No abstract

No abstract

No abstract

Phycocyanin (PC) is one of the phycobiliproteins, pigments found in cyanobacteria, which can be used as natural dyes, antioxidants and fluorescent markers. This study aimed to investigate the effect of supplementation of the basal culture medium (BG110) of the cyanobacterium Anabaena variabilis with different sources of organic carbon in concentration of 1 g⸱L-1, evaluating the production of biomass and PC. The best condition obtained was the medium supplemented with glucose that produced 75.36 mg⸱g-1 PC, about 5 times greater than the control with 14.57 mg⸱g-1 PC. All supplemented media also showed higher biomass production compared to the basal medium, again to stand out for the glucose medium, presenting 0.29 g⸱L-1 of dry biomass.

No abstract

Aim: We examined whether ADIPOQ (rs266729 and rs1501299) and NOS3 (rs3918226 and rs1799983) SNPs or the haplotypes formed by them, affect blood pressure (BP) control in 196 patients with adherence to antihypertensive therapy grouped into controlled (BP <140/90 mm Hg) and uncontrolled (BP ≥140/90 mm Hg) hypertension. Materials & methods: The average of the three most recent BP measurements was retrieved from the patients' electronic medical records. Adherence to antihypertensive therapy was evaluated using the Morisky–Green test. Haplotype frequencies were estimated using Haplo.stats. Multiple logistic/linear regression analyses were adjusted for the covariates ethnicity, dyslipidemia, obesity, cardiovascular disease and uric acid. Results: ADIPOQ rs266729 genotypes CG (additive model) and CG+GG (dominant model) were associated with uncontrolled hypertension and CG was associated with higher systolic BP and mean arterial pressure (p < 0.05). ADIPOQ haplotypes ‘GT’ and ‘GG’ were associated with uncontrolled hypertension and ‘GT’ was associated with higher diastolic BP and mean arterial pressure (p < 0.05). Conclusion: ADIPOQ SNPs and haplotypes affect BP control in hypertensive patients undergoing treatment.

This study aimed to investigate the influence of glucose, sodium nitrate and initial inoculum ratio in order to obtain cell biomass and phycobiliproteins from the Nostoc sp. PCC 7423. The optimum supplementation condition corresponded to the simultaneous addition of glucose 2 g•L −1 and sodium nitrate 5 mM, capable of increasing the concentration of biomass by about threefold (reaching 0.54 g•L −1 ) and the PC concentration by 2.3-fold (reaching 133.03 mg•g −1 ), compared to the basal medium BG11 0 (control). The results showed no significant difference with respect of the effect of inoculum ratios, 10% or 20% (p < 0.05), on the phycocyanin production. The condition of simultaneous supplementation of carbon and nitrogen sources is still little studied and the current study indicated that it enabled better results than isolated supplements of glucose and sodium nitrate, which may justify its application to obtain phycobiliproteins.

Purpose: To investigate the association of High Molecular Weight Adiponectin (HMW), HMW/ Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) and HMW/Adiponectin (APN) ratio with hypertension in obese children and adolescents, we evaluated the concentrations of HMW and HMW/APN in the plasma of obese children and adolescents with hypertension.Methods: The 353 pediatric subjects were grouped according to weight and blood pressure.Anthropometric and biochemical parameters were evaluated. Plasma concentrations of APN and HMW were investigated by ELISA. ANOVA was used to compare study groups, and a binary logistic regression analysis was used to verify if HMW, HMW/HOMA-IR, HMW/APN, APN, APN/HOMA-IR, HOMA-IR are predictors of the development of hypertension in children and adolescents. To compare the strength and performance of each biomarker to classify individuals with and without hypertension, the receiver operating characteristic (ROC) curve, area under the curve (AUC) and Youden index (J) were evaluated.Results: Lower HMW and HMW/HOMA-IR levels were associated with hypertension in obese children and adolescents (P<0.05). Hypertension was associated with lowest HMW, HMW/HOMA-IR, APN and APN/HOMA-IR levels, regardless of variables used to adjust the model (P<0.05). However, HOMA-IR or the HMW/APN were not signi cant (P>0.05). In the ROC curve analyses, the HMW and HMW/HOM-IR was more sensitive in predicting hypertension in children and adolescents with obese.Conclusion: Low levels of HMW oligomer and HMW/HOM-IR are associated with hypertension in childhood obesity. Thus, theses biomarkers that could be clinically useful in prognosing hypertension in childhood obesity.