A cell-factory model of Saccharomyces cerevisiae based on bacterial cellulose without GMO for consolidated bioprocessing of starch

Drosos, Athanasios; Boura, Konstantina; Dima, Agapi; Soupioni, M.; Nigam, Poonam Singh Nee; Κουτίνας, Αθανάσιος Α.

doi:10.1016/j.fbp.2021.05.006

Cited by 15 publications

(14 citation statements)

References 36 publications

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“…The presence of the band at 1546 cm − 1 at water-treated pellicles compared to alkali-treated pellicles, corresponded to the amide bond and are associated with proteins and residual biomass that are biological impurities likely originated from the bacteria or compounds in the culture medium that may have remained attached to the cellulose layers (George et al 2008). In our previous study, this band appeared in the water-treated BCS (without chemical treatment) and was used successfully for the BC-cell-factory preparation, which was used for the consolidated bioprocessing of starch to ethanol (Drosos et al 2021). The appearance of a several peaks at 1450 − 1300 cm − 1 was further supported the presence of the CH (stretching and bending vibrations of CH group of glucose units).…”

Section: Thermal Propertiesmentioning

confidence: 71%

“…The stock culture medium of the K. sucrofermentans strain DSM (Deutsche Sammlung von Mikroorganismen) 15973 T starter culture was prepared based on the method that was reported by Drosos et al (Drosos et al 2021).…”

Section: Methodsmentioning

confidence: 99%

“…For the solid inoculum preparation, as reported in the method by Drosos et al (Drosos et al 2021), 10% v/v (10 6 cells/mL) of the starter culture was combined with 20 mL fresh Hestrin and Schramm (HS) medium in 50 mL asks and new cultures allowed to grow for 4 days at 30°C to result in the formation of the rst cellulose layer (pellicle) on the top surface of the culture medium. The initial bacterial pellicles were used as the solid inoculum of K. sucrofermentans.…”

Section: Bc Preparation Process From Solid Inoculum (Bcs)mentioning

confidence: 99%

“…The appearance of a several peaks at 1450 − 1300 cm − 1 was further supported the presence of the CH (stretching and bending vibrations of CH group of glucose units). The bands at 1426, 1376, The combined spectra of BCS (Drosos et al 2021) and BCL along with the respective peak position assignments of them are shown in Fig. 6.…”

Section: Thermal Propertiesmentioning

confidence: 99%

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Comparative Study and Characterization of Water-treated Bacterial Cellulose produced by Solid or Liquid inoculum of Komagateibacter sucrofermentans

Drosos

Kordopati

Anastasopoulos

et al. 2023

Preprint

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Structural and physicochemical properties of the water-treated bacterial cellulose (BC) which was produced by Komagateibacter sucrofermentans strain DSM 15973T either from a solid inoculum of the bacterial preculture in HS medium (BCS7) or liquid one (BCL7) after 7 days were investigated in this work. BCL7 was generally proved to be superior to BCS7 regarding BC yield and water holding capacity (WHC). BCL1-7 and BCS7 were analyzed by SEM (Scanning Electron Microscope), FT-IR (Fourier Transform Infrared Spectroscopy), XRD (X-ray diffraction), Nitrogen Adsorption-Desorption Isotherms, TG (thermogravimetric) and DTG (differential thermogravimetric) analysis, in order to investigate the structural modifications and physicomechanical properties of the water-treated BC pellicles. Both BC7 exhibited a high moisture content (98.8%) and the water release content for BCL7 of 1000 mL retained ~12 g of water after 100 h. BCL7 exhibited higher wet mass, specific surface area, pore volume and pore size distribution compared to BCS7. BCL showed an IV(a) isotherm and the crystallinity index of both BC7 were similar (about 55%). BC thermograms followed the same trend and BCS7 reveals a 35% residue up to 780oC. Overall, the resulted structural and physicomechanical differences between BCL and BCS pellicles appears that each could be used for different application.

