The Model System Saccharomyces cerevisiae Versus Emerging Non-Model Yeasts for the Production of Biofuels

Lacerda, Maria Priscila Franco; Oh, Eun‐Suok; Eckert, Carrie A.

doi:10.3390/life10110299

Cited by 21 publications

(16 citation statements)

References 167 publications

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“…This has changed in the last few years with their increasing relevance for novel biotechnological processes and the enormous potential of the new discipline of synthetic biology [ 1 , 2 ]. Moreover, although the wine, beer, and baker’s yeast Saccharomyces cerevisiae holds a leading position not only in classical fermentations but also as a key model organism for eukaryotic cell biology [ 3 , 4 ], other “non-conventional” yeast species have been intensively studied as alternative microbial models and production organisms [ 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

The Pentose Phosphate Pathway in Yeasts–More Than a Poor Cousin of Glycolysis

2021

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The pentose phosphate pathway (PPP) is a route that can work in parallel to glycolysis in glucose degradation in most living cells. It has a unidirectional oxidative part with glucose-6-phosphate dehydrogenase as a key enzyme generating NADPH, and a non-oxidative part involving the reversible transketolase and transaldolase reactions, which interchange PPP metabolites with glycolysis. While the oxidative branch is vital to cope with oxidative stress, the non-oxidative branch provides precursors for the synthesis of nucleic, fatty and aromatic amino acids. For glucose catabolism in the baker’s yeast Saccharomyces cerevisiae, where its components were first discovered and extensively studied, the PPP plays only a minor role. In contrast, PPP and glycolysis contribute almost equally to glucose degradation in other yeasts. We here summarize the data available for the PPP enzymes focusing on S. cerevisiae and Kluyveromyces lactis, and describe the phenotypes of gene deletions and the benefits of their overproduction and modification. Reference to other yeasts and to the importance of the PPP in their biotechnological and medical applications is briefly being included. We propose future studies on the PPP in K. lactis to be of special interest for basic science and as a host for the expression of human disease genes.

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Section: Introductionmentioning

confidence: 99%

The Pentose Phosphate Pathway in Yeasts–More Than a Poor Cousin of Glycolysis

2021

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“…1A when one considers the branches with no apparent gene gains or losses is ‘what do we not know?’ Given the selective pressures in natural environments, and the range of solutions that evolution throws up, it seems improbable that we have uncovered the full extent of pathway rewiring, acquisition and loss of genes, and other changes. It is more likely that there are still many interesting and important features to be discovered and dissected in the less explored lineages and there is a strong case to continue to amass genomic data and undertake molecular studies in all Saccharomycetaceae lineages (Lacerda, Oh and Eckert 2020 ; Libkind et al . 2020 ).…”

Section: Perspectivesmentioning

confidence: 99%

Insights on life cycle and cell identity regulatory circuits for unlocking genetic improvement in Zygosaccharomyces and Kluyveromyces yeasts

Solieri

Cassanelli

Huff

et al. 2021

FEMS Yeast Research

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Evolution has provided a vast diversity of yeasts that play fundamental roles in nature and society. This diversity is not limited to genotypically homogenous species with natural interspecies hybrids and allodiploids that blur species boundaries frequently isolated. Thus, life-cycle and the nature of breeding systems have profound effects on genome variation, shaping heterozygosity, genotype diversity and ploidy level. The apparent enrichment of hybrids in industry-related environments suggests that hybridisation provides an adaptive route against stressors and creates interest in developing new hybrids for biotechnological uses. For example, in the Saccharomyces genus where regulatory circuits controlling cell-identity, mating competence and meiosis commitment have been extensively studied, this body of knowledge is being used to combine interesting traits into synthetic F1 hybrids, to by-pass F1 hybrid sterility, and to dissect complex phenotypes by bulk segregant analysis. Although there is less known about these aspects in other industrially-promising yeasts, advances in whole genome sequencing and analysis are changing this and new insights are being gained, especially in the food-associated genera Zygosaccharomyces and Kluyveromyces. We discuss this new knowledge and highlight how deciphering cell identity circuits in these lineages will contribute significantly to identify the genetic determinants underpinning complex phenotypes and open new avenues for breeding programmes.

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“…Analyzing and understanding oxygen requirements of facultatively fermentative ‘non-conventional’ yeasts can contribute to our comprehension of the roles of molecular oxygen in eukaryotic metabolism. In addition, such knowledge is essential for designing metabolic engineering strategies to enable application of non-conventional yeasts with industrially relevant traits, such as thermotolerance and inhibitor tolerance, in large-scale anaerobic processes (Lacerda, Oh and Eckert 2020 ; Sun and Alper 2020 ; Thorwall et al . 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Critical parameters and procedures for anaerobic cultivation of yeasts in bioreactors and anaerobic chambers

Mooiman

Bouwknegt

Dekker

et al. 2021

FEMS Yeast Research

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All known facultatively fermentative yeasts require molecular oxygen for growth. Only in a small number of yeast species, these requirements can be circumvented by supplementation of known anaerobic growth factors such as nicotinate, sterols and unsaturated fatty acids. Biosynthetic oxygen requirements of yeasts are typically small and, unless extensive precautions are taken to minimize inadvertent entry of trace amounts of oxygen, easily go unnoticed in small-scale laboratory cultivation systems. This paper discusses critical points in the design of anaerobic yeast cultivation experiments in anaerobic chambers and laboratory bioreactors. Serial transfer or continuous cultivation to dilute growth factors present in anaerobically pre-grown inocula, systematic inclusion of control strains and minimizing the impact of oxygen diffusion through tubing are identified as key elements in experimental design. Basic protocols are presented for anaerobic-chamber and bioreactor experiments.

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The Model System Saccharomyces cerevisiae Versus Emerging Non-Model Yeasts for the Production of Biofuels

Cited by 21 publications

References 167 publications

The Pentose Phosphate Pathway in Yeasts–More Than a Poor Cousin of Glycolysis

The Pentose Phosphate Pathway in Yeasts–More Than a Poor Cousin of Glycolysis

Insights on life cycle and cell identity regulatory circuits for unlocking genetic improvement in Zygosaccharomyces and Kluyveromyces yeasts

Critical parameters and procedures for anaerobic cultivation of yeasts in bioreactors and anaerobic chambers

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