A comparative morphometric study of the myocardium during the postnatal development in normotensive and spontaneously hypertensive rats

Iliev, Alexandar; Kotov, Georgi; Landzhov, Boycho; Jelev, Lazar; Kirkov, Vidin; Hinova-Palova, Dimka

doi:10.5603/fm.a2017.0094

Cited by 12 publications

(12 citation statements)

References 27 publications

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“…It is often associated with increased morbidity from atherosclerotic disease, stroke and multiple other cardiovascular and cerebrovascular events 1 . Spontaneously hypertensive rats (SHR) are often used as a model of arterial hypertension in humans and allow researchers to study and modify the connection between hypertension as the initiating factor and alterations in myocardial morphology and function as a consequence to it [2][3][4] . Cardiomyocytic hypertrophy, focal myocytolysis and ventricular fibrosis are only a part of the alterations in the morphology of the myocardium observed in spontaneously hypertensive rats with the progression of hypertension.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of structural myocardial changes during chronic hypertensive states in rats

Iliev¹,

Kotov²,

Dimitrova³

et al. 2018

J Cardiol and Cardiovasc Sciences

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Introduction: Spontaneously hypertensive rats are often used as a model of arterial hypertension in humans. Cardiomyocytic hypertrophy, focal myocytolysis and ventricular fibrosis are only a part of the alterations in the morphology of the myocardium observed in spontaneously hypertensive rats with the progression of hypertension. The present manuscript reviews our studies on spontaneously hypertensive rats, with focus on the microscopic changes in the myocardial architectonics and analysis of several morphometric parameters. Materials and methods: A total of 12 male spontaneously hypertensive rats, distributed in two age groups, each containing six animals: 1-monthold (young) and 6-months-old (adult) were used. We also used 12 male normotensive Wistar rats, distributed in two age groups, each containing six animals: 1-month-old (young) and 6-months-old (adult). Routine haematoxylin and eosin staining and Mallory's trichrome stain were conducted. Quantitative data were obtained with a computerized system for image analysis NIS-Elements Advanced Research (Ver. 2.30). Results: Changes in the normal morphology of the myocardium included cardiomyocytic hypertrophy, focal myocytolysis and ventricular fibrosis. As aging progressed, we noted a significant increase in the thickness of the free wall and cross-sectional area of the cardiomyocytes and the cardiomyocytic nuclei and a decrease in cardiomyocytic density. Conclusion: The manuscript presents a detailed qualitative and quantitative study of changes in the normal structure of the myocardium initiated by arterial hypertension.

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Section: Introductionmentioning

confidence: 99%

Evaluation of structural myocardial changes during chronic hypertensive states in rats

Iliev¹,

Kotov²,

Dimitrova³

et al. 2018

J Cardiol and Cardiovasc Sciences

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“…As it was determined earlier, the characteristic signs of age-related remodeling of the myocardium in SHR are an increase in the area of cardiomyocytes and their nuclei, as well as a decrease in the density of cardiomyocytes in comparison with normotensive animals [8,9]. Myocardial hypertrophy in rats of this strain, reaching a 30% increase in heart weight, occurs as a compensatory response to a constant increase in systemic arterial pressure [10,11].…”

Section: Discussionmentioning

confidence: 70%

“…At the initial stages of PTU intake, dystrophic processes developed in the myocardium of rats, which were manifested by a decrease in the area of the cytoplasm of cardiomyocytes. However, at the PTU intake for no more than a month, these changes did not go beyond the cardiomyocytes in normotensive rats [9]. A longer intake of PTU was manifested in a further decrease in the area of the cardiomyocyte cytoplasm and pycnosis of the nuclei.…”

Section: Discussionmentioning

confidence: 80%

Morphological Parameters of the Myocardium in Spontaneously Hypertensive Rats upon Administration of Propylthiouracil

Pobelensky

Kolot

Protsenko

et al. 2021

Recent Adv. Biol. Med.

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The morphological changes in myocardial tissue in spontaneously hypertensive rat (SHR) occurring after administration of propylthiouracil (PTU) were assessed in the project. PTU at a concentration of 0.1% was given to the animals with drinking water for 47 days. After the end of the experiment, the morphological parameters of cardiomyocytes, the density of cardiomyocytes and fibroblasts in the myocardium, and the average area of the lumen of veins were examined. The progressive development of dystrophic processes in the myocardium was established against the background of PTU intake, which was characterized by a decrease in the cardiomyocyte density and an increase in the volume of connective tissue, venous congestion, and expansion of the lumen of veins.

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“…Hypertension is a common condition with important social and clinical signifi cance, which is characterized by various morphological alterations in the target organs of the cardiovascular and urinary system, associated with a decrease in the functional capacity of the heart and kidney. These changes include left ventricular hypertrophy, cardiac fi brosis, pronounced glomerulosclerosis, thickening of the glomerular and tubular basement membranes, as well as renal interstitial fi brosis (1)(2)(3). Fibrosis is mainly of the reactive type, but a co-existence between reactive and reparative cannot be excluded (4).…”

Section: Introductionmentioning

confidence: 99%

Differential collagen expression in kidney and heart during hypertension

Stanchev¹,

Stamenov²,

Kirkov³

et al. 2020

BLL

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The aim of the present study was to investigate the immunohistochemical expression of selected collagen types, namely collagen types I and V and procollagen type III in the renal parenchyma and interstitium and in the myocardium of spontaneously hypertensive rats. MATERIAL AND METHODS: For the present study, we used two age groups of 6-and 12-month-old spontaneously hypertensive rats. An immunohistochemical analysis was conducted with monoclonal antibodies against collagen types I and V and procollagen type III. A semi-quantitative analysis of immunostaining intensity was conducted with the Image J software. RESULTS: In the kidney, all three molecules showed higher expression at the age of 12 months, which was particularly notable for procollagen type III and collagen type V, which stained as highly-positive. In the myocardium, the immunoreactivity of collagen types I and V was stronger in 12-month-old animals, while that of procollagen type III did not change substantially. CONCLUSION: The present study suggests a role of collagen types III and V in hypertensive kidney disease, while also establishing the role of increased expression of collagen types I and V in adverse myocardial remodeling (Tab. 1, Fig. 2, Ref. 48).

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A comparative morphometric study of the myocardium during the postnatal development in normotensive and spontaneously hypertensive rats

Abstract: Background: Differences in the size of cardiac muscle cells observed in normal and

Cited by 12 publications

References 27 publications

Evaluation of structural myocardial changes during chronic hypertensive states in rats

Evaluation of structural myocardial changes during chronic hypertensive states in rats

Morphological Parameters of the Myocardium in Spontaneously Hypertensive Rats upon Administration of Propylthiouracil

Differential collagen expression in kidney and heart during hypertension

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