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Phycocyanin (PC) is one of the phycobiliproteins, pigments found in cyanobacteria, which can be used as natural dyes, antioxidants and fluorescent markers. This study aimed to investigate the effect of supplementation of the basal culture medium (BG110) of the cyanobacterium Anabaena variabilis with different sources of organic carbon in concentration of 1 g⸱L-1, evaluating the production of biomass and PC. The best condition obtained was the medium supplemented with glucose that produced 75.36 mg⸱g-1 PC, about 5 times greater than the control with 14.57 mg⸱g-1 PC. All supplemented media also showed higher biomass production compared to the basal medium, again to stand out for the glucose medium, presenting 0.29 g⸱L-1 of dry biomass.

This study aimed to investigate the influence of glucose, sodium nitrate and initial inoculum ratio in order to obtain cell biomass and phycobiliproteins from the Nostoc sp. PCC 7423. The optimum supplementation condition corresponded to the simultaneous addition of glucose 2 g•L −1 and sodium nitrate 5 mM, capable of increasing the concentration of biomass by about threefold (reaching 0.54 g•L −1 ) and the PC concentration by 2.3-fold (reaching 133.03 mg•g −1 ), compared to the basal medium BG11 0 (control). The results showed no significant difference with respect of the effect of inoculum ratios, 10% or 20% (p < 0.05), on the phycocyanin production. The condition of simultaneous supplementation of carbon and nitrogen sources is still little studied and the current study indicated that it enabled better results than isolated supplements of glucose and sodium nitrate, which may justify its application to obtain phycobiliproteins.

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RESUMO-O sistema aquoso bifásico (SAB) é um método de extração líquidolíquido, com aplicações na purificação de biomoléculas, por se tratar de um ambiente biocompatível e não tóxico para estes compostos. Neste trabalho foram utilizados SABs do tipo PEG-sal na proporção 13% polímero e 14% sal, utilizando PEG 1500 e PEG 4000 e os sais fosfato de potássio e citrato de sódio para purificação de ficocianina (FC) de Anabaena variabilis. Dentre os resultados obtidos, observou-se que a FC se particionou para fase topo em todos os sistemas. O SAB formado por PEG 1500fosfato de potássio proporcionou melhores resultados, sendo encontrados coeficiente de partição de 5,44, e pureza de 1,11, que é considerada acima da pureza de grau alimentar.