A Comparative Metabolomics Analysis Reveals the Tissue-Specific Phenolic Profiling in Two Acanthopanax Species

Abstract

Acanthopanax senticosus (Rupr. Maxim.) Harms (ASH) and Acanthopanax sessiliflorus (Rupr. Maxim.) Seem (ASS), are members of the Araliaceae family, and both are used in Asian countries. These herbals have drawn much attention in recent years due to their strong biological activity, with innocuity and little side effects. However, the common and distinct mode of compound profiles between ASH and ASS is still unclear. In this study, a high performance liquid chromatograph-mass spectrometry (HPLC-MS) method was developed to simultaneously quantify the seven major active compounds, including protocatechuate, eleutheroside B, eleutheroside E, isofraxidin, hyperoside, kaempferol and oleanolic acid. Then the targeted metabolomics were conducted to identify 19 phenolic compounds, with tight relation to the above mentioned active compounds, including nine C6C3C6-type, six C6C3-type and four C6C1-type in the two Acanthopanax species studied here. The results showed that the seven active compounds presented a similar trend of changes in different tissues, with more abundant accumulation in roots and stems for both plants. From the view of plant species, the ASH plants possess higher abundance of compounds, especially in the tissues of roots and stems. For phenolics, the 19 phenols detected here could be clearly grouped into five main clusters based on their tissue-specific accumulation patterns. Roots are the tissue for the most abundance of their accumulations. C6C3C6-type compounds are the most widely existing type in both plants. In conclusion, the tissue- and species-specificity in accumulation of seven active compounds and phenolics were revealed in two Acanthopanax species.

A comparative metabolomics analysis of the components of heartwood and sapwood in Taxus chinensis (Pilger) Rehd.

“…The MS parameter was set as described previously 16,18 . In brief, the temperature of ESI source was 500 °C; the voltage of ion spray was 5500 V; ion source gas I, gas II and curtain gas were set at 55, 60, and 25.0 psi, respectively; the collision gas was set to high.…”

“…Metabolomics is a valuable approach for the high-throughput and comprehensive study of complex metabolic compositions, and has been widely applied in plants 16–20 . Mass spectrometry methods have been used for detection and quantification of metabolites 16–18 . With the aim to investigate the metabolites variation between the SW and HW in T. chinensis , widely targeted metabolomics approach using liquid chromatography tandem mass spectrometry (LC-MS/MS) was performed.…”

Geographical Distribution and Environmental Correlates of Eleutherosides and Isofraxidin in Eleutherococcus senticosus from Natural Populations in Forests at Northeast China

“…The methodology of bioactive compound extraction was modified from that of Wu et al [20]. Samples were oven-dried at 45 • C for h and ground to pass a 60-mesh sieve (0.25 mm).…”

Changes in Growth and Photosynthetic Parameters and Medicinal Compounds in Eleutherococcus senticosus Harms under Drought Stress

“…The analysis of compounds is described in our previous studies (Wu et al, 2018); roots and stems were pulverized by using a grinding instrument (MM 400, Retsch, Haan, Germany), and 50-mg tissue aliquots were extracted with 1.0 mL 70% aqueous methanol containing 0.1 mg/L lidocaine for watersoluble metabolites at 4°C overnight and vortexed three times. The extracts were clarified by centrifugation, combined, evaporated, and then filtered through 0.45-mm nylon membranes (SCAA 104; ANPEL http://www.…”