A colorimetric indicator-displacement assay based on stable Cu2+ selective carbon dots for fluorescence turn-on detection of pyrophosphate anions in urine

Yang, Yi; Chen, Pei; Liu, Yuqing; Cai, Zheng; Wang, Xiyao; Me, Yang; Ding, Xiongyu; Lin, Lan; Jiang, Huijun; Zhang, Zhenqin; Ju, Yichun

doi:10.1016/j.saa.2021.119479

Cited by 18 publications

(3 citation statements)

References 46 publications

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“…The PPi level in urine is associated with urolithiasis and kidney-related diseases. The concentration range of PPi in normal human urine is 1.8 × 10 −5 –11 × 10 −5 M [ 32 , 33 , 34 ]. The AuNC-Cu 2+ sensing system was applied for determination of PPi in human urine according to the procedures described in Section 2 .…”

Section: Resultsmentioning

confidence: 99%

Copper (II) Ion-Modified Gold Nanoclusters as Peroxidase Mimetics for the Colorimetric Detection of Pyrophosphate

Shi

Wang

et al. 2021

Sensors

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Copper (II) ions have been shown to greatly improve the chemical stability and peroxidase-like activity of gold nanoclusters (AuNCs). Since the affinity between Cu2+ and pyrophosphate (PPi) is higher than that between Cu2+ and AuNCs, the catalytic activity of AuNCs-Cu2+ decreases with the introduction of PPi. Based on this principle, a new colorimetric detection method of PPi with high sensitivity and selectivity was developed by using AuNCs-Cu2+ as a probe. Under optimized conditions, the detection limit of PPi was 0.49 nM with a linear range of 0.51 to 30,000 nM. The sensitivity of the method was three orders of magnitude higher than that of a fluorescence method using AuNCs-Cu2+ as the probe. Finally, the AuNCs-Cu2+ system was successfully applied to directly determine the concentration of PPi in human urine samples.

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Section: Resultsmentioning

confidence: 99%

Copper (II) Ion-Modified Gold Nanoclusters as Peroxidase Mimetics for the Colorimetric Detection of Pyrophosphate

Shi

Wang

et al. 2021

Sensors

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“…At present, the uorescent sensing materials constructed by metal ions and uorescent small molecules have been applied in many elds [19,20]. These complex uorescent sensing systems are usually based on an on-off or off-on detection strategy.…”

Section: Introductionmentioning

confidence: 99%

A Poly(carbazole-alt-triazole) with Thiabendazole Side Groups as an “On-Off-On” Fluorescent Probe for Detection of Cu(II) Ion and Cysteine

Gao

Luo

et al. 2023

J Fluoresc

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A novel conjugated polymer PCZBTA-TBZ containing thiabendazole as recognition unit was synthesized via Suzuki coupling reaction, and its structural characterization, spectroscopic analysis and photophysical properties were investigated. In the metal ion response study, the addition of Cu 2+ led to the occurrence of the PET mechanism, which signi cantly quenched the uorescence of the polymer PCZBTA-TBZ with a quenching effect of 98%. According to the density functional theory calculation, compared with other polycarbazoles or other alternative copolymers containing carbazole, with alternating carbazole and triazole enhances the electron mobility and reduces the energy band gap of the polymer. Due to the strong coordination ability between Cu 2+ and Cys, the adding Cys competes the Cu 2+ in the [PCZBTA-TBZ-Cu 2+ ] complex, blocking the occurrence of PET, and the uorescence intensity of PCZBTA-TBZ is restored. The addition of other amino acids caused almost no change. The polymer is expected to be used for dual uorescence detection of speci c metal ions and Cys.

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“…Recently, it was demonstrated that an accumulation of Cu 2+ affects the activity of dopaminergic neurotransmitters, potentially leading to neurodegenerative disorders and mental issues, including anxiety, depression, language, and cognitive impairment [ 5 , 6 , 7 ]. In this context, a few fluorescent methods were developed for Cu 2+ quantitative analysis in urine [ 8 , 9 , 10 , 11 , 12 , 13 , 14 ]. In this study, for the first time, the fluorescent properties of a novel fluorophore derived from serotonin (SE) self-oxidation and polymerization were exploited for sensitive Cu 2+ detection in human urine without matrix pretreatment.…”

Section: Introductionmentioning

confidence: 99%

Serotonin-Derived Fluorophore: A Novel Fluorescent Biomaterial for Copper Detection in Urine

Lettieri

Scarano

Caponi

et al. 2023

Sensors

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We took advantage of the fluorescent features of a serotonin-derived fluorophore to develop a simple and low-cost assay for copper in urine. The quenching-based fluorescence assay linearly responds within the concentration range of clinical interest in buffer and in artificial urine, showing very good reproducibility (CVav% = 4% and 3%) and low detection limits (16 ± 1 μg L−1 and 23 ± 1 μg L−1). The Cu2+ content was also estimated in human urine samples, showing excellent analytical performances (CVav% = 1%), with a limit of detection of 59 ± 3 μg L−1 and a limit of quantification of 97 ± 11 μg L−1, which are below the reference value for a pathological Cu2+ concentration. The assay was successfully validated through mass spectrometry measurements. To the best of our knowledge, this is the first example of copper ion detection exploiting the fluorescence quenching of a biopolymer, offering a potential diagnostic tool for copper-dependent diseases.

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A colorimetric indicator-displacement assay based on stable Cu2+ selective carbon dots for fluorescence turn-on detection of pyrophosphate anions in urine

Cited by 18 publications

References 46 publications

Copper (II) Ion-Modified Gold Nanoclusters as Peroxidase Mimetics for the Colorimetric Detection of Pyrophosphate

Copper (II) Ion-Modified Gold Nanoclusters as Peroxidase Mimetics for the Colorimetric Detection of Pyrophosphate

A Poly(carbazole-alt-triazole) with Thiabendazole Side Groups as an “On-Off-On” Fluorescent Probe for Detection of Cu(II) Ion and Cysteine

Serotonin-Derived Fluorophore: A Novel Fluorescent Biomaterial for Copper Detection in Urine

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