While laccases, multi-copper glycoprotein oxidases, are often able to catalyze oxidation of a broad range of substrates, such as phenols and amines in vitro, their precise physiological/biochemical roles in higher plants remain largely unclear, e.g., Arabidopsis thaliana contains 17 laccases with only 1 having a known physiological function. To begin to explore their roles in planta, spatial and temporal expression patterns of Arabidopsis laccases were compared and contrasted in different tissues at various development stages using RT-PCR and promoter-GUS fusions. Various cell-specific expressions were noted where specific laccases were uniquely expressed, such as LAC4 in interfascicular fibers and seed coat columella, LAC7 in hydathodes and root hairs, LAC8 in pollen grains and phloem, and LAC15 in seed coat cell walls. Such specific cell-type expression patterns provide new leads and/or strategies into determining their precise physiological/biochemical roles. In addition, there was an apparent redundancy of gene expression patterns for several laccases across a wide variety of tissues, lignified and non-lignified, perhaps indicative of overlapping function(s). Preliminary evidence, based on bioinformatics analyses, suggests that most laccases may also be tightly regulated at both transcriptional (antisense transcripts, histone and DNA methylation) and posttranscriptional (microRNAs) levels of gene expression.
This comprehensive review describes the current status and knowledge of biochemical and molecular processes involved in allyl/propenyl phenol, lignan, norlignan and lignin biosynthesis. Recent advances made over the last decade are critically discussed, and placed in context with earlier studies largely dating back to the 1950s. Beginning with the recently established formation of phenylalanine in plants, each downstream biochemical conversion is described from the perspective of the mechanistic details known to this point. Particular emphasis is placed upon proteinaceous control of monolignol-derived radical-radical coupling processes, leading to lignans and lignins, as well as apparently related processes affording the various ellagitannins and phenolic terpenoids. The evidence for non-random macromolecular lignin assembly is discussed in detail, this being in contrast to earlier notions that such processes were random. The latter assumptions have largely resulted from a lack of robust analytical procedures and rigorous quantification, as well as a lack of incisive experimental design. In addition, the often-noted severe effects of modulating lignin compositions and contents on plant vascular tissue properties (i.e. in terms of compromised biophysical properties) are described herein, as well as the severe limitations as regards recent claims of compensatory 'combinatorial chemistry' lignin formation. Much of the latter confusion has also resulted from the serious deficiencies in current lignin analytical protocols and quantification, as well as in the general lack of experimental approaches/design to probe lignin primary structure(s).
Background: How vascular plants control phenoxy radical coupling is enigmatic. Results: Two dirigents engendered (Ϫ)-pinoresinol formation in Arabidopsis. Coupling stereoselectivity was reversed from (ϩ)-to (Ϫ)-pinoresinol through swapping identical regions. Conclusion: Novel insights into stereoselective control over phenoxy radical coupling were obtained. Significance: This is the first report of dirigent-mediated phenoxy radical coupling control leading to opposite stereoselectivities and identification of protein regions involved.
Background: Biosynthetic pathways to structurally complex plant medicinals are incomplete or unknown. Results: Next generation sequencing/bioinformatics and metabolomics analysis of Podophyllum tissues gave putative unknown genes in podophyllotoxin biosynthesis. Conclusion: Regio-specific methylenedioxy bridge-forming CyP450s were identified catalyzing pluviatolide formation. Significance: Database of several medicinal plant transcriptome assemblies and metabolic profiling are made available for scientific community.
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