A clinical study on the expression of PTEN in renal cell carcinoma in children

Abstract

The expression pattern of tumor suppressor gene phosphatase and tensin homolog deleted on chromosome ten (PTEN) and phosphatase and tensin homolog deleted on chromosome ten/phosphatidylinositol3-kinase/protein kinase B (PTEN/PI3K/AKT) cell signaling pathway in renal cell carcinoma (RCC) were investigated in children. A total of 5 cases of RCC (observation group) in children and 10 cases of benign kidney tumor (control group) diagnosed by pathological examinations were included to obtain tumor samples. Expression of PTEN mRNA was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The protein expression of PTEN, PI3K and AKT was detected by western blotting; relationships between the expression level of PTEN mRNA and the clinical features of RCC were analyzed. It turned out that expression level of PTEN mRNA in the observation group was significantly lower than that in the control group. The protein expression levels of PTEN, PI3K and AKT were significantly lower in the observation group than in the control group (P<0.05). The expression level of PTEN mRNA decreased with the increased clinical stage of RCC (P<0.05), and was not related to sex, age and maximum tumor diameter (P>0.05). The results showed that downregulation of the tumor suppressor gene PTEN expression and the inhibition of PTEN/PI3K/AKT cell signaling pathway may be involved in the occurrence and development of RCC in children.

Research Progress of PI3K/PTEN/AKT Signaling Pathway Associated with Renal Cell Carcinoma

“…A recent study has revealed that the activation of the PI3K/PTEN/AKT signaling pathway inhibits the induction of apoptosis in tumor cells by exogenous stimuli, which consequently promotes cell proliferation and metastasis [ 8 ]. Moreover, it has been suggested that blocking the activation of its effector molecules by inhibiting the PI3K/PTEN/AKT signaling pathway could potentially serve as a new idea for the treatment of renal cell carcinoma [ 9 ]. Accordingly, this study reviews the progress of PI3K/PTEN/AKT signaling pathway research in renal cell carcinoma.…”

lncRNA F11-AS1 Suppresses Glioma by Regulation miRNA-3146/PTEN

“…PTEN is intervened by gene or small-molecule inhibitor. Some good laboratory results on the inhibition of the proliferation of tumor cells by P13K/AKT pathway have been obtained [18][19][20][21][22][23]. The results of this study proved that the overexpression of F11-AS1 causes the decrease of the expression of miRNA-3146, so as to inhibit the PI3K/AKT signaling pathway after the activation of PTEN and inhibit the bioactivity of glioma cells.…”