Optimization of ethanol production using newly isolated ethanologenic yeasts

Tesfaw, Asmamaw; Öner, Ebru Toksoy; Assefa, Fassil

doi:10.1016/j.bbrep.2020.100886

Cited by 20 publications

(20 citation statements)

References 35 publications

(47 reference statements)

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“…Figure 4 shows that ethanol increased by the increase in the cell ratio and then decreased after reaching maximum ethanol content. The same phenomenon was also observed for fermentation time toward ethanol production [ 15 , 16 ].…”

Section: Resultssupporting

confidence: 74%

“…Temperature and fermentation time is also a key factor in yeast growth. Therefore, these parameters were also optimized for ethanol fermentation [ 15 , 16 ]. Response Surface Methodology (RSM) with a full factorial central composite design (CCD) was applied to optimize fermentation to maximize ethanol production and fermentation efficiency.…”

Section: Introductionmentioning

confidence: 99%

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Ethanol Production from Oil Palm Trunk: A Combined Strategy Using an Effective Pretreatment and Simultaneous Saccharification and Cofermentation

Wardani

Sutrisno

Faida

et al. 2021

International Journal of Microbiology

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Background. Oil palm trunk (OPT) with highly cellulose content is a valuable bioresource for bioethanol production. To produce ethanol from biomass, pretreatment is an essential step in the conversion of lignocellulosic biomass to fermentable sugars such as glucose and xylose. Several pretreatment methods have been developed to overcome biomass recalcitrance. In this study, the effects of different pretreatment methods such as alkali pretreatment, microwave-alkali, and alkaline peroxide combined with autoclave on the lignocellulosic biomass structure were investigated. Moreover, ethanol production from the treated biomass was performed by simultaneous saccharification and cofermentation (SSCF) under different temperatures, fermentation times, and cell ratios of Saccharomyces cerevisiae NCYC 479 and pentose-utilizing yeast, Pichia stipitis NCYC 1541. Results. Pretreatment resulted in a significant lignin removal up to 83.26% and cellulose released up to 80.74% in treated OPT by alkaline peroxide combined with autoclave method. Enzymatic hydrolysis of treated OPT resulted in an increase in fermentable sugar up to 93.22%. Optimization of SSCF by response surface method showed that the coculture could work together to produce maximum ethanol (1.89%) and fermentation efficiency (66.14%) under the optimized condition. Conclusion. Pretreatment by alkaline peroxide combined with autoclave method and SSCF process could be expected as a promising system for ethanol production from oil palm trunk and various lignocellulosic biomass.

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Section: Resultssupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Ethanol Production from Oil Palm Trunk: A Combined Strategy Using an Effective Pretreatment and Simultaneous Saccharification and Cofermentation

Wardani

Sutrisno

Faida

et al. 2021

International Journal of Microbiology

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“…The incubation period also tested (2,3and 4) days. The growth in different conditions was measured intermittently in OD (optical density at 600 nm) 17 .…”

Section: Optimization Of Ethanol Productionmentioning

confidence: 99%

Bioethanol Production by Candida tropicalis Isolated from Sheep Dung

Jafer

2023

Baghdad Sci.J

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Microorganisms have an active role in biotechnology for example yeasts, especially in some genus like Saccharomyces, Pichia, and Candida. C.tropicalis one of the most important species of Candida and despite it is one of the causative agents of candidiasis but it has a major role in the production of many chemical compounds. C.tropicalis in the previous study was isolated from sheep dung and morphologically and molecularly classified the result of sequencing was elucidate 100% similarity between the studied isolate and other isolates inserted in DNA Data Bank of Japan DDBJ, physiologically this isolate tolerated 6% ethanol concentration in broth media with the ability to the production of 4% ethanol under optimum conditions including (YEPDB) medium, incubation temperature 30◦C, PH 6 and dextrose as a carbon source which give more productivity as a carbon source and optimum incubation period was three days . This isolate gives a positive result for the cellulase enzyme-producing test and an exciting feature to remove effluent because it completely adsorbed the Congo red dye from dye solation in a liquid growth medium.

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“…Fermentas ETP22 and C. humilis ETP122 strains with survival rates of 65%, 60%, and 40%, respectively (Tesfaw et al, 2021).…”

Section: Effect Of Absolute Ethanol Stress Tolerancementioning

confidence: 99%

Characterization of Physicochemical and Rheological Properties on Qualities of “Tinsis” Culture and “Tella”: Ethiopian Traditional Fermented Beer.

Tesfaye

Tizazu

2022

Preprint

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PurposeTella is one of the most commonly consumed indigenous beverages in Ethiopia. The present study sought to isolate, identify wild yeast under species of Saccharomyces and characterize stress tolerance, physicochemical, rheological, and electrical properties on the quality of tella and "tinsis" culture during the pre- and post-fermentation storage period.MethodsSamples of Tella and “Tinsis” culture were collected from highly consumed areas and tinsis culture were also prepared in triplicate and physicochemical data are recorded, yeast media preparation, propagation, phenotypic characterization and stress tolerant test were followed simultaneously with data recording, and visualization using descriptive statistical analysis.ResultStarch degrading within short period of time, high flocculent, rapid growing, antifungal and antimicrobial resistant and with stand a 3-5 % acetic acid stressful environment and also resilient for killer toxins, yeasts were obtained from Tella and “Tinsis” culture. Yeast isolates from tella tolerated absolute ethanol up to 8-10%, pH from 3.5 to 4.5, hence could grow in osmotic concentration at 12% NaCL and survived in temperature 15 -35℃. Tella had more volatility, Redox potential (ORP) and low in salinity, sucrose content, total dissolved solid (TDS) and dynamic viscosity than that of tinsis culture. hence “tinsis” culture had highest through mean viscosity (7.92 ± 2.32cp), lactic acid (1.03 ± 0.38%) and volatile acetic acid 0.82 ± 0.30 and salinity 1.43 ± 0.43 psu) with low water activity (0.945 ± 0.019). moreover, as storage period increases the electrical resistivity influences with 90% that of redox potential and also resistivity correlates 28% with (ORP), and 46% with pH for tinsis culture. The rate of alcohol production increased with increase in redox potential, total dissolved solid and decreased by 53% with addition of 0.73% w/v of acetic acid to the 912ppm of (TDS) at pH 3.9. ConclusionTemperature, acidity and alkalinity regulation is critical tools to modify the speed of “Tinsis” culture and tella fermentation and for maintaining product shelf-life stability and Organolephtic taste. Species of S. Cerevisiae yeasts isolated from tella and tinsis revealed their potential abilities to substitute for commercial brewing industries.

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Optimization of ethanol production using newly isolated ethanologenic yeasts

Cited by 20 publications

References 35 publications

Ethanol Production from Oil Palm Trunk: A Combined Strategy Using an Effective Pretreatment and Simultaneous Saccharification and Cofermentation

Ethanol Production from Oil Palm Trunk: A Combined Strategy Using an Effective Pretreatment and Simultaneous Saccharification and Cofermentation

Bioethanol Production by Candida tropicalis Isolated from Sheep Dung

Characterization of Physicochemical and Rheological Properties on Qualities of “Tinsis” Culture and “Tella”: Ethiopian Traditional Fermented Beer.

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