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Section: Thermal Propertiesmentioning

confidence: 71%

Section: Methodsmentioning

confidence: 99%

Section: Bc Preparation Process From Solid Inoculum (Bcs)mentioning

confidence: 99%

Section: Thermal Propertiesmentioning

confidence: 99%

See 2 more Smart Citations

Comparative Study and Characterization of Water-treated Bacterial Cellulose produced by Solid or Liquid inoculum of Komagateibacter sucrofermentans

Drosos

Kordopati

Anastasopoulos

et al. 2023

Preprint

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“…If a reaction can be completed using intercellular or extracellular enzymes synthesised by specific microbes, the process of biotransformation would be economical for the large-scale synthesis of specialty molecules of industrial importance, such as flavours and aromas for the food industry and optically pure compounds for the pharmaceutical industry [4,5]. Researchers have designed effective cell-factories, incorporating enzymes as well as actively growing whole microbial cells, together in one biocatalyst entity [6][7][8]. Such cell-factories have been employed to perform multi-step (usually 2 to 3) processes for the synthesis of products by biotransformation and at the same time utilizing industrial by-products, towards a contribution to bioeconomy [9,10].…”

Section: Hydrolase Enzymesmentioning

confidence: 99%

Sustainable Biosynthesis of Esterase Enzymes of Desired Characteristics of Catalysis for Pharmaceutical and Food Industry Employing Specific Strains of Microorganisms

Dahiya

Nigam

2022

Sustainability

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Reactions catalysed by sustainably produced enzymes can contribute to the bioeconomy supporting several industries. Low-value compounds can be transformed into added-value products or high-resolution chemicals could be prepared in reactions catalysed by biocatalyst esterase enzymes. These enzymes can be synthesised by purposely isolated or genetically modified strains of microorganisms. Enzymes belonging to the hydrolase family catalyse the formation and hydrolysis of ester bonds to produce the desired esterified molecule. The synthesis of homo-chiral compounds can be accomplished either by chemical or biocatalytic processes, the latter being preferred with the use of microbial esterases. For varied applications, esterases with high stability and retained activity at lower and higher temperatures have been produced with strains isolated from extreme environments. For sustainable production of enzymes, higher productivity has been achieved by employing fast-growing Escherichia coli after incorporating plasmids of required characteristics from specific isolates. This is a review of the isolated and engineered strains used in the biosynthesis of esterase of the desired property, with the objective of a sustainable supply of enzymes, to produce products of industrial importance contributing to the economy.

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Bacterial Nanocellulose‐Based Composite Biocatalysts for Starch‐to‐Bioethanol Valorization under Simultaneous Saccharification and Fermentation

Drosos,

Dima,

Kandylis

et al. 2023

Starch Stärke

Self Cite

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Composite biocatalysts (CB) consisting of amylases and Saccharomyces cerevisiae immobilized separately on bacterial nanocellulose (BNC) are used for the process of simultaneous saccharification and fermentation (SSF) of starch (5%, w/v) for bioethanol production. Parameters such as: i) addition of phosphates and/or divalent metal‐ions salts during the co‐immobilization process of the amylases, ii) required co‐immobilization time, iii) fermentation temperature and initial pH of starch, and iv) CB as single or double freeze‐dried are studied. The utilization of double freeze‐dried CB exhibits fermentation efficiency 89.9% and ethanol yield 0.51 g ethanol g−1 starch while the single freeze‐dried CB 81.1% and 0.46 g ethanol g−1 starch, respectively. In the case of double freeze‐dried CB, the fermentation efficiency decreases by only 27.1% in two‐recycling batches, while in the single freeze‐dried one decreases by 51.3%. The application of double freeze‐dried CB can be used for: i) the eco‐friendly biosynthesis of value‐added bioproducts; ii) the promising option for fuel‐grade bioethanol through starchy wastes or foodstuff starchy residues treatment, and iii) the implementation of industrialization. Finally, to simulate an industrial process of one‐step SSF of starch by applying a CB model, a technoeconomic analysis is evaluated, where using BNCs makes the bioprocess cost‐effective and environmentally favorable, simultaneously.

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A cell-factory model of Saccharomyces cerevisiae based on bacterial cellulose without GMO for consolidated bioprocessing of starch

Cited by 15 publications

References 36 publications

Comparative Study and Characterization of Water-treated Bacterial Cellulose produced by Solid or Liquid inoculum of Komagateibacter sucrofermentans

Comparative Study and Characterization of Water-treated Bacterial Cellulose produced by Solid or Liquid inoculum of Komagateibacter sucrofermentans

Sustainable Biosynthesis of Esterase Enzymes of Desired Characteristics of Catalysis for Pharmaceutical and Food Industry Employing Specific Strains of Microorganisms

Bacterial Nanocellulose‐Based Composite Biocatalysts for Starch‐to‐Bioethanol Valorization under Simultaneous Saccharification and Fermentation

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