A chemiluminescent metalloimmunoassay based on copper‐enhanced gold nanoparticles for quantification of human growth hormone

Abstract

A chemiluminescence (CL) immunoassay was developed to determine human growth hormone (hGH) based on copper-enhanced gold nanoparticles. In this method, gold nanoparticles were deposited on polystyrene wells for adsorption of human growth antibodies as well as catalyst for reducing of copper ions from the copper enhancer solution. The reduction of copper ions was prevented where the gold nanoparticles were covered by the antibody-antigen immunocomplex. The deposited copper on Au nanoparticles was then dissolved in HNO3 solution and quantified using the CL method. The CL intensity response was logarithmically dependent on the hGH concentrations over the range 0.2-50 ng/mL, with a detection limit (3σ) of 0.036 ng/mL.

Functional nanoprobes for ultrasensitive detection of biomolecules: an update

“…Metal deposition provides a relative easy and straightforward approach for signal amplification. [81][82][83] Au NPs are often employed as seeds for the deposition of gold, silver and copper. Non-metal nanomaterials, e.g.…”

“…91 By using copper-enhanced gold NPs, Khayamian and co-workers reported an enhanced chemiluminescent (CL) immunoassay for sensitive detection of human growth hormone (hGH). 81 Thaxton et al employed gold deposition to dramatically increase the light scattering properties of their SNA-Au NP probes. 92 By coupling gap-tailorable gold-silver core-shell nanodumbbells to SERS, Lim et al explored a signal amplified Raman sensing for imaging and sensing applications.…”

Nanoparticle-based immunoassays in the biomedical field

“…22 Hasanpour et al developed a CL metalloimmunoassay based on copper-enhanced gold nanoparticles for the quantication of human growth hormone. 23 Tu and colleagues used a CL method as the exciting light source to construct a universal photoelectrochemical platform based on reduced graphene oxide-CdS nanocomposites. 24 Experimental results indicated that the developed immunosensing strategy could greatly improve the photovoltaic transfer efficiency and result in excellent performance for the photoelectrochemical immunoassay (Fig.…”

Gold nanoparticle-enhanced luminol/ferricyanide chemiluminescence system for aristolochic acid-I detection in medicinal plants and slimming products

“…Due to its simplicity, sensitivity, accuracy and reliability in the determination of different substances, researchers have used it extensively, especially for nano-scale detection (20,21). Because of their chemical stability and high resistance to surface oxidation, gold nanoparticles (AuNPs) are one of the most attractive nanomaterials that can be used to catalyze CL reactions (22,23). Green analytical chemistry is defined as the collaboration of efforts to develop cleaner or more eco-friendly techniques characterized by eliminating or at least minimizing emission of pollutants into the environment without compromising efficiency.…”