A comparative study of antioxidant enzyme activities in freshwater and seawater-adapted rainbow trout

Kolaylı, Sevgi; Keha, Edip

doi:10.1002/(sici)1099-0461(1999)13:6<334::aid-jbt7>3.0.co;2-m

Cited by 22 publications

(15 citation statements)

References 21 publications

(19 reference statements)

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“…The increased GP and CAT activities may be a compensatory mechanism to get rid of excess peroxides (Schlorff et al, 1999). A similar trend was reported (Kolayli and Keha (1999) between fresh water and seawater-adapted rainbow trout. It is also reported increase in CAT activity of fish erythrocytes in California Bight where contamination is higher, and an increase in GP activity in human and fish (Dicentrarchus labrax) erythrocytes in vitro a�er treatment with copper and mercury ions has been also reported (Brown et al, 1987).…”

Section: Resultssupporting

confidence: 78%

Effects of water reuse system on antioxidant enzymes of rainbow trout (Oncorhynchus mykiss w., 1792)

Özmen¹,

Bayır²,

Cengiz³

et al. 2004

Vet. Med. - Czech

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ABSTRACT:The objective of the present study was to investigate the importance of the antioxidant enzymes; glucose-6-phosphate dehydrogenase (G6PD), glutathione reductase (GR), superoxide dismutase (SOD), glutathione peroxidase (GP) and catalase (CAT) in defense against toxic reactive oxygen species (ROS). For this purpose, comparative studies were performed on the antioxidant enzyme activities in red cells of rainbow trout (Oncorhynchus mykiss) between flow-through and recirculated breeding systems G6PD, GR and SOD activities were found significantly lower in reuse system than flow-through system. But, GP and CAT activities were increased significantly in the erythrocyte of rainbow trout held in reuse water tanks.

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Section: Resultssupporting

confidence: 78%

Effects of water reuse system on antioxidant enzymes of rainbow trout (Oncorhynchus mykiss w., 1792)

Özmen¹,

Bayır²,

Cengiz³

et al. 2004

Vet. Med. - Czech

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“…The more absorbance decreases, the higher the activity is. To study the effect of PN and DPN on CAT enzyme, five different concentrations (10,20,40, 50 and 100 mM) of PN and DPN were used. Absorbance change as a result of PN addition to the medium was shown in the graphics in Figure 1.…”

Section: Resultsmentioning

confidence: 99%

“…First, the homogenate containing CATenzyme at constant concentration was treated with PN at various concentrations (10,20,40, 50 and 100 mM), and the CAT activity was then measured. For blank, untreated enzyme was used.…”

Section: Effect Of Pn and Dpn On Cat Activitymentioning

confidence: 99%

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In vitro effects of peroxynitrite treatment on fish liver catalase activity

Sivrikaya

Kolaylı

Küçük

et al. 2009

Journal of Enzyme Inhibition and Medicinal Chemistry

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The effect of peroxynitrite (PN), a highly toxic agent, on catalase (CAT) activity in fish liver microsomal homogenates was determined. PN was synthesized by mixing acidic hydrogen peroxide solution with sodium nitrite solution and then adding sodium hydroxide solution into the mixture in order to stabilize the highly labile compound peroxynitrous acid (ONOOH) in peroxynitrite anion form (ONOO 2 ). The effect of PN and decomposed peroxynitrite (DPN), prepared by preincubation with HCl, was monitored by using a constant amount of homogenate containing the CATenzyme. Significant losses were observed in the CATactivity of fish liver enzyme after treatment with PN and also with DPN products, the inhibitory effect of PN being slightly more pronounced than that of DPN. IC 50 values were 5.5 and 8.5 mM for PN and DPN, respectively. The PN inhibition of CAT activity is due to both the effects of the secondary and decomposition products of PN and its nitration and oxidation effects on the amino acid residues of the enzyme.

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“…Small amounts of pesticides can cause sublethal damage to many organisms, but little is known about how their affects on specific enzymes in fish such as the sturgeon investigated here. There are only very few literature reports regarding the interaction of pollutants with fish enzymes critical for physiological processes, such as the CAs 2,10,11 . In this study, we report the purification, characterization and inhibition of a CA from the endangered sturgeon species, Acipenser gueldenstaedti.…”

Section: Short Communicationmentioning

confidence: 99%

Characterization and inhibition studies of an α-carbonic anhydrase from the endangered sturgeon speciesAcipenser gueldenstaedti

Kolaylı

Karahalil

Şahin

et al. 2011

Journal of Enzyme Inhibition and Medicinal Chemistry

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A comparative study of antioxidant enzyme activities in freshwater and seawater-adapted rainbow trout

Cited by 22 publications

References 21 publications

Effects of water reuse system on antioxidant enzymes of rainbow trout (Oncorhynchus mykiss w., 1792)

Effects of water reuse system on antioxidant enzymes of rainbow trout (Oncorhynchus mykiss w., 1792)

In vitro effects of peroxynitrite treatment on fish liver catalase activity

Characterization and inhibition studies of an α-carbonic anhydrase from the endangered sturgeon speciesAcipenser gueldenstaedti

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