Integrated transcriptomic and metabolomic analyses reveal key genes controlling flavonoid biosynthesis in Citrus grandis ‘Tomentosa’ fruits

Transcriptomic and targeted metabolome analyses revealed the regulatory mechanisms of the synthesis of bioactive compounds in Citrus grandis ‘tomentosa’

“…An analysis of the appearance and secondary metabolites of the three main varieties of C. grandis revealed that the trichomes and flavonoids of the three varieties were different ( Fan et al, 2019 ; Tian, Fan & Zeng, 2021 ). Fan et al (2023) further screened 25 genes and 16 transcription regulatory factors related to flavonoid biosynthesis using metabolomics and transcriptomics, which may be involved in the biosynthesis of flavonoids in C. grandis ‘tomentosa’ fruit. Further analysis of the role of Jumonji C (JMJC) domain family members in the regulation of the external morphology and internal quality of C. grandis shows that some CgJMJC genes show different expression patterns during the fruit development of three varieties, indicating that they may play a role in the formation of unique phenotypes ( Tian, Fan & Zeng, 2021 ); however, their specific functions and whether they regulate the synthesis of secondary metabolites still need further study.…”

“… Zheng et al (2023) constructed a regulatory pathway for C. grandis flavonoids and identified a transcription factor CgMYB108, which can negatively regulate the flavonoid synthesis pathway by interacting with the promoters of PAL (phenylalanine ammonia lyase) and FNS (flavone synthesis). However, although there have been studies on the synthesis and regulation mechanisms of some active ingredients in citrus red, the main focus has been on the synthesis of flavonoids, and there have been no research reports on the synthesis and regulation of volatile oils ( Fan et al., 2023 ; Zheng et al, 2023 ). Therefore, the aim of the present study was to apply transcriptomics and targeted metabolomics techniques to detect differences in the contents and gene expressions of five flavonoids and volatile oils in different tissues of C. grandis , and further analyze the synthesis and regulation mechanisms of bioactive compounds.…”

Integrated Untargeted Metabolome, Full-Length Sequencing and Transcriptome Analyses Reveal the Mechanism of Flavonoid Biosynthesis in Blueberry (Vaccinium spp.) Fruit

“…Phenolic acids are mainly represented by chlorogenic acid, which is generated by the esterification of caffeic acid with a quinic acid molecule [8,9]. The biosynthesis of flavonoids has been explored in various plants such as Arabidopsis thaliana [10], Ananas comosus [11], Vitis vinifera [12], Citrus grandis [13], and Solanum tuberosum [14]. The structural genes involved in the biosynthesis pathways of flavonoids can be divided into two groups: early biosynthesis genes (EBGs) and late biosynthesis genes (LBGs).…”