A Comparative Proteomic Analysis of Human and Rat Embryonic Cerebrospinal Fluid

Zappaterra, Mauro D.; Lisgo, Steven; Lindsay, Susan; Gygi, Steven P.; Walsh, Christopher A.; Ballif, Bryan A.

doi:10.1021/pr070247w

Cited by 122 publications

(145 citation statements)

References 44 publications

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“…Cerebrospinal fluid (CSF) has been reported to contain nerve growth factor (NGF) and transforming growth factor alpha (TGF-alpha) (35). Although the role of the CSF during embryogenesis is just starting to be studied, several recent papers suggest an important role for CSF in brain development.…”

Section: Immediately After Electrophysiological Evaluation Middlementioning

confidence: 99%

The effect of cerebro-spinal fluid in collagen guide channel on sciatic nerve regeneration in rat

Farjah

Dolatkhah²,

Pourheidar³

et al. 2015

Turkish Neurosurgery

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ABSTRACTgaps by inserting the severed nerve stumps into the two ends of the canal (16). Some natural and synthetic substances can be shaped in structures that are able to promote nerve regeneration for example: eggshell membrane (12), silicone (31), human amnion layer (22), collagen (18), collagen-Chitosan (36).Collagen-based biomaterials have been extensively studied as a promising nerve guide (7). Since collagen is a natural material, it shows excellent biocompatibility, insignificant immunogenicity, and high bio-absorbability (27).█ INTRODUCTION F or peripheral nerve repair, nerve autografts have always been considered as the "gold standard" for the restoration of structural and functional nerve regeneration (34).Autograft has several disadvantages, including the need for an extra-incision, loss of donor nerve function, mismatch in size between the donor nerve and the injured nerve, and a limited availability of donor nerve (33). To solve these problems, nerve guide channels (NGCs) have been made to bridge the nerve AIm: The aim of this study was to evaluate the effect of cerebrospinal fluid (CSF) in nerve regeneration across the collagen guide channel in comparison with autograft. mATERIAl and mEThODS: Forty adult male rats (250-300 g) were randomized into (1) collagen channel+CSF, (2) collagen channel+normal saline (NS), (3) autograft, and (4) sham surgery groups. The left sciatic nerve was exposed and a 10 mm nerve segment was cut and removed. In the collagen groups, the proximal and distal cuts ends of sciatic nerve were telescoped into the nerve guides and CSF or NS injected into collagen conduit. In the autograft group, the 10 mm nerve segment was turned backwards and used an autologous nerve graft. All animals were evaluated by sciatic functional index (SFI) and electrophysiology, histology, and immunohistochemistry testing. RESUlTS:The improvements in SFI since the beginning of the last evaluation in experimental groups were measured. On days 49 and 60 post-operation, the mean SFI of the collagen+CSF group was significantly greater than the autograft group (P< 0.05). On day 90, the mean nerve conduction velocity (NCV) of the collagen+CSF group was greater than autograft group (P< 0.05). The number of myelinated fibers in the collagen+CSF group was significantly greater than that of the collagen + NS group at day 90 (P<0.05).CONClUSION: CSF in collagen nerve guide channel effectively enhances nerve regeneration and promotes functional recovery in injured sciatic nerve of rats.

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Section: Immediately After Electrophysiological Evaluation Middlementioning

confidence: 99%

The effect of cerebro-spinal fluid in collagen guide channel on sciatic nerve regeneration in rat

Farjah

Dolatkhah²,

Pourheidar³

et al. 2015

Turkish Neurosurgery

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“…Il est intéressant de noter que des MP d'origine gliale ont été identifiées dans le LCR au cours de la sclérose en plaque [25]. Cette démarche paraît d'autant plus prometteuse que la détection et l'analyse des microparticules spécifiquement liées aux AVC pourraient bénéficier de techniques protéomiques [26][27][28]. La valeur clinique de ce biomarqueur dépendra de son pouvoir à identifier des individus à haut risque avant toute manifestation clinique de la maladie ; si tel est le cas, les MP cellulaires pourraient constituer un autre outil essentiel dans la pré-vention, le diagnostic et le suivi thérapeutique des accidents vasculaires cérébraux ischémiques [17].…”

Section: Sang Circulant Parenchyme Cérébralunclassified

Ischémie cérébrale : les microparticules neurovasculaires, une alternative aux marqueurs biologiques sanguins

Anglès-Cano

Vivien

2009

Med Sci (Paris)

