A comparative study of lignocellulosic ethanol productivities by Kluyveromyces marxianus and Saccharomyces cerevisiae

Sandoval-Nuñez, Dania; Arellano‐Plaza, Melchor; Gschaedler, Anne Christine; Arrizón, Javier; Amaya-Delgado, Lorena

doi:10.1007/s10098-017-1470-6

Cited by 24 publications

(13 citation statements)

References 40 publications

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“…However, simple sugars are relatively expensive substrates for economical ethanol production. On the other hand, ethanol can also be produced from agricultural crush (starch and lignocellulose), sugar industry wastes (sugar cane and beet molasses), and dairy industry waste (whey) with a dual purpose of producing energy from cheap sources and alleviation of environmental pollution [ 9 ]. However, the uses of different agricultural wastes require the selection of yeasts that are capable of utilizing substrates derived from hydrolysis of complex carbohydrates.…”

Section: Introductionmentioning

confidence: 99%

Optimization of ethanol production using newly isolated ethanologenic yeasts

Tesfaw

Öner

Assefa

2021

Biochemistry and Biophysics Reports

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Section: Introductionmentioning

confidence: 99%

Optimization of ethanol production using newly isolated ethanologenic yeasts

Tesfaw

Öner

Assefa

2021

Biochemistry and Biophysics Reports

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“…El contenido de sólidos solubles totales (SST) de los jugos se determinó de acuerdo con Corbin et al, (2015). El contenido de glucosa, fructosa y sacarosa en el jugo crudo se cuantificó mediante HPLC (Sandoval-Nuñez et al, 2018). La cuantificación de azúcares reductores se realizó por el método de Miller (Miller, 1959) utilizando un espectrofotómetro UV-vis (1280, Shimadzu, Japón) a una longitud de onda de 540 nm.…”

Section: Métodos Analíticosunclassified

Evaluation of acid-thermal pretreatments and enzymatic saccharification to obtain fermentable sugars from the juice and bagasse of Agave salmiana leaves

Martínez-Hernández

Hernández-Mendoza

Ruíz-Leza

et al. 2022

Renew. energ. biomass sustain

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This study presents the results of acid-thermal pretreatments and enzymatic saccharification for the production of fermentable sugars from the juice and bagasse of Agave salmiana leaves. In the acid-thermal pretreatment, H2SO4 was used while in the enzymatic saccharification the commercial enzyme Cellic Ctec2 (Novozyme) was used. In the results it was found that the best conditions of the acid thermal pretreatment (H2SO4 % (v/v) at 100 ° C for 30 min) for the juice generated a concentration of 43.65 ± 1.36 g/L of reducing sugars. In bagasse, the best conditions (H2SO4 % (p/v) at 120 ° C for 60 min) produced a concentration of 37.35 ± 1.65 g/L of reducing sugars in the hydrolysates. In the enzymatic saccharification at 15 FPU/g substrate and solids load of 10 % (p/v), a concentration of 87.37 ± 1.27 g/L of reducing sugars was obtained after 72 h. The results obtained demonstrate the potential of the integral use of bagasse and juices from the leaves of Agave salmiana as raw material to produce fermentable sugars.

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“…This strain has been viewed as an alternative to S. cerevisiae in 2nd generation ethanol processes, as it is able to naturally assimilate a variety of sugars in addition to glucose, such as pentose, hexose, arabinose, cellobiose, lactose and xylose, as well as some toxic compounds present in some sources of lignocellulosic biomass [ 74 , 75 ]. Pentjuss et al [ 76 ] applied and experimentally validated a stoichiometric model of central metabolism linked to biomass, to evaluate the efficiency of carbon conversion of K. marxianus .…”

Section: Conventional and Non-conventional Yeast Bioproduction Sysmentioning

confidence: 99%

The Model System Saccharomyces cerevisiae Versus Emerging Non-Model Yeasts for the Production of Biofuels

Lacerda

Eckert

2020

Life

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Microorganisms are effective platforms for the production of a variety of chemicals including biofuels, commodity chemicals, polymers and other natural products. However, deep cellular understanding is required for improvement of current biofuel cell factories to truly transform the Bioeconomy. Modifications in microbial metabolic pathways and increased resistance to various types of stress caused by the production of these chemicals are crucial in the generation of robust and efficient production hosts. Recent advances in systems and synthetic biology provide new tools for metabolic engineering to design strategies and construct optimal biocatalysts for the sustainable production of desired chemicals, especially in the case of ethanol and fatty acid production. Yeast is an efficient producer of bioethanol and most of the available synthetic biology tools have been developed for the industrial yeast Saccharomyces cerevisiae. Non-conventional yeast systems have several advantageous characteristics that are not easily engineered such as ethanol tolerance, low pH tolerance, thermotolerance, inhibitor tolerance, genetic diversity and so forth. Currently, synthetic biology is still in its initial steps for studies in non-conventional yeasts such as Yarrowia lipolytica, Kluyveromyces marxianus, Issatchenkia orientalis and Pichia pastoris. Therefore, the development and application of advanced synthetic engineering tools must also focus on these underexploited, non-conventional yeast species. Herein, we review the basic synthetic biology tools that can be applied to the standard S. cerevisiae model strain, as well as those that have been developed for non-conventional yeasts. In addition, we will discuss the recent advances employed to develop non-conventional yeast strains that are efficient for the production of a variety of chemicals through the use of metabolic engineering and synthetic biology.

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A comparative study of lignocellulosic ethanol productivities by Kluyveromyces marxianus and Saccharomyces cerevisiae

Cited by 24 publications

References 40 publications

Optimization of ethanol production using newly isolated ethanologenic yeasts

Optimization of ethanol production using newly isolated ethanologenic yeasts

Evaluation of acid-thermal pretreatments and enzymatic saccharification to obtain fermentable sugars from the juice and bagasse of Agave salmiana leaves

The Model System Saccharomyces cerevisiae Versus Emerging Non-Model Yeasts for the Production of Biofuels

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