Nanoparticle-based immunoassays in the biomedical field

Tang, Dianping; Cui, Yuling

doi:10.1039/c2an36500f

Cited by 99 publications

(51 citation statements)

References 95 publications

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“…More importantly, the function patterns serve as quality control (i.e., positive control) that ensures the normal state of the fluorescent antibody, just as the incorporation of the control line in widely used lateral flow test strips. [34][35][36][37] The normal state of the fluorescent antibody guarantees the integrity of the 2D barcode-like patterns, otherwise the array cannot be analyzed automatically because of the disappearance of the function patterns. The high performance of the automatic analysis directly resulted from the simple algorithms and the corresponding code, which was realized by our interdisciplinary approach combining biochemistry, microfabrication and mathematics in the development of this analytical device.…”

Section: A Efficiency Evaluationmentioning

confidence: 99%

Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays

et al. 2013

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The usability of many high-throughput lab-on-a-chip devices in point-of-care applications is currently limited by the manual data acquisition and analysis process, which are labor intensive and time consuming. Based on our original design in the biochemical reactions, we proposed here a universal approach to perform automatic, fast, and robust analysis for high-throughput array-based microfluidic immunoassays. Inspired by two-dimensional (2D) barcodes, we incorporated asymmetric function patterns into a microfluidic array. These function patterns provide quantitative information on the characteristic dimensions of the microfluidic array, as well as mark its orientation and origin of coordinates. We used a computer program to perform automatic analysis for a high-throughput antigen/antibody interaction experiment in 10 s, which was more than 500 times faster than conventional manual processing. Our method is broadly applicable to many other microchannel-based immunoassays. V C 2013 AIP Publishing LLC. [http://dx

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Section: A Efficiency Evaluationmentioning

confidence: 99%

Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays

et al. 2013

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“…It is used for solid phase immobilization (on the microtiter wells), horseradish peroxidase (HRP) the most commonly used ELISA enzymes which is desired antibody label due to it being the smallest and most stable [12]. The application of AuNPs and AgNPs to commercially available T3-competitive ELISA test can be useful to improve and modify ELISA method [16,17].…”

Section: Theoretical Conceptmentioning

confidence: 99%

Biochemical study for gold and silver nanoparticles on thyroid hormone levels in saliva of patients with chronic renal failure

Jassim

Al-Kazaz

2013

Eur. J. Chem.

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KEYWORDSNoble metal nanoparticles were synthesized directly by pulsed laser ablation (Nd:YAG, λ=1064 nm) of gold and silver plates immersed in pure water. Concentrations of the nanoparticles were determined by atomic absorption spectroscopy measurement. Atomic force microscope and transmission electron microscope analysis were used to characterize the size and size distributions of the metals nanoparticles. The objective of this work is studying the effects of presence gold and silver nanoparticles on the levels of triiodothyronine hormone (T3) in saliva of patients with chronic renal failure their thyroid had disorder (hypothyroidism). Also the study characterized the binding between the antiT3antibody with its antigen i.e., T3 in saliva patients in the presence affixed size concentration of nanoparticles to improve and modify a competitive ELISA method. It is found that both gold and silver nanoparticles demonstrated activation effect on the binding between anti-T3 antibody and antigen (T3) in the saliva of patients and these effects increased with increasing the concentrations and size for both nanoparticles (gold was more activation effect than silver). Optimization of experimental conditions for the binding in the saliva were: 40 μL and 20 μL of saliva (T3) in presence both silver and gold nanoparticle, respectively, pH = 7.4 of 0.2 M phosphate buffer was selected as the detection solution, the temperature of incubation was 22 °C while the incubation time were 30 min, 90 min for silver and gold nanoparticles, respectively.

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“…21,22 The resonance properties of AuNPs generate oscillation and scattering of conductive electrons. The resonance properties depend on the size, shape, and refractive index of AuNPs.…”

Section: Introductionmentioning

confidence: 99%

A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick

et al. 2018

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Gold nanoparticle (AuNP)-based optical assays are of significant interest since the molecular phenomenon can be examined easily with change in the color of AuNPs. Herein, we report the development of a dipstick using a AuNP-labeled single-chain fragment variable (scFv) antibody for the detection of morphine. The scFv antibodies for morphine were developed using phage display-based antibody library. Immunoglobulin variable regions of heavy (V H )-and light (V L )-chain genes were connected via a glycine-serine linker isolated from murine immune repertoire and cloned into the expression vector pIT2. The scFv was produced in Escherichia coli HB2151, yielding a functional protein with a molecular weight of approximately 32 kDa. The morphine scFv was labeled with gold nanoparticles and used as an optical immunoprobe in a dipstick. The competitive dipstick assay characterized the ability of the scFv antibody to recognize free morphine. The detection range was 1-1000 ng mL À1 with a limit of detection (LOD) of 5 ng mL À1 under optimal conditions, and the IC 50 value was 14 ng mL À1 for morphine. The developed optical dipstick kit of scFv antibody was capable of specifically binding to free morphine and its analogs in a solution in less than 5 min and could be useful for on-site screening of a real sample in blood, urine, and saliva.

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Nanoparticle-based immunoassays in the biomedical field

Cited by 99 publications

References 95 publications

Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays

Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays

Biochemical study for gold and silver nanoparticles on thyroid hormone levels in saliva of patients with chronic renal failure

A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick

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