A collinear light-emitting diode-induced fluorescence detector for capillary electrophoresis

Yang, Bingcheng; Tan, Feng; Guan, Yafeng

doi:10.1016/j.talanta.2004.09.012

Cited by 22 publications

(15 citation statements)

References 21 publications

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“…Recently, LED induced fluorescence detector has aroused much interest [18][19][20][21][22] because of its low cost, simplicity and flexibility. The LED induced fluorescence detection systems in the previous CE studies [23,24] suffer from the light reflecting and scattering on capillary surface, for which that the excitation light excited analytes from outside of the capillary at the detection window, which could result in high background noise and low detection sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Determination of excitatory amino acids in biological fluids by capillary electrophoresis with optical fiber light-emitting diode induced fluorescence detection

Wang

Zhao

Yuan

et al. 2006

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Determination of excitatory amino acids in biological fluids by capillary electrophoresis with optical fiber light-emitting diode induced fluorescence detection

Wang

Zhao

Yuan

et al. 2006

Journal of Chromatography B

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“…The combination of high stability, small size, low cost, and very long lifetime make LEDs an very attractive excitation source for fluorescence detection, especially to design miniaturized or portable devices [80, 81]. Using this approach, Arraez-Roman and coworkers constructed a simple fluorescence detector for both direct and indirect CE methods using a blue LED (470 nm, LED-470, Ocean Optics) as excitation source, a bifurcated optical fiber as a waveguide, and a CCD camera as a detector [82].…”

Section: - Instrumentationmentioning

confidence: 99%

Recent developments in instrumentation for capillary electrophoresis and microchip‐capillary electrophoresis

2010

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Over the last years there has been an explosion in the number of developments and applications of capillary electrophoresis (CE) and microchip-CE. In part, this growth has been the direct consequence of recent developments in instrumentation associated with CE. This review, which is focused on contributions published in the last five years, is intended to complement the papers presented in this special issue dedicated to Instrumentation and to provide an overview on the general trend and some of the most remarkable developments published in the areas of high voltage power supplies, detectors, auxiliary components, and compact systems. It also includes few examples of alternative uses of and modifications to traditional CE instruments.

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“…Detection limits in the nM range are obtained, which are comparable or better than LED based multi-wavelength systems previously reported. While no reports could be found on the use of CBQCA and NBD-F by CE using LEDIF, considerable work has been done with FITC derivatised amines, with LODs between 5 and 12 nM [16,23,24].…”

Section: Comparison Of Multi-led and Single-led Excitationmentioning

confidence: 99%

Multi‐wavelength light emitting diode array as an excitation source for light emitting diode‐induced fluorescence detection in capillary electrophoresis

et al. 2010

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A multi-wavelength LED array was used as an excitation source for in-column fiber-optic LED-induced fluorescence for CE. The light source consisted of a multi-wavelength LED array consisting of three different LEDs (430, 450 and 480 nm), a focusing lens and a gradient index lens group. The LED beam was collimated and reshaped with the gradient index lens group for coupling the LED light source into a single-mode optical fiber. In addition, the luminance and stability of the LED light source was improved by powering the LED under constant current at enhanced voltages. The benefits of this system were demonstrated by the simultaneous determination of FITC-labeled L-asparagine (Ex/Em 488/520 nm), 4-fluoro-7-nitro-2,1,3-benzoxadiazole-labeled epinephrine (Ex/Em 468/530 nm) and 3-(4-carboxybenzoyl)-quinoline-2-carboxaldehyde-labeled L-leucine (Ex/Em 440/530 nm). Detection limits of L-asparagine, epinephrine and L-leucine were estimated to be 0.8 x 10(-9), 12.0 x 10(-8) and 4.0 x 10(-8) M (S/N=3), respectively. The RSDs (n=6) for migration time and peak area were better than 0.71 and 0.92%, respectively. The performance of the developed multi-wavelength LED excitation source was compared to the use of a single-wavelength LED and found to provide superior sensitivity for the three fluorophores used in this study.

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A collinear light-emitting diode-induced fluorescence detector for capillary electrophoresis

Cited by 22 publications

References 21 publications

Determination of excitatory amino acids in biological fluids by capillary electrophoresis with optical fiber light-emitting diode induced fluorescence detection

Determination of excitatory amino acids in biological fluids by capillary electrophoresis with optical fiber light-emitting diode induced fluorescence detection

Recent developments in instrumentation for capillary electrophoresis and microchip‐capillary electrophoresis

Multi‐wavelength light emitting diode array as an excitation source for light emitting diode‐induced fluorescence detection in capillary electrophoresis

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