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843 SYNTHÈSE REVUES Marqueurs biologiques des accidents vasculaires cérébraux ischémiquesLe cerveau se trouve sous la dépendance d'un apport continu de glucose et d'oxygène par le sang circulant. Lorsque le débit sanguin chute au-dessous d'une certaine limite, la combinaison d'une hypoperfusion et d'un déficit énergétique conduit à un arrêt réversible du fonctionnement neuronal dans la zone affectée, appelée pénombre ischémique. Le devenir de cette zone dépend de la restauration précoce de la perfusion cérébrale. Lorsqu'elle s'accentue ou se prolonge, l'ischémie entraîne la mort, ou nécrose, du tissu cérébral. On appelle infarctus cérébral un foyer d'ischémie cérébrale associant des zones de nécrose et de pénombre. Ces accidents vasculaires cérébraux (AVC) ischémiques surviennent, pour la plupart, dans un contexte d'athérothrombose le plus > L'intérêt clinique des biomarqueurs est de pouvoir identifier des individus à risque de dévelop-per une maladie afin d'établir des mesures pré-ventives, diagnostiques ou thérapeutiques. Or, nous manquons actuellement d'un test rapide, sensible et pratique pour le diagnostic de l'ictus ischémique aigu. Un nombre important de molé-cules circulantes ont été associées aux accidents vasculaires cérébraux (AVC) ischémiques, sans qu'aucun consensus n'établisse leur utilité. En effet, ces molécules ne sont pas spécifiques du complexe neurovasculaire altéré dans l'AVC et pourraient également témoigner d'autres pathologies vasculaires. Même l'association de certains de ces marqueurs biologiques (métallo-protéinase matricielle 9, peptide natriurétique de type B, D-dimères, protéine S100B…) n'a pas permis d'obtenir des informations diagnostiques et pronostiques supplémentaires. Récemment, un nouveau type de biomarqueur, les microparticules, fragments cellulaires émis par des cellules en souffrance, s'avère être potentiellement intéressant et suffisamment robuste. Les diffé-rents déterminants antigéniques et les effecteurs moléculaires portés par ces microparticules qui, par leur origine, sont spécifiques de la cellule activée ou lésée, pourraient permettre l'identification précoce du tissu affecté. Ces microparticules pourraient être détectées non seulement dans le liquide céphalorachidien, mais aussi dans les larmes ou dans la circulation sanguine en cas de dysfonctionnement de la barrière hémato-encéphalique. Il est donc essentiel d'évaluer leur rôle de marqueur biologique dans la préven-tion, le diagnostic et le suivi thérapeutique des accidents vasculaires cérébraux ischémiques. < Inserm U919, Sérine protéases et physiopathologie de l'unité neurovasculaire,

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“…The CSF is a complex fluid that bathes the developing neuroepithelium [1][2][3][4][5][6] and provides essential pressure 7 and growth promoting cues for the developing brain [8][9][10][11][12] . To study the CSF over the course of brain development, we developed techniques to isolate ventricular CSF from developing rat or mouse embryos during various stages of development 6,9 .…”

Section: Introductionmentioning

confidence: 99%

“…To study the CSF over the course of brain development, we developed techniques to isolate ventricular CSF from developing rat or mouse embryos during various stages of development 6,9 . Previous methods of isolation included using a glass micro-needle and isolating the CSF using a micro-injector 1,2 .…”

Section: Introductionmentioning

confidence: 99%

Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants

Zappaterra

LaMantia

Walsh

et al. 2013

JoVE

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The CSF is a complex fluid with a dynamically varying proteome throughout development and in adulthood. During embryonic development, the nascent CSF differentiates from the amniotic fluid upon closure of the anterior neural tube. CSF volume then increases over subsequent days as the neuroepithelial progenitor cells lining the ventricles and the choroid plexus generate CSF. The embryonic CSF contacts the apical, ventricular surface of the neural stem cells of the developing brain and spinal cord. CSF provides crucial fluid pressure for the expansion of the developing brain and distributes important growth promoting factors to neural progenitor cells in a temporally-specific manner. To investigate the function of the CSF, it is important to isolate pure samples of embryonic CSF without contamination from blood or the developing telencephalic tissue. Here, we describe a technique to isolate relatively pure samples of ventricular embryonic CSF that can be used for a wide range of experimental assays including mass spectrometry, protein electrophoresis, and cell and primary explant culture. We demonstrate how to dissect and culture cortical explants on porous polycarbonate membranes in order to grow developing cortical tissue with reduced volumes of media or CSF. With this method, experiments can be performed using CSF from varying ages or conditions to investigate the biological activity of the CSF proteome on target cells.

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A Comparative Proteomic Analysis of Human and Rat Embryonic Cerebrospinal Fluid

Cited by 122 publications

References 44 publications

The effect of cerebro-spinal fluid in collagen guide channel on sciatic nerve regeneration in rat

The effect of cerebro-spinal fluid in collagen guide channel on sciatic nerve regeneration in rat

Ischémie cérébrale : les microparticules neurovasculaires, une alternative aux marqueurs biologiques sanguins

Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants

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