2009
DOI: 10.1016/j.ab.2008.10.001
|View full text |Cite
|
Sign up to set email alerts
|

A fluorescence assay for tetradecyltrimethylammonium mono-oxygenase activity that catalyzes the cleavage of the C–N bond with the production of trimethylamine

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

1
9
0

Year Published

2010
2010
2018
2018

Publication Types

Select...
6
1

Relationship

0
7

Authors

Journals

citations
Cited by 8 publications
(13 citation statements)
references
References 23 publications
1
9
0
Order By: Relevance
“…In the presence of beads with the immobilized bacterial consortium after a 24 h assay, 10 mg L -1 of TTAB were detected in the liquid phase. Taking into account that in P. putida A (ATCC 12633) the TTAB degradation is initiated by Ndealkylation catalyzed by a TTAB monooxygenase activity (Liffourrena et al 2008(Liffourrena et al , 2009, we corroborate that TTAB is metabolized by the immobilized bacterial consortium determining the specific activity of this enzyme. In cell-free extracts obtained from beads removed of the wastewater samples after1 h of assay, the specific activity of TTAB-monooxygenase was 1.22 ±0.10 (n = 3) nmol TMA min -1…”
Section: Resultssupporting
confidence: 67%
See 1 more Smart Citation
“…In the presence of beads with the immobilized bacterial consortium after a 24 h assay, 10 mg L -1 of TTAB were detected in the liquid phase. Taking into account that in P. putida A (ATCC 12633) the TTAB degradation is initiated by Ndealkylation catalyzed by a TTAB monooxygenase activity (Liffourrena et al 2008(Liffourrena et al , 2009, we corroborate that TTAB is metabolized by the immobilized bacterial consortium determining the specific activity of this enzyme. In cell-free extracts obtained from beads removed of the wastewater samples after1 h of assay, the specific activity of TTAB-monooxygenase was 1.22 ±0.10 (n = 3) nmol TMA min -1…”
Section: Resultssupporting
confidence: 67%
“…The cells were disrupted by sonication (20,000 Hz, 10 times for 10 s each time). The whole cells and cell debris were removed by centrifugation (14,000xg for 15 min) and the supernatant was used for TTAB monooxygenase determination by fluorescence, as described (Liffourrena et al, 2009). Protein concentrations were measured by the Bradford method (1976) with bovine serum albumin (Sigma Chemical Co., SL, USA) as a standard.…”
Section: Adsorption Assays In Presence Of Bacterial Immobilized Cellsmentioning
confidence: 99%
“…However, trimethylamine was not degraded by this strain. Cleavage of the C alkyl –N bond as the first degradation step of monoalkonium QACs by a mixed culture, as well as various members of the genus Pseudomonas, was demonstrated in several studies. ,,, In contrast to the previous findings, a recent study proposed the degradation of C 12 TMA-Br, a monoalkonium QAC, by Pseudomonas sp. strain 7-6 isolated from a wastewater treatment plant, via dual pathways.…”
Section: Resultsmentioning
confidence: 96%
“…There was no measurable decline in the concentration of C 14 BDMA, BDMA, and C 14 TMA in the respective cell-free abiotic controls. Liffourrena et al , reported TMA formation from C 14 TMA-Br by Pseudomonas putida ATCC 12633; TMA accumulation ceased the biotransformation of C 14 TMA-Br. Similarly, Patrauchan and Oriel reported the biotransformation of both C 12 BDMA-Cl and C 14 BDMA-Cl by Aeromonas hydrophila K; about 70% of both compounds were utilized, resulting in the production of BDMA and other benzyl-containing amines that inhibited the complete transformation of these two BACs.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation