A combined HPLC‐UV and HPLC‐MS method for the identification of lignans and its application to the lignans of Linum usitatissimum L. and L. bienne Mill.

Abstract

A combined HPLC-UV/PAD and HPLC-ESI/MS method allowing the fast detection and identification/structural characterisation of lignans of different structural subclasses is described. Twenty-four lignans of different skeletal types were analysed and the combined information derived from their UV and ESI/MS spectra led to the identification of group characteristics that can be used to establish the structure of unknown lignans in plant samples. This method was successfully applied to the identification of lignans in crude extracts of Linum usitatissimum L. and L. bienne Mill.

Next Generation Sequencing in Predicting Gene Function in Podophyllotoxin Biosynthesis

“…From the three possible products formed by a methylenedioxy bridge-forming enzyme (either with formation of one methylenedioxy bridge in either one of the aromatic rings or in both), the expected biosynthetic pathway reaction product to (Ϫ)-podophyllotoxin (1b) would be (Ϫ)-pluviatolide (14b) rather than (Ϫ)-haplomyrfolin (40b). In this respect, the fragmentation pattern of the enzymatic product was consistent to that described in the literature (61,62) for (Ϫ)-pluviatolide (14b) (Fig. 7) ϩ (calculated 379.1157), respectively.…”

Development of a high-throughput LC/APCI-MS method for the determination of thirteen phytoestrogens including gut microbial metabolites in human urine and serum

“…Pseudomolecular ions were predominant, with [M+H] + for the positive and [M−H] − for the negative mode. However, for SECO, the fragment ion [M+H−2H 2 O] + was found to constitute the base peak (i.e., 100% relative intensity of the ion), in agreement with the observations by BambagiottiAlberti et al [29] and Schmidt et al [30]. Formation of this fragment ion is probably due to protonation of the ␥-hydroxyl groups of SECO and subsequent loss of water from the unstable oxonium ions.…”

Separation and quantification of lignans in Phyllanthus species by a simple chiral densitometric method

“…Lignans, representing a class of bioactive molecules in a large number of medicinal plants, are considered as an interesting source for lead structures toward new drugs and of interest as drugs in the area of cancer chemotherapy [1]. Their chemistry and biological activity as well as therapeutic potential have been reviewed by many workers [2 -5].…”