A chiral ligand exchange CE system for monitoring inhibitory effect of kojic acid on tyrosinase

Sun, Bo; Qi, Li; Mu, Xiaoyu; Qiao, Juan; Wang, Minglin

doi:10.1016/j.talanta.2013.08.028

Cited by 22 publications

(17 citation statements)

References 33 publications

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“…Sun et al. developed also a CLE‐CE method with Zn (II)‐L‐alanine as the chiral selector in the presence of β‐CD for enantioseparation of dansylated‐AAs . CLE‐CE‐UV method was used to explore the inhibitory effect of kojic acid and soy sauce on the enzyme tyrosinase.…”

Section: Chiral Analysis Of Food Compoundsmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2015

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This review work presents and discusses the main applications of capillary electromigration methods in food analysis and Foodomics. Papers that were published during the period February 2013-February 2015 are included following the previous review by Garcia-Cañas et al. (Electrophoresis, 2014, 35, 147-169). Analysis by CE of a large variety of food-related molecules with different chemical properties, including amino acids, hazardous amines, peptides, proteins, phenols, polyphenols, lipids, carbohydrates, DNAs, vitamins, toxins, contaminants, pesticides, residues, food additives, as well as small organic and inorganic compounds. This work includes recent results on food quality and safety, nutritional value, storage, bioactivity, as well as applications of CE for monitoring food processing. The use, among other CE developments, of microchips, CE-MS, and chiral CE in food analysis and Foodomics is also discussed.

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Section: Chiral Analysis Of Food Compoundsmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2015

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“…When using substrates without UV chromophores, derivatization of the products and/or substrates can be performed. This has been realized for amino acid-derived substrates by derivatization with dabsyl chloride [28,29], dansyl chloride [30][31][32], fluorenylmethyloxycarbonyl (Fmoc) chloride [33] or phenylisothiocyanate [34]. Carbohydrate substrates were derivatized by reductive amination with 7-aminonaphthalene-1,3-disulfonic acid [35].…”

Section: Pre-capillary Enzyme Assaysmentioning

confidence: 99%

Advances in Capillary Electrophoresis-Based Enzyme Assays

Scriba

Belal

2015

Chromatographia

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IntroductionEnzymes catalyze metabolic reactions in cells regulating homeostasis, growth and reproduction of living organisms. Enzymes are also often involved in human disease. The analysis of the human genome has revealed that within the so-called druggable genome a significant portion (in some analyses more than half) of the potential drug targets are enzymes [1][2][3]. Consequently, enzymes are important targets in the development of new drugs. Moreover, enzymes play a role in the clinical diagnosis of diseases. Therefore, effective methods for characterization of enzymes as well as for the determination of their activity are important, for example, for the understanding of enzyme-mediated metabolic reactions or the identification of substrates and inhibitors for the treatment of human diseases.Capillary electrophoresis (CE) has been applied to enzyme analysis for more than 20 years since the first report by Banke et al. on an assay of alkaline protease [4]. Since then, all aspects of enzyme-related analysis have been studied by CE methods including the evaluation of enzymatic activity, enzyme kinetics, identification and characterization of enzyme inhibitors and activators as well as metabolic pathways as, for example, summarized in [5][6][7][8][9][10][11][12].Generally, CE-based enzyme assays can be divided in three groups, pre-capillary (offline) assays, in-capillary (online) assays and post-capillary assays. In the pre-capillary assays, all required components including enzyme, substrate, co-factors, etc., are mixed in a vial and incubated for an intended period of time. Subsequently, the reaction is quenched and an aliquot is subjected to CE analysis for the determination of substrate(s) and/or co-substrate(s) and/ or product(s). Multiple sampling or repeated sampling in combination with sequential runs for the determination of Abstract Capillary electrophoresis (CE) has become a flexible and accurate, high-efficiency analytical separation technique in many areas requiring only minute amounts of sample and chemicals. Thus, CE has also been recognized as a suitable technique to study enzymatic reactions including the determination of Michaelis-Menten kinetic data or the identification and characterization of inhibitors. The most often applied CE-based enzyme assay modes can be divided into two categories: (1) pre-capillary assays where incubations are performed offline followed by CE analysis of substrate(s) and/or product(s) and (2) in-capillary assays in which the enzymatic reaction and analyte separation are performed in the same capillary. In case of the in-capillary assays, the enzyme may be immobilized or in solution. The latter is also referred to as electrophoretically mediated microanalysis (EMMA), while in the case of immobilized enzyme the term immobilized enzyme reactor (IMER) is used. The present review summarizes the literature on CEbased enzyme assays published between January 2010 and April 2015. Immobilized enzyme reactors as well as microfluidic devices applied to the study of enzymatic a...

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“…In another work, dansylated DL‐Tyr has been separated with Zn 2+ and L‐Ala with β‐cyclodextrin. The activity of tyrosinase and its inhibitor (kojic acid and soy sauce) has been investigated off‐line .…”

Section: Separation Of Enantiomersmentioning

confidence: 99%

Recent advances in amino acid analysis by capillary electromigration methods, 2013–2015

et al. 2015

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We describe the most important research articles published on amino acid analysis using CE during the period from June 2013 to May 2015, and follows the format of the previous articles published in electrophoresis the new developments in amino acid analysis with CE are mainly describing improvements in detection means and injection methods. Enantiomeric separation developments are still important. Focusing the applications, we describe the neurochemical and clinical works, but also the metabolomic studies for which the publication number increase greatly. Finally, works focused on amino acids in food and agricultural applications are described.

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A chiral ligand exchange CE system for monitoring inhibitory effect of kojic acid on tyrosinase

Cited by 22 publications

References 33 publications

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

Advances in Capillary Electrophoresis-Based Enzyme Assays

Recent advances in amino acid analysis by capillary electromigration methods, 2013–2015

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