A combination of elicitor and precursor enhances psoralen production in Psoralea corylifolia Linn. suspension cultures

Gajula, Hari; Kumar, Vadlapudi; Vijendra, Poornima D.; Rajashekar, J.; Sannabommaji, Torankumar; Basappa, Giridhara

doi:10.1016/j.indcrop.2018.08.050

Cited by 24 publications

(8 citation statements)

References 34 publications

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“…SA is also known to induce reactive oxygen species (ROS) production as a part of the defense system in plants. Numerous studies displayed the exogenous effects of SA on the elicitation of different classes of phenolic compounds [15][16][17][18]. Remarkably, chitosan (CS) is non-toxic, cost-effective and the second most abundant polycationic biopolymer that is well documented in the elicitation of in vitro production of high-valued secondary metabolites such as aromatic amino acids, phenylpropanoids, plumbagin, tannins, triterpenoids, xanthones, secoiridoids etc.…”

Section: Introductionmentioning

confidence: 99%

Optimization of salicylic acid and chitosan treatment for bitter secoiridoid and xanthone glycosides production in shoot cultures of Swertia paniculata using response surface methodology and artificial neural network

et al. 2020

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Background: In this study, response surface methodology (RSM) and artificial neural network (ANN) was used to construct the predicted models of linear, quadratic and interactive effects of two independent variables viz. salicylic acid (SA) and chitosan (CS) for the production of amarogentin (I), swertiamarin (II) and mangiferin (III) from shoot cultures of Swertia paniculata Wall. These compounds are the major therapeutic metabolites in the Swertia plant, which have significant role and demand in the pharmaceutical industries. Results: Present study highlighted that different concentrations of SA and CS elicitors substantially influenced the % yield of (I), (II) and (III) compounds in the shoot culture established on modified ½ MS medium (supplemented with 2.22 mM each of BA and KN and 2.54 mM NAA). In RSM, different response variables with linear, quadratic and 2 way interaction model were computed with five-factor-three level full factorial CCD. In ANN modelling, 13 runs of CCD matrix was divided into 3 subsets, with approximate 8:1:1 ratios to train, validate and test. The optimal enhancement of (I) (0.435%), (II) (4.987%) and (III) (4.357%) production was achieved in 14 days treatment in shoot cultures of S. paniculata elicited by 9 mM and 12 mg L − 1 concentrations (SA) and (CS). Conclusions: In optimization study, (I) show 0.170-0.435%; (II) display 1.020-4.987% and (III) upto 2.550-4.357% disparity with varied range of SA (1-20 mM) and CS (1-20 mg L − 1). Overall, optimization of elicitors to promote secoiridoid and xanthone glycoside production with ANN modeling (r 2 = 100%) offered more significant results as compared to RSM (r 2 = 99.8%).

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Section: Introductionmentioning

confidence: 99%

Optimization of salicylic acid and chitosan treatment for bitter secoiridoid and xanthone glycosides production in shoot cultures of Swertia paniculata using response surface methodology and artificial neural network

et al. 2020

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“…Numerous studies have identified 100 μmol L −1 MeJA as an optimum concentration to enhance the biosynthesis of bioactive compounds such as psoralen, valtrate and phenols. [52][53][54] The positive effects of MeJA on the increase in secondary metabolites production have been confirmed in many studiesfor example psoralen production in suspension cultures of Psoralea corylifolia L., 52 production of phenolic acids in in vitro regenerated shoots of Salvia virgata Jacq., 55 gymnemic acid production in suspension cultures of Gymnema sylvestre L., 56 production of phenolic acids in HR cultures of Salvia miltiorrhiza, 22 phenolic metabolism in HR cultures of Daucus carota L., 57 and rosmarinic acid accumulation in HR cultures of Coleus blumei. 58 As the antioxidant capacity of plants is closely associated with their secondary metabolites, 59 MeJA could strongly improve the DPPH and FRAP antioxidant capacity of the elicited HRs depending on the concentration and exposure time.…”

Section: A1 A2 B M C + H 1 H 2 H 3 H 4 C -C º M C + H 1 H 2 C º C -M Hmentioning

confidence: 79%

“…Based on our results, although 100 and 200 μmol L −1 MeJA was effective for the highest production of TPC and TFC in Siah HR cultures, elicitation with 300 μmol L −1 of MeJA in addition to inducing the highest value of TFC in Sabz HR cultures, resulted in the highest amount of quercetin, apigenin, and cinnamic acid in both Sabz and Siah HRs and also caffeic acid and coumaric acid in Siah HR cultures. Numerous studies have identified 100 μmol L −1 MeJA as an optimum concentration to enhance the biosynthesis of bioactive compounds such as psoralen, valtrate and phenols . The positive effects of MeJA on the increase in secondary metabolites production have been confirmed in many studies – for example psoralen production in suspension cultures of Psoralea corylifolia L., production of phenolic acids in in vitro regenerated shoots of Salvia virgata Jacq., gymnemic acid production in suspension cultures of Gymnema sylvestre L., production of phenolic acids in HR cultures of Salvia miltiorrhiza , phenolic metabolism in HR cultures of Daucus carota L., and rosmarinic acid accumulation in HR cultures of Coleus blumei …”

