A bubble column continuous fermentation system for trimethylamine conversion by Aminobacter aminovorans

Lobo, Noel; Rebelo, António; Partidário, Paulo Jorge; Roseiro, J. Carlos

doi:10.1016/s0141-0229(96)00265-7

Cited by 7 publications

(1 citation statement)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microbial degradation of trimethylamine by aerobic bacteria (Colby and Zatman 1973;Boulton and Large 1977;Steenkamp and Mallinson 1976;Large and Haywood 1981;Levering et al 1981;Urakami et al 1990;Ohara et al 1990) and in laboratory-scale bioreactors (Partidário and Carrondo 1993;Lobo et al 1997) have been intensively studied. Although aerobic degradation is effective for the microbial removal of trimethylamine, molecular oxygen may not always be available in contaminated sites, possibly because of its prompt depletion by aerobic metabolism itself and its low solubility in water.…”

Section: Introductionmentioning

confidence: 99%

A novel denitrifying bacterial isolate that degrades trimethylamine both aerobically and anaerobically via two different pathways

Kim

Bae

Lee

2001

Archives of Microbiology

View full text Add to dashboard Cite

The aerobic and anaerobic degradation of trimethylamine by a newly isolated denitrifying bacterium from an enrichment culture with trimethylamine inoculated with activated sludge was studied. Based on 16S rDNA analysis, this strain was identified as a Paracoccus sp. The isolate, strain T231, aerobically degraded trimethylamine, dimethylamine and methylamine and released a stoichiometric amount of ammonium ion into the culture fluid as a metabolic product, indicating that these methylated amines were completely degraded to formaldehyde and ammonia. The strain degraded trimethylamine also under denitrifying conditions and consumed a stoichiometric amount of nitrate, demonstrating that complete degradation of trimethylamine was coupled with nitrate reduction. Cell-free extract prepared from cells grown aerobically on trimethylamine exhibited activities of trimethylamine mono-oxygenase, trimethylamine N-oxide demethylase, dimethylamine mono-oxygenase, and methylamine mono-oxygenase. Cell-free extract from cells grown anaerobically on trimethylamine and nitrate exhibited activities of trimethylamine dehydrogenase and dimethylamine dehydrogenase. These results indicate that strain T231 had two different pathways for aerobic and anaerobic degradation of trimethylamine. This is a new feature for trimethylamine metabolism in denitrifying bacteria.

show abstract