Comparative mapping of Phytophthora resistance loci in pepper germplasm: evidence for conserved resistance loci across Solanaceae and for a large genetic diversity

Abstract

Phytophthora capsici Leonian, known as the causal agent of the stem, collar and root rot, is one of the most serious problems limiting the pepper crop in many areas in the world. Genetic resistance to the parasite displays complex inheritance. Quantitative trait locus (QTL) analysis was performed in three intraspecific pepper populations, each involving an unrelated resistant accession. Resistance was evaluated by artificial inoculations of roots and stems, allowing the measurement of four components involved in different steps of the plant-pathogen interaction. The three genetic maps were aligned using common markers, which enabled the detection of QTLs involved in each resistance component and the comparison of resistance factors existing among the three resistant accessions. The major resistance factor was found to be common to the three populations. Another resistance factor was found conserved between two populations, the others being specific to a single cross. This comparison across intraspecific germplasm revealed a large variability for quantitative resistance loci to P. capsici. It also provided insights both into the allelic relationships between QTLs across pepper germplasm and for the comparative mapping of resistance factors across the Solanaceae.

Identificación de fuentes de resistencia a pudriciones de la raíz en germoplasma de chile serrano (Capsicum annuum L.)

“…Despite these genetic material did not amplify the SCAR marker OpD04.717, can be used to search for other markers associated with genes that confer resistance to the Oomycete like: CaRGA2, CABPR1, CABGLU, CAPO1, CASC1 (Silvar et al, 2008;Zhang et al, 2013), or QTLs Phyto.4.1, Phyto.5.1, Phyto.6.1, Phyto.11.1 and Phyto12.1, located on chromosomes 4, 5, 11 and 12 respectively (Thabuis et al 2003). Because the resistance to this pathogen is polygenic (Pochard and Daubèze, 1980;Ogundiwin et al, 2005;Minamiyama et al, 2007), it is difficult to explain the resistance to the disease just through marker OpD04.717.…”

“…A pesar que estos materiales genéticos no amplificaron el marcador SCAR OpD04.717, pueden ser utilizados para la búsqueda de otros marcadores asociados a genes que confieren resistencia al Oomycete como: CaRGA2, CABPR1, CABGLU, CAPO1, CASC1 (Silvar et al, 2008;Zhang et al, 2013), o a los QTLs Phyto.4.1, Phyto.5.1, Phyto.6.1, Phyto.11.1 y Phyto12.1, localizadas en los cromosomas 4, 5, 11 y 12, respectivamente (Thabuis et al, 2003). Debido a que la resistencia a este fitopatógeno es poligénica (Pochard y Daubèze, 1980;Minamiyama et al, 2007), es difícil explicar la resistencia a la enfermedad mediante únicamente el marcador OpD04.717.…”

Plant translational genomics: from model species to crops

“…Krutovsky et al 2004). For instance, the tomato and potato genomes, both belonging to the Solanaceae plant family, are remarkably colinear and differ only by five paracentric inversions allowing the identification of CGs across species (Thabuis et al 2003;Huan SanWen et al 2005). On the other hand, caution should be taken with this approach since genomic synteny is not always reflecting a perfect colinearity.…”

Resistance of Chili Pepper Cultivars to Isolates of Phytophthora capsici

“…고추 역병에 대한 유전자원들의 저항성에 관한 연구가 모두 이해된 것은 아니다. 처음에는 독립적으 로 작용하는 2개의 우성유전자에 의해 (Polach and Webster, 1972;Smith et al, 1967) 혹은 modifier와 함께 단일 우성 유전자 (Barksdale et al, 1984)가 저항성을 나타낸다고 하였 으나, 최근에는 고추의 역병 저항성을 질적 저항성보다는 chromosome 4, 5, 6, 11 및 12에 존재하는 6개 영역이 저항 성을 책임지는 QTL 저항성으로 인식하고 있다 (Lefebvre and Palloix, 1996;Lefebvre et al, 2002;Pflieger et al, 2001;Thabuis et al, 2003Thabuis et al, , 2004. 또한 고추의 역병 저항성은 접 종원 농도와 배양시간에 따라 영향을 받으며, 또 고추의 생 육시기가 증가할수록 저항성 발현이 뚜렷하게 증가한다고 알려져 있어 이를 뒷받침한다 (Barksdale et al, 1984;Hwang, 2002;Kim et al, 1989).…”

“…Color demonstrates resistance response of cultivars to each isolate of P. capsici. (Glosier et al, 2008;Lee and Kim, 2012;Oelke et al, 2003;Tamietti and Valentino; (Thabuis et al, 2003(Thabuis et al, , 2004.…”