A comparative study of phycobilliprotein production in two strains of Pseudanabaena isolated from Arctic and tropical regions in relation to different light wavelengths and photoperiods

Khan, Zoya; Maznah, W. O. Wan; Merican, M.S.M. Faradina; Convey, Peter; Najimudin, Nazalan; Alias, Siti Aisyah

doi:10.1016/j.polar.2018.10.002

Cited by 20 publications

(12 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The presence of phycobiliprotein was verified from supernatant analysis by spectrophotometry (Shimadzu UV mini-1240) at the specific wavelengths of phycoerythrin (PE) 562 nm, phycocyanin (PC) 615 nm and allophycocyanin (APC) 652 nm and, according to Eqs. 1-3, each PBP concentration (mg•mL −1 ) was calculated (Johnson et al 2014;Khazi et al 2018;Khan et al 2019),…”

Section: Determination Of Phycobiliprotein Concentrationmentioning

confidence: 99%

Effect of glucose and sodium nitrate on the cultivation of Nostoc sp. PCC 7423 and production of phycobiliproteins

Cottas

Teixeira

Cunha

et al. 2021

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

This study aimed to investigate the influence of glucose, sodium nitrate and initial inoculum ratio in order to obtain cell biomass and phycobiliproteins from the Nostoc sp. PCC 7423. The optimum supplementation condition corresponded to the simultaneous addition of glucose 2 g•L −1 and sodium nitrate 5 mM, capable of increasing the concentration of biomass by about threefold (reaching 0.54 g•L −1 ) and the PC concentration by 2.3-fold (reaching 133.03 mg•g −1 ), compared to the basal medium BG11 0 (control). The results showed no significant difference with respect of the effect of inoculum ratios, 10% or 20% (p < 0.05), on the phycocyanin production. The condition of simultaneous supplementation of carbon and nitrogen sources is still little studied and the current study indicated that it enabled better results than isolated supplements of glucose and sodium nitrate, which may justify its application to obtain phycobiliproteins.

show abstract

Section: Determination Of Phycobiliprotein Concentrationmentioning

confidence: 99%

Effect of glucose and sodium nitrate on the cultivation of Nostoc sp. PCC 7423 and production of phycobiliproteins

Cottas

Teixeira

Cunha

et al. 2021

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

show abstract

“…(1.23% DW) [ 6 ] and Pseudanabaena sp. (4.17% DW) [ 7 , 8 ], Rhodophyta of Gracilaria gracilis (0.09–0.12% DW) [ 9 , 10 , 11 ], Neopyropia haitanensis (0.25% DW) [ 12 ] and Neopyropia yezoensis (0.80% DW) [ 13 ]. Therefore, Porphyridium is an ideal algal strain for phycoerythrin production.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Production and Fluorescence Characteristics of Phycoerythrin from Three Strains of Porphyridium

Xiang

et al. 2022

Foods

View full text Add to dashboard Cite

Phycoerythrin, a special photosynthetic pigment, is widely used as fluorescent dye and has lots of underlying beneficial effects on health. A marine red microalga Porphyridium is considered as the potential feedstock for phycoerythrin production. However, the phycoerythrin-related properties of Porphyridium have not been systematically evaluated, especially between the species of P. cruentum and P. purpureum. The present study aimed to evaluate the production and fluorescence characteristics of phycoerythrin of three strains of Porphyridium. The results showed that P. purpureum SCS-02 presented the highest biomass, phycoerythrin content and yield were 6.43 g L−1, 9.18% DW and 0.288 g L−1, respectively. There was no significant difference between P. purpureum and P. cruentum in α and β subunits amino acid sequences of phycoerythrin and in fluorescence characteristics. The high gene expression level of the key enzymes in phycoerythrobilin synthesis (porphobilinogen synthase and oxygen-dependent coproporphyrinogen-III oxidase) could be related to the high phycoerythrin content of Porphyridium. Based on systematic evaluation, P. purpureum SCS-02 was selected due to its high biomass and phycoerythrin yield. P. purpureum and P. cruentum were highly similar in the phylogenetic tree, as well as in fluorescence characteristics; therefore, it was speculated that they might be the same Porphyridium species.

show abstract

“…In cyanobacteria, rhodophyta, cryptomonads and cyanell phycobiliproteins are light-harvesting pigment-protein complexes [6] composed of 40-60% of the total soluble protein in cells and found on the outer surface of the thylakoid membrane [7]. Phycobiliproteins are classified into three classes based on their protein structure and absorption spectra as C-phycocyanin (C-PC), allophycocyanin (APC) and phycoerythrin (PE) [8][9][10]. The primary group of phycobiliproteins is C-PC, which is commercially generated in photoautotrophic open raceway pond cultivation of the Arthrospira platensis cyanobacterium [2,11].…”

Section: Introductionmentioning

confidence: 99%

Arthrospira platensis Mutagenesis for Protein and C-Phycocyanin Improvement and Proteomics Approaches

Pan-utai

Iamtham

Roytrakul

et al. 2022

Life

View full text Add to dashboard Cite

Arthrospira (Spirulina) platensis is known for its use as a food supplement, with reported therapeutic properties including antiviral, anti-inflammatory and antioxidant activity. Arthrospira is also an excellent source of proteins and C-phycocyanin. The latter is a light-harvesting pigment-protein complex in cyanobacteria, located on the outer surface of the thylakoid membrane and comprising 40 to 60% of the total soluble protein in cells. Random mutagenesis is a useful tool as a non-genetically modified mutation method that has been widely used to generate mutants of different microorganisms. Exposure of microalgae or cyanobacteria to chemical stimuli affects their growth and many biological processes. Chemicals influence several proteins, including those involved in carbohydrate and energy metabolisms, photosynthesis and stress-related proteins (oxidative stress-reactive oxygen species (ROS) scavenging enzymes). Signal transduction pathways and ion transportation mechanisms are also impacted by chemical treatment, with changes causing the production of numerous biomolecules and stimulation of defence responses. This study compared the protein contents of A. platensis control and after mutagenesis using diethyl sulphate (DES) under various treatment concentrations for effective mutation of A. platensis. Results identified 1152 peptides using proteomics approaches. The proteins were classified into 23 functional categories. Random mutagenesis of A. platensis by DES was found to be highly effective for C-phycocyanin and protein production.

show abstract

A comparative study of phycobilliprotein production in two strains of Pseudanabaena isolated from Arctic and tropical regions in relation to different light wavelengths and photoperiods

Cited by 20 publications

References 32 publications

Effect of glucose and sodium nitrate on the cultivation of Nostoc sp. PCC 7423 and production of phycobiliproteins

Effect of glucose and sodium nitrate on the cultivation of Nostoc sp. PCC 7423 and production of phycobiliproteins

Comparison of Production and Fluorescence Characteristics of Phycoerythrin from Three Strains of Porphyridium

Arthrospira platensis Mutagenesis for Protein and C-Phycocyanin Improvement and Proteomics Approaches

Contact Info

Product

Resources

About