A CAPS marker to assist selection of tomato spotted wilt virus (TSWV) resistance in pepper

Moury, B.; Pflieger, Stéphanie; Blattes, A.; Lefebvre, Véronique; Palloix, Alain

doi:10.1139/gen-43-1-137

Cited by 19 publications

(13 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This explains the different degree of success described in the literature. Thus, while several markers were found around the Tsw locus (resistance to tomato spotted wilt virus) using an interspecific cross (Moury et al 2000), we could locate only one marker linked to the Pvr4 locus using 800 primers, and no markers were detected in the vicinity of the Bs3 (resistance to Xanthomonas campestris pv vesicatoria) with 750 primers (Pierre et al 2000), and of Me3 (resistance to Meloidogyne spp.) using 800 primers (DjianCaporalino et al 1998).…”

Section: Discussionmentioning

confidence: 81%

Development of RAPD and SCAR markers linked to the Pvr4 locus for resistance to PVY in pepper (Capsicum annuum L.)

et al. 2002

View full text Add to dashboard Cite

Potato Virus Y (PVY) is the only potyvirus infecting pepper ( Capsicum annuum L.) in Europe. Currently, the development of pepper varieties resistant to PVY seems to be the most-efficient method to control PVY damage. Among the sources of resistance, a monogenic dominant gene Pvr4 confers resistance against all known PVY pathotypes. In this work, bulked segregant analysis (BSA) was used to search for randomly amplified polymorphic DNA (RAPD) markers linked to the Pvr4 gene, using segregating progenies obtained by crossing a homozygous resistant ('Serrano Criollo de Morelos-334') with a homozygous susceptible ('Yolo Wonder') cultivar. Eight hundred decamer primers were screened to identify one RAPD marker (UBC19(1432)) linked in repulsion phase to Pvr4. This marker was converted into a dominant sequence characterised amplified region (SCAR) marker (SCUBC19(1423)). This marker was mapped into a dense Capsicum genetic map in a region where several genes for resistance to different diseases are located. This marker can be useful to identify PVY-resistant genotypes in segregating progenies of pepper in marker-assisted selection (MAS) breeding programs.

show abstract

Section: Discussionmentioning

confidence: 81%

Development of RAPD and SCAR markers linked to the Pvr4 locus for resistance to PVY in pepper (Capsicum annuum L.)

et al. 2002

View full text Add to dashboard Cite

show abstract

“…Two of the above QTLs were mapped in pepper genomic regions where traits of interest were already located. The Lt10.1 QTL mapped in the dominant gene cluster Pvr4 -Tsw controlling respectively the resistance to potyviruses (PepMoV and PVY) and TSWV of pepper (Moury et al 2000;Grube et al 2000). The Lt-6.1 QTL was located near the GC002 marker, corresponding to the gene coding the capsanthin-capsorubin synthase and responsible for the y locus controlling the red-vs. yellow-fruited pepper (Lefebvre et al 1998).…”

Section: Discussionmentioning

confidence: 99%

QTLs for resistance to powdery mildew in pepper under natural and artificial infections

et al. 2003

Self Cite

View full text Add to dashboard Cite

Epidemics of powdery mildew due to Leveillula taurica is an increasing problem in pepper production areas, particularly in coastal regions or greenhouse cultivation. The highly resistant genitor 'H3' was submitted to genetic analysis and QTL mapping in order to promote the introgression of its oligogenic resistance into large and sweet-fruited cultivars. The doubled-haploid progeny from the cross 'H3' (resistant) by 'Vania' (susceptible) was tested for resistance under both natural field infection and artificial inoculation tests, and QTL detection was compared for those two methods. Seven genomic regions including additive QTLs and epistatic interactions were detected, explaining altogether the major part of genotypic variance. Two genomic regions were common to both the evaluation methods, whereas other QTLs were method-specific, reflecting the environment dependence of powdery mildew epidemics. Orthologies with tomato genomic regions carrying resistance genes to L. taurica and Oidium lycopersicum were revealed by comparative mapping with pepper. Tight linkages between the detected QTLs and virus resistance or fruit color traits in pepper were also shown, which adds to the agronomic importance of these regions in pepper breeding programs.

show abstract

“…Two potyvirus R genes, Pvr4, harboured by CM334 , and Pvr7, originating from a Capsicum chinense accession (Grube et al 2000a), were mapped in this region. Tsw conferring resistance to TSWV (Moury et al 2000) and originating from C. chinense was also located in this region (Lefebvre et al 2002). Moreover, this genomic region displayed a strong digenic interaction with the major resistance factor on P5.…”

Section: Highly Conserved Resistance Factors Across Solanaceaementioning

confidence: 92%

Comparative mapping of Phytophthora resistance loci in pepper germplasm: evidence for conserved resistance loci across Solanaceae and for a large genetic diversity

et al. 2003

Self Cite

View full text Add to dashboard Cite

Phytophthora capsici Leonian, known as the causal agent of the stem, collar and root rot, is one of the most serious problems limiting the pepper crop in many areas in the world. Genetic resistance to the parasite displays complex inheritance. Quantitative trait locus (QTL) analysis was performed in three intraspecific pepper populations, each involving an unrelated resistant accession. Resistance was evaluated by artificial inoculations of roots and stems, allowing the measurement of four components involved in different steps of the plant-pathogen interaction. The three genetic maps were aligned using common markers, which enabled the detection of QTLs involved in each resistance component and the comparison of resistance factors existing among the three resistant accessions. The major resistance factor was found to be common to the three populations. Another resistance factor was found conserved between two populations, the others being specific to a single cross. This comparison across intraspecific germplasm revealed a large variability for quantitative resistance loci to P. capsici. It also provided insights both into the allelic relationships between QTLs across pepper germplasm and for the comparative mapping of resistance factors across the Solanaceae.

show abstract

A CAPS marker to assist selection of tomato spotted wilt virus (TSWV) resistance in pepper

Cited by 19 publications

References 7 publications

Development of RAPD and SCAR markers linked to the Pvr4 locus for resistance to PVY in pepper (Capsicum annuum L.)

Development of RAPD and SCAR markers linked to the Pvr4 locus for resistance to PVY in pepper (Capsicum annuum L.)

QTLs for resistance to powdery mildew in pepper under natural and artificial infections

Comparative mapping of Phytophthora resistance loci in pepper germplasm: evidence for conserved resistance loci across Solanaceae and for a large genetic diversity

Contact Info

Product

Resources

About