Effects of water reuse system on antioxidant enzymes of rainbow trout (Oncorhynchus mykiss w., 1792)

Özmen, İsmail; Bayır, Abdulkadir; Cengiz, Müjgan; Sirkecioğlu, Ahmet; Atamanalp, Muhammed

doi:10.17221/5726-vetmed

Cited by 36 publications

(17 citation statements)

References 29 publications

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“…These enzyme systems have been proposed as biomarkers of reactive oxygen species (ROS) mediating contaminant exposure and as a potential tool in environmental risk assessment (Kohen and Nyska 2002;Livingstone 2001). The ROS are detoxified by a set of antioxidant enzymes that protect macromolecules such as proteins, lipids and nucleic acids against damage (Lushchak et al 2001;Ozmen et al 2004). Several studies revealed that exposure to contaminants, including Cd, in aquatic ecosystems can enhance the intracellular formation of ROS which could cause oxidative damage to biological systems (Ercal et al 2001;Ferreira et al 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Oxidized glutathione (GSSG) should be reduced to GSH by the ancillary flavor-enzyme glutathione reductase, which uses NADPH as electron donor (Hasspieler et al 1994). SOD is the enzyme that catalyzes the dismutation of the superoxide anion to O 2 and H 2 O 2 , and CAT reacts with H 2 O 2 to form water and oxygen molecule (Livingstone 2001;Lushchak et al 2001;Ozmen et al 2004). The GPx detoxifies H 2 O 2 or organic hydroperoxides that produced in LPO (Halliwell and Gutteridge 1999).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of tissue-specific effect of cadmium on antioxidant defense system and lipid peroxidation in freshwater murrel, Channa punctatus

Dabas

Nagpure

Kumar

et al. 2011

Fish Physiol Biochem

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The effects of different concentrations of cadmium chloride on the extent of lipid peroxidation (LPO) and alterations in the antioxidant enzyme activities were studied in liver, kidney and gill tissues of freshwater murrel, Channa punctatus. The fish specimens were exposed to 6.7, 13.4 and 20.1 mg l(-1) sublethal concentrations of cadmium chloride and the oxidative stress was assessed after 24, 48, 72 and 96 h post-exposure. The biomarkers selected for the study were thiobarbituric acid reactive substances for assessing the extent of lipid peroxidation and antioxidant defense system such as reduced glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GP(X)), glutathione-S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD) activities. In general, the cadmium exposure elevated the LPO in subject tissues of treated group and modulated the activities of GPx, GST, SOD, CAT, GR and level of GSH after given exposure as compared to the control. All enzymes activities, except CAT (in kidney and gills), and amount of LPO elevated significantly (P < 0.05) in treated group with respect to control in all tissues, while significant difference was not observed between the exposed concentrations and within exposure duration. The results indicated that increase in LPO level and the fluctuation in antioxidant defense system in fish could be due to cadmium-induced increase in the production of reactive oxygen species (ROS). The potential role of these parameters as biomarkers of heavy metal pollution in aquatic system is discussed.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of tissue-specific effect of cadmium on antioxidant defense system and lipid peroxidation in freshwater murrel, Channa punctatus

Dabas

Nagpure

Kumar

et al. 2011

Fish Physiol Biochem

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“…When statistically examined the effect of application, species and application x species interaction on GR is found to be insignificant. The effects of recirculation system on antioxidant enzymes of rainbow trout and when GR enzyme is compared with control group the result were found very important (Ozmen et al, 2004). Compared with control group for catalase enzyme, the values obtained by application of phenoxyethanol with brown trout are close to normal, but lower in rainbow trout.…”

Section: Resultsmentioning

confidence: 85%

“…G6PD activity changes depending on nutrition, hormones and especially NADPH concentration. Ozmen et al, 2004 inspected the effects of recirculation system on antioxidant enzymes of rainbow trout and when G6PD enzyme is compared with control group the result were found very important. In the application groups, a significant increase was observed in G6PD enzyme activity as it stops the enzyme synthesis.…”

