A Comparative Study of Indirect Competitive ELISA and HPLC for Fumonisin Detection in Corn of the State of Paraná, Brazil

Ono, Elisabete Y. S.; Kawamura, Ossamu; Ono, Mário Augusto; Ueno, Yoshio; Hirooka, Elisa Yoko

doi:10.1080/09540100099580

Cited by 29 publications

(24 citation statements)

References 25 publications

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“…A GC method for quantification and confirmation of trichothecenes in wheat (40), TLC systems for the confirmation of trichothecenes (59) and a commercial ELISA kit for AFM 1 in milk (74) were assessed. Comparisons were carried out with ELISA and TLC methods for AFB 1 in samples of corn, feed and peanut (106) and for AFs, OTA and ZEA in corn and corn meal (63); two TLC methods for OTA in green coffee (66); and TLC and HPLC methods for AFM 1 in milk (116) and for FB S in corn (80). Screening methods for AFs in corn and peanut were also evaluated (44,83,119).…”

Section: Analytical and Mycological Methodsmentioning

confidence: 99%

Mycotoxin research in Brazil: the last decade in review

Rodriguez–Amaya

Sabino

2002

Braz. J. Microbiol.

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The number of research papers (128 papers) on mycotoxins published by Brazilian researchers in 1991-2000 surpassed the total number (85 papers) published in the preceding three decades . Thirty percent of the papers surveyed mycotoxins in foods and feeds. AFs in peanut and peanut products continued to be alarming, and high incidence and levels of FBs in corn and corn products also appeared as a serious problem. Contamination with other toxins, such as ZEA, OTA and trichothecenes, was low. Occurrence of AFM 1 in milk and dairy products and patulin in apple juice needs to be verified as the results are somewhat diverging. Work on analytical methods, mycological examination and toxic effects constituted 16, 13 and 13%, respectively, of the published papers in the decade assessed. Attempts to find means of preventing/controlling fungal growth and mycotoxin production notably increased, making up 27% of the papers, including investigations on influencing factors (e.g. genotype resistance, water content/a w , relative humidity, temperature, presence of metals, type of soil, mite infestation) and antagonistic potential of other microorganisms against mycotoxin-producing fungi. Effects of plant extract, flavonoids, fungicides and other chemicals, storage bag material, adsorbents, cooking and processing of food were also studied. Thus, notwithstanding constraints on resources, Brazilian research responds to the needs of the country, reflects international concerns and recent developments in the area.

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Section: Analytical and Mycological Methodsmentioning

confidence: 99%

Mycotoxin research in Brazil: the last decade in review

Rodriguez–Amaya

Sabino

2002

Braz. J. Microbiol.

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“…1). This value corresponded to mean absorbance plus three times the SD of the measurements of three replicate wells of 0 ng OTA/mL (Ono et al, 2000). The sensibility of ic-ELISA was adequate for detecting OTA in coffee samples.…”

Section: Resultsmentioning

confidence: 99%

“…4 correlated the data from OTA determination by HPLC and by ic-ELISA. Immunoassay recorded higher values of OTA than HPLC in every kind of coffees tested, indicating either recovery losses during sample preparation in HPLC (clean up step) and/or food matrix effect during immunoassay (Petska et al, 1994;Ono et al, 2000). Ochratoxin levels analyzed by ELISA were 0.66 to 1.46-fold higher than HPLC in green coffee (ELISA/HPLC ratio = 1.02), 0.96 to 1.11-fold in roasted coffee (ELISA/HPLC ratio = 1.02) and 0.93 to 1.82-fold in instant coffee (ELISA/HPLC ratio = 1.24) (Fig.…”

Section: Resultsmentioning

confidence: 99%

A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee

Fujii

Ono

Ribeiro

et al. 2007

Braz. arch. biol. technol.

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“…Assim, um grande número de amostras pode ser analisado em tempo reduzido e custo relativamente baixo. Estes métodos são promissores devido a sua simplicidade, que permite uma análise rápida para o controle de qualidade tanto no campo como na indústria (PESTKA; ABOUZEID; SUTIKNO, 1995;ABRAMSON, 2000;ONO et al, 2000).…”

Section: Métodos Para Detecção De Fungosunclassified

Imunoensaios: uma alternativa para a detecção de fungos toxigênicos em alimentos

et al. 2006

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ResumoO monitoramento constante da contaminação fúngica é imprescindível para assegurar a qualidade e segurança dos alimentos, reduzindo as perdas econômicas, assim como os riscos à saúde humana e animal. Os métodos tradicionais de identificação e detecção de fungos (cultivo em diversos meios, exame microscópico e análises bioquímicas) geralmente consomem muito tempo e exigem pessoal com experiência. Os imunoensaios, particularmente os ensaios imunoenzimáticos, constituem uma alternativa promissora aos métodos tradicionais devido à alta sensibilidade, especificidade, reprodutibilidade e potencial como método rápido de controle de qualidade. Dentre os ensaios imunoenzimáticos, aqueles baseados em exoantígenos são os mais empregados na resolução de problemas taxonômicos, detecção e identificação de fungos toxigênicos. Nesta revisão serão abordados conceitos básicos de imunoensaios, métodos de detecção de fungos, assim como diversos ensaios imunoenzimáticos para a detecção de fungos toxigênicos em alimentos. Palavras-chave: Imunoensaios, fungos toxigênicos, detecção de fungos, imunodetecção AbstractConstant monitoring of mould contamination is essential in order to assure the food quality and safety and reduce the economic losses, as well as to minimize the potential hazards to human and animal health. The traditional methods for mould identification and detection (culture in several media, microscopic examination and chemical analysis) are usually time-consuming and require trained staff. Immunoassays, particularly enzyme-linked immunosorbent assay (ELISA) could be a promising alternative to the traditional methods due to high sensitivity, specificity, reproducibility and potential for use in rapid quality control. Among ELISAs, those based on exoantigens are the most employed in the resolution of taxonomic problems, detection and identification of toxigenic fungi. This review discusses the basic principles of immunoassays, methods of mould detection and the several ELISAs developed for toxigenic fungi detection in food.

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A Comparative Study of Indirect Competitive ELISA and HPLC for Fumonisin Detection in Corn of the State of Paraná, Brazil

Cited by 29 publications

References 25 publications

Mycotoxin research in Brazil: the last decade in review

Mycotoxin research in Brazil: the last decade in review

A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee

Imunoensaios: uma alternativa para a detecção de fungos toxigênicos em alimentos

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