Section: Discussionmentioning

confidence: 94%

Agrobacterium rhizogenes mediated transformation of Ficus carica L. for the efficient production of secondary metabolites

et al. 2020

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BACKGROUND Ficus carica L., an ancient source of food and medicines, is rich in valuable nutritional and secondary compounds with antioxidant, antimicrobial, and anticancer effects. The present study is the first attempt to examine hairy root (HR) induction of F. carica (Sabz and Siah) by inoculating the 3‐week‐old shoots and leaves with different strains of Agrobacterium rhizogenes and also to investigate methyl jasmonate (MeJA) elicitation of HRs to produce a fast and high‐yield production method for secondary metabolites. RESULTS The maximum transformation rate (100%) was achieved by inoculating the shoots with Agrobacterium rhizogenes strain A7. Siah HRs elicited with 100 and 200 μmol L−1 MeJA and Sabz HRs with 100 μmol L−1 MeJA showed the highest total phenolic content. The highest flavonoid content was 3.935 mg QE g−1 DW in Siah HRs treated with 200 μmol L−1 MeJA and 2.762 mg QE g−1 DW in Sabz HRs treated with 300 μmol L−1 MeJA. The 2, 2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging capacity and ferric reducing antioxidant power (FRAP) value of HRs were affected by MeJA treatments. Methyl jasmonate elicitation also significantly enhanced the content of six phenolic acids (gallic acid, caffeic acid, chlorogenic acid, coumaric acid, rosmarinic acid, and cinnamic acid) and three flavonoids (rutin, quercetin, and apigenin). Thymol, a monoterpene phenol, was the main HR compound detected in gas chromatography mass spectrometry (GC–MS) analysis of the essential oils. CONCLUSION Induction of HRs and elicitation of F. carica HRs by MeJA resulted in a significant increase in the production of important phenolic compounds and a significant increase in antioxidant capacity. © 2020 Society of Chemical Industry

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“…Mendoza et al [28] reported that elicitation with 3 µM MeJa increased phenolics and avonoids content in Thevetia peruviana suspension cultures. The production of psoralen was enhanced when MeJa and SA at 100 µM were added to the suspension culture of Psoralea corylifolia [42]. Therefore, MeJa has been well established as a key signal compound which was positively involved in the signal transduction pathway that leads to secondary metabolite production [43,35].…”

Section: Discussionmentioning

confidence: 99%

Enhanced in vitro production of diosgenin in shoot cultures of Dioscorea deltoidea by elicitation and precursor feeding

Nazir

Gupta

Kumar

et al. 2020

Preprint

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Background: Dioscorea deltoidea (Family: Dioscoreaceae) is a critically endangered medicinal plant widely used in traditional medicine and pharmaceutical industries for the preparation of appropriate drugs. The present study was conducted to investigate the effect of different elicitors such as salicylic acid (SA), methyl jasmonate (MeJa), jasmonic acid (JA) and abscisic acid (ABA) on the synthesis of diosgenin production in D. deltoidea shoot cultures. In addition, the effect of different precursors (squalene, β-sitosterol, and cholesterol) was also demonstrated. Results: Results showed that precursors significantly influenced diosgenin production as compared to elicitors. Application of SA (200 µM) showed highest diosgenin production (0.912% DW) following 4 h of incubation time, whereas MeJA (100 µM) exhibited 0.814% DW of diosgenin content following 8 h of incubation time. Among precursors, β-sitosterol at 200 µM produced maximum diosgenin content (1.006% DW) followed by 100 µM squalene (0.947% DW) harvested after 5th day of the precursor addition. Interestingly, cholesterol showed low diosgenin production, but significant than control cultures. Finally response surface methodology (RSM) was conducted to see the interactive effect of best elicitors and precursors concentration for better yield of diosgenin and biomass.Conclusions: The results revealed that exposure to different elicitors and precursors have a significant influence on accumulation of diosgenin in D. deltoidea shoot cultures. The optimized conditions for SA and β-sitosterol for the better yield of diosgenin and biomass were found to be 165 µM, 150 µM and 170 µM, 140 µM respectively.

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A combination of elicitor and precursor enhances psoralen production in Psoralea corylifolia Linn. suspension cultures

Cited by 24 publications

References 34 publications

Optimization of salicylic acid and chitosan treatment for bitter secoiridoid and xanthone glycosides production in shoot cultures of Swertia paniculata using response surface methodology and artificial neural network

Optimization of salicylic acid and chitosan treatment for bitter secoiridoid and xanthone glycosides production in shoot cultures of Swertia paniculata using response surface methodology and artificial neural network

Agrobacterium rhizogenes mediated transformation of Ficus carica L. for the efficient production of secondary metabolites

Enhanced in vitro production of diosgenin in shoot cultures of Dioscorea deltoidea by elicitation and precursor feeding

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