2020
DOI: 10.1002/fsn3.1841
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A comparative UPLC‐Q‐Orbitrap‐MS untargeted metabolomics investigation of different parts of Clausena lansium (Lour.) Skeels

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Cited by 21 publications
(7 citation statements)
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“…Heat map and KEGG annotation showed a significant enrichment of the “Flavone and flavonol synthesis” and “Isoquinoline alkaloid biosynthesis” pathway. This investigation could provide a foundation for the isolation and identification of new constituents from Clausena lansium and further clarified the biosynthetic pathway of differential metabolites among various tissues of Clausena lansium , which can be used for reference by other studies of CMM [ 79 ].…”
Section: The Application Of Plant Metabolomics In Quality Evaluation ...mentioning
confidence: 99%
“…Heat map and KEGG annotation showed a significant enrichment of the “Flavone and flavonol synthesis” and “Isoquinoline alkaloid biosynthesis” pathway. This investigation could provide a foundation for the isolation and identification of new constituents from Clausena lansium and further clarified the biosynthetic pathway of differential metabolites among various tissues of Clausena lansium , which can be used for reference by other studies of CMM [ 79 ].…”
Section: The Application Of Plant Metabolomics In Quality Evaluation ...mentioning
confidence: 99%
“…Secondly, the extraction was acquired by centrifugation for 10 min at 12,000 rpm (microcentrifuge). Finally, 300 μL supernatant was ltered through a 0.22-µm membrane (Tianjin JinTeng Instrument Company, Tianjin, China) to obtain samples for LC-MS. To monitor deviations caused by the analytical instrument itself, a quality control (QC) sample was also analyzed by mixing 20 µL of each individual sample.The LC-MS analysis was conducted based on our previous protocol [25] and six replicates of each sample were analyzed. To investigate metabolic pathways, the identi ed metabolites were aligned to biochemical pathways provided by KEGG (http://www.kegg.jp/pathway).To investigate metabolic pathways, the identi ed metabolites were aligned to biochemical pathways provided by KEGG (http://www.kegg.jp/pathway).…”
Section: Metabolite Extraction and Untargeted Lc-ms Analysismentioning
confidence: 99%
“…Non-targeted metabolomics, which uses detectors such as time-of-flight (TOF), Fourier-transform ion cyclotron resonance (FT-ICR), and Orbitrap, can identify thousands of metabolites and is biased toward high-resolving power, detecting as many metabolites as possible without quantification [ 40 ]. Targeted metabolomics, in contrast, uses a triple quadrupole (QQQ) detector to target and quantify a particular group of molecules in order to obtain highly sensitive detection and quantification [ 41 , 42 , 43 , 44 ].…”
Section: Food Waste Managementmentioning
confidence: 99%