A chemical method of gentamicin bonding to gelatine-sealed prosthetic vascular grafts

Ginalska, Grażyna; Kowalczuk, Dorota; Osińska, Monika

doi:10.1016/j.ijpharm.2004.09.021

Cited by 33 publications

(31 citation statements)

References 16 publications

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“…Silver, among well‐known antibacterial metals such as copper and zinc, captures great attentions because of its most powerful antimicrobial activity in low concentration (35 ppb)10, 17–18 besides no toxicity or low toxicity to mammalian cells 10, 19. All kinds of pathogenic organisms are killed in 3–4 min upon contact 20. Also, silver has anti‐inflammatory characteristic that quicken wound healing 21.…”

Section: Introductionmentioning

confidence: 99%

In vitro antibacterial evaluation of sol–gel‐derived Zn‐, Ag‐, and (Zn + Ag)‐doped hydroxyapatite coatings against methicillin‐resistant Staphylococcus aureus

Samani

Hossainalipour

Tamizifar

et al. 2012

J Biomedical Materials Res

117

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Hydroxyapatite (HAp) coatings were applied using sol-gel method. Phosphor pentoxide and calcium nitrate were used as phosphorous and calcium precursors, respectively. Zinc nitrate and silver nitrate were used as substitute of calcium in HAp structure. As a base concentration, 1.5 wt %Ag and 2.5 wt %Zn were used. The weight percent of Ag was increased at 0.3 wt% and Zn content was scaled down at 0.5 wt%. Phase analysis and chemical bonds of synthesized materials were studied by XRD and FTIR. Antibacterial activity of Ag- and Zn-doped samples against methicilin-resistant Staphylococcus aureus (MRSA) were assessed by the plate-counting method. The XRD and FTIR results proved formation of HAp compound. Colony counting showed that silver and zinc ions prevent proliferation and growth of MRSA. Interestingly, co-presence of metal ions improves the antibacterial effectiveness of the coatings and the combined effect was greater than sum of the individual effects when each was administered alone. Overall, synergism between antibacterial activities of Zn(2+) and Ag(+) ions against MRSA can be suggested. Thus, cell toxicity decreases and biocompatibility increases without any decrement in antibacterial activity.

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Section: Introductionmentioning

confidence: 99%

In vitro antibacterial evaluation of sol–gel‐derived Zn‐, Ag‐, and (Zn + Ag)‐doped hydroxyapatite coatings against methicillin‐resistant Staphylococcus aureus

Samani

Hossainalipour

Tamizifar

et al. 2012

J Biomedical Materials Res

117

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“…Gentamicin immobilization method, described in patent pending, was based on procedure of protein‐sealed matrix activation via bifunctional agent, with formation of aldehyde groups on the surface. Antibiotic was further coupled to prepared matrices via its free NH 2 groups, as described elsewhere 23. During the first 15 min, after antibiotic solution was added to the matrices (both controls and protein‐sealed matrices), the reduced pressure was applied to ensure that the air was removed from biomaterial structure.…”

Section: Methodsmentioning

confidence: 99%

“…Earlier research has shown the effective covalent immobilization of aminoglycoside antibiotics on protein‐coated silica supports and commercially available gelatin‐sealed vascular prostheses 22–25. The modified grafts gradually released the attached antibiotic and effectively inhibited growth of various reference and clinical bacterial strains for at least 30 days during in vitro tests.…”

Section: Introductionmentioning

confidence: 99%

Covalent coating of hydroxyapatite by keratin stabilizes gentamicin release

et al. 2008

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A novel hybrid hydroxyapatite (HAP) matrix, covalently coated with rarely applied, hardly degradable keratin and effectively modified by gentamicin immobilized in mixed-type mode (via interactions of diverse strength), was created. This hybrid showed a remarkably high drug immobilization yield and the most sustainable antibiotic release among all tested composites. It was also able to inhibit bacterial growth, both in surrounding liquid and on matrix surface, much longer (for at least 121 days of experiment) than analogous gelatin-modified and nonmodified matrices. Gentamicin-keratin-coated-HAP granules were nontoxic to human osteoblasts and enabled their proliferation with a rate similar as noncoated HAP. Presence of keratin on HAP granules seemed to slightly enhance the osteoblast proliferation. The results indicate that newly created HAP hybrid with covalently immobilized keratin and gentamicin--nontoxic and osteoblast-friendly--is a promising biomaterial of significantly prolonged antibacterial activity.

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“…What's more, gelatin can be used as the carrier of antibiotics [22,23]. Gentamicin sulphate (GS) is an aminoglycoside broad-spectrum antibiotic used against bacterial infections through inhibits the bacteria protein synthesis [24]. Moreover, the bacterial resistance of GS is lower than other aminoglycosides.…”

Section: Accepted Manuscriptmentioning

confidence: 99%