators (Colditz et al , 1994 and inflammatory exudate production upon challenge by L. cuprina (O'Meara et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Antibody responses to Lucilia cuprina in sheep selected for resistance or susceptibility to L. cuprina

O'Meara

Nesa

Sandeman

1997

Parasite Immunology

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Sheep bred for resistance (R) or susceptibility (S) to fleece rot and myiasis (blowfly strike) have been shown to differ in inflammatory response to intradermal administration of blowfly (Lucilia cuprina) antigens and artificial challenge. The current paper describes analysis of antibody responses to L. cuprina antigens in the R and S animals. Serum antibody titres and specificities to larval antigens were examined and the specificity of wound exudate antibodies was also investigated in animals artificially challenged with L. cuprina. Titres of L. cuprina specific serum IgA, IgM, IgG2 and IgG1 were measured by ELISA, while specificities were examined on two-dimensional immunoblots of larval homogenates. Exposure to L. cuprina stimulated the production of specific antibody in both R and S animals, however antibody titres did not differ between the R and S animals. There was large variation in antibody specificity between individual animals and some L. cuprina proteins appear to be more frequently recognized by sera from either resistant or susceptible animals, however the recognition of a specific protein could not be solely attributed to the resistance status of the animal. It appears that resistance in these animals may be independent of serum antibody and is likely to be an innate response. Despite high levels of IgG in wound exudates, this antibody recognized few antigens in comparison with serum from the same animal, suggesting that exudate contains little functional antibody in comparison to serum.

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A comparison of inflammatory exudates released from myiasis wounds on sheep bred for resistance or susceptibility to Lucilia cuprina

Cited by 14 publications

References 36 publications

A genomics-informed, SNP association study reveals FBLN1 and FABP4 as contributing to resistance to fleece rot in Australian Merino sheep

A genomics-informed, SNP association study reveals FBLN1 and FABP4 as contributing to resistance to fleece rot in Australian Merino sheep

The immunobiology of myiasis infections – whatever happened to vaccination?

Antibody responses to Lucilia cuprina in sheep selected for resistance or susceptibility to L. cuprina

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