A comparison of Api 20A vs MALDI-TOF MS for routine identification of clinically significant anaerobic bacterial strains to the species level

High-throughput discrimination of bacteria isolated fromAstacus astacusandA. leptodactylus

“…When comparing the performance of MALDI-TOF MS with conventional and API systems for clinical isolates of human material, the percentage of correct identifications is significantly higher than in this work, mainly due to the customized databases (Bizzini et al, 2010;Dupont et al, 2010;Risch et al, 2010;Martiny et al, 2011;Saffert et al, 2011;El-Bouri et al, 2012;Nagy et al, 2012;Campos Braga et al, 2013;Kierzkowska et al, 2013). For example, 97.2% of isolates had identical genus identification by both methods, while 79.9% yielded exactly the same species identification in the work of van Veen et al (2010), and conventional methods also resulted in fewer species identification (83.1% versus 92% MALDI-TOF MS).…”

“…identification (A. hydrophila group 1 (API 20E) versus A. bestiarum (MALDI-TOF MS)) can be attributed to close relatedness of the two species, which according to Martino et al (2011) belong to the same phenogroup, described as the A. hydrophila complex. The current taxonomic database of the MALDI-TOF MS Biotyper system recognizes species that are currently of different taxonomic status and have not been updated in the Apiweb system (Kierzkowska et al, 2013). The genus Aeromonas comprises 21 validly proposed species, and some of them are phenotypically very similar.…”

“…Generally, diagnostic methods based on phenotypic analysis are less frequently used, and molecular methods dominate over traditional techniques, the golden standard being the 16S rRNA gene sequencing. Nevertheless, the high cost of this assessment makes this technique impossible 04p9 to use in routine microbiology diagnostics (Kierzkowska et al, 2013) and the next step is the mass spectrometry-assisted identification. MALDI-TOF MS has demonstrated to be a competent bacterial typing tool that extends phenotypic and genotypic approaches, allowing a more ample classification of bacterial strains (Böhme et al, 2013).…”

Specific Character of Anaerobic Bacterial Infections in Patients Treated in Transplantation Wards at One of the Clinical Hospitals in Warsaw

“…The results of microbiological tests become available after the empirical treatment has already been initiated, often with a good therapeutic effect [5]. Samples obtained from the ward II in our study observed Gram-positive bacteria: Peptostreptococcus spp.…”

MALDI-TOF Mass Spectrometry for Microorganism Identification

“…MALDI-TOF MS has now been demonstrated to be more accurate than phenotypic identification methods (e.g. RapID 32A) (Barba et al, 2014;Chean et al, 2014;Kierzkowska, Majewska, Kuthan, Sawicka-Grzelak, & Mlynarczyk, 2013).…”