Section: Resultsmentioning

confidence: 99%

Effects of Anesthetic Substances on Some Antioxidan Enzyme Activities of Trouts

Uçar¹,

Atamanalp²,

Çankaya

et al. 2013

J FisheriesSciences.com

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In this study where the effects of naturel (clove oil) and synthetic (2-phenoxyethanol) anesthetic substances were investigated, rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta fario) were used as fish material. In the blood samples taken from the fishes exposed to the doses of both compounds for 28 days, enzyme activity values were inspected. When enzyme activity values are considered, although the effect of anesthetic substance application on glucose 6-phosphate dehydrogenase and 6-phospho gluconate dehydrogenase enzymes have been found to be important (p<0.05), the type and interaction have been found unimportant (p>0.05). The effect of type on the glutathione reductase enzyme activity values is found to be important (p<0.05), whereas catalase enzyme is not effected by application, type and interaction. Clove oil showed inhibition effects on glutathione reductase but 2-phenoxyethanol did not. 2-phenoxyethanol did not exhibit inhibition on catalase enzyme from brown trout.Bu çalışmada naturel (karanfil yağı) ve sentetik (2-fenoksietanol) anestezik maddelerin etkileri araştırıldı. Gökkuşağı alabalığı (Oncorhynchus mykiss) ve kahverengi alabalık (Salmo trutta Dere) kullanılmıştır. 28 gün boyunca her iki bileşik belli dozlarda balıklar üzerinde uygulandı ve balıklardan alınan kan örneklerinde, enzim aktivitesi değerleri incelendi. Enzim aktivitesi değerleri dikkate alındığında, glukoz 6-fosfat dehidrogenaz ve 6-fosfor glukonat dehidrogenaz enzimleri üzerine anestezik madde uygulamasının etkisinin önemli olduğu tespit edilmiş (p<0.05) olmasına rağmen tipi ve etkileşim önemsiz bulunmuştur (p>0.05). Glutatyon redüktaz enzim aktivitesi değerleri üzerine etkisi önemli bulunmuştur (p<0.05), ancak katalaz enzimi uygulamasında her hangi bir etki yapmamıştır. Karanfil yağı glutatyon redüktaz üzerindeki inhibisyon etkileri gösterdi ancak 2-fenoksietanol her hangi bir etki göstermedi. 2-fenoksietanol, kahverengi alabalıkta uygulandığında katalaz enzimi üzerinde inhibisyon gözlenmemiştir.

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“…Lipid peroxidation (LPO) as induced by heavy metal pollution in aquatic organisms is expressed by malondialdehyde (MDA) formation, which represents the secondary LPO product in the thiobarbituric acid reactive substances (TBARS) test (Draper et al 1993). ROS are detoxified as antioxidant defense systems including antioxidants like reduced glutathione and a set of antioxidant enzymes such as glutathione peroxidase, glutathione-S-transferase, superoxide dismutase, and catalase that protect macromolecules against oxidative damage (Ozmen et al 2004). Exposure to contaminants, including Cd, in aquatic ecosystems can enhance intracellular formation of ROS, which could cause oxidative damage to biological systems (Ferreira et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Investigation of Cadmium-Induced Genotoxicity and Oxidative Stress Response in Indian Major Carp,Labeo rohita

Dabas

Nagpure

Mishra

et al. 2013

Human and Ecological Risk Assessment: An International Journal

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We investigated genotoxicity and oxidative stress in the gills of Labeo rohita exposed to 33.6, 67.1, and 100.6 mg L -1 of cadmium chloride at 96 h. Genotoxicity was assessed using single cell gel electrophoresis whereas oxidative stress was monitored through lipid peroxidation induction and antioxidant response parameters, namely reduced glutathione (GSH), glutathione peroxidase, glutathione-S-transferase, superoxide dismutase, and catalase (CAT) activities. Significant (p < .05) effect of both concentration and time of exposure was observed on the extent of DNA damage in treated fish. Similarly, malondialdehyde content, level of GSH, and activities of antioxidant enzymes were significantly elevated in treated groups, except CAT. The increased DNA damage and lipid peroxidation (LPO) content along with fluctuation in antioxidant defense system in fish indicated the interaction of cadmium (Cd) with DNA repair processes and production of reactive oxygen species. Thus, Cd is liable for induction of LPO, alteration of antioxidant defenses, and DNA damage in gills of L. rohita.

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Effects of water reuse system on antioxidant enzymes of rainbow trout (Oncorhynchus mykiss w., 1792)

Cited by 36 publications

References 29 publications

Assessment of tissue-specific effect of cadmium on antioxidant defense system and lipid peroxidation in freshwater murrel, Channa punctatus

Assessment of tissue-specific effect of cadmium on antioxidant defense system and lipid peroxidation in freshwater murrel, Channa punctatus

Effects of Anesthetic Substances on Some Antioxidan Enzyme Activities of Trouts

Investigation of Cadmium-Induced Genotoxicity and Oxidative Stress Response in Indian Major Carp,Labeo rohita

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