Little is known about the mechanism of mitochondrial division. We show here that mitochondria are disrupted by mutations in a C. elegans dynamin-related protein (DRP-1). Mutant DRP-1 causes the mitochondrial matrix to retract into large blebs that are both surrounded and connected by tubules of outer membrane. This indicates that scission of the mitochondrial outer membrane is inhibited, while scission of the inner membrane still occurs. Overexpressed wild-type DRP-1 causes mitochondria to become excessively fragmented, consistent with an active role in mitochondrial scission. DRP-1 fused to GFP is observed in spots on mitochondria where scission eventually occurs. These data indicate that wild-type DRP-1 contributes to the final stages of mitochondrial division by controlling scission of the mitochondrial outer membrane.
BackgroundSomatic mutations in the kinase domain of the epidermal growth factor receptor tyrosine kinase gene EGFR are common in lung adenocarcinoma. The presence of mutations correlates with tumor sensitivity to the EGFR inhibitors erlotinib and gefitinib, but the transforming potential of specific mutations and their relationship to drug sensitivity have not been described.Methods and FindingsHere, we demonstrate that EGFR active site mutants are oncogenic. Mutant EGFR can transform both fibroblasts and lung epithelial cells in the absence of exogenous epidermal growth factor, as evidenced by anchorage-independent growth, focus formation, and tumor formation in immunocompromised mice. Transformation is associated with constitutive autophosphorylation of EGFR, Shc phosphorylation, and STAT pathway activation. Whereas transformation by most EGFR mutants confers on cells sensitivity to erlotinib and gefitinib, transformation by an exon 20 insertion makes cells resistant to these inhibitors but more sensitive to the irreversible inhibitor CL-387,785.ConclusionOncogenic transformation of cells by different EGFR mutants causes differential sensitivity to gefitinib and erlotinib. Treatment of lung cancers harboring EGFR exon 20 insertions may therefore require the development of alternative kinase inhibition strategies.
During vertebrate central nervous system development, the apical neuroepithelium is bathed with
embryonic Cerebrospinal Fluid (e-CSF) which plays regulatory roles in cortical cell proliferation and
maintenance. Here, we report the first proteomic analysis of human e-CSF and compare it to an extensive
proteomic analysis of rat e-CSF. As expected, we identified a large collection of protease inhibitors,
extracellular matrix proteins, and transport proteins in CSF. However, we also found a surprising suite
of signaling and intracellular proteins not predicted by previous proteomic analysis. Some of the
intracellular proteins are likely to represent the contents of microvesicles recently described within the
CSF (Marzesco, A. M., et al. J. Cell Sci. 2005, 118 (Pt. 13), 2849−2858). Defining the rich composition of
e-CSF will enable a greater understanding of its concerted actions during critical stages of brain
development.
Keywords: embryonic CSF (e-CSF) • human CSF • rat CSF • brain development • cerebrospinal fluid • mass
spectrometry • proteomics
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