A fluorescence assay for tetradecyltrimethylammonium mono-oxygenase activity that catalyzes the cleavage of the C–N bond with the production of trimethylamine

Liffourrena, Andrés S.; Boeris, Paola Sabrina; Salvano, Mario A.; Lucchesi, Gloria I.

doi:10.1016/j.ab.2008.10.001

Cited by 8 publications

(13 citation statements)

References 23 publications

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“…In the presence of beads with the immobilized bacterial consortium after a 24 h assay, 10 mg L -1 of TTAB were detected in the liquid phase. Taking into account that in P. putida A (ATCC 12633) the TTAB degradation is initiated by Ndealkylation catalyzed by a TTAB monooxygenase activity (Liffourrena et al 2008(Liffourrena et al , 2009, we corroborate that TTAB is metabolized by the immobilized bacterial consortium determining the specific activity of this enzyme. In cell-free extracts obtained from beads removed of the wastewater samples after1 h of assay, the specific activity of TTAB-monooxygenase was 1.22 ±0.10 (n = 3) nmol TMA min -1…”

Section: Resultssupporting

confidence: 67%

“…The cells were disrupted by sonication (20,000 Hz, 10 times for 10 s each time). The whole cells and cell debris were removed by centrifugation (14,000xg for 15 min) and the supernatant was used for TTAB monooxygenase determination by fluorescence, as described (Liffourrena et al, 2009). Protein concentrations were measured by the Bradford method (1976) with bovine serum albumin (Sigma Chemical Co., SL, USA) as a standard.…”

Section: Adsorption Assays In Presence Of Bacterial Immobilized Cellsmentioning

confidence: 99%

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Degradation of cationic surfactants using immobilized bacteria: Its effect on adsorption to activated sludge

Bergero

Lucchesi

2018

Journal of Biotechnology

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Adsorption of cationic surfactants (QACs) Br-tetradecyltrimethylammonium (TTAB), Cl-tetradecylbenzyldimethylammonium (CBDMA) and Cl-hexadecylbenzyldimethylammonium (CBDMA) to activated sludge from a wastewater treatment plant was tested. Adsorption equilibrium was reached after 2 h, and for initial 200 mg L 81%, 90% and 98% of TTAB, CBDMA and CBDMA were respectively adsorbed. After six successive desorption cycles, 21% of TTAB and 12.7% of CBDMA were desorbed from the sludge. In agreement with the percentage of QACs pre-adsorbed, the more hydrophobic the compound, the lesser the extent of desorption. Wastewater samples with activated sludge were supplemented with TTAB 200 mg L and Ca-alginate beads containing the QACs-degrading microorganisms Pseudomonas putida A (ATCC 12633) and Aeromonas hydrophila MFB03. After 24 h, 10 mg L of TTAB were detected in the liquid phase and 6-8 mg L adsorbed to the sludge. Since without Ca-alginate beads or with empty beads total TTAB amount (phase solid and liquid) did not change, the 90% reduction of the initial 200 mg L after treatment with immobilized cells was attributed to the bacterial consortium's capacity to biodegrade QACs. The results show the advantages of using immobilized bacteria to achieve complete QACs elimination from wastewater systems, thus preventing them from reaching the environment.

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Section: Resultssupporting

confidence: 67%

Section: Adsorption Assays In Presence Of Bacterial Immobilized Cellsmentioning

confidence: 99%

Degradation of cationic surfactants using immobilized bacteria: Its effect on adsorption to activated sludge

Bergero

Lucchesi

2018

Journal of Biotechnology

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“…However, trimethylamine was not degraded by this strain. Cleavage of the C alkyl –N bond as the first degradation step of monoalkonium QACs by a mixed culture, as well as various members of the genus Pseudomonas, was demonstrated in several studies. ,,, In contrast to the previous findings, a recent study proposed the degradation of C 12 TMA-Br, a monoalkonium QAC, by Pseudomonas sp. strain 7-6 isolated from a wastewater treatment plant, via dual pathways.…”

Section: Resultsmentioning

confidence: 96%

“…There was no measurable decline in the concentration of C 14 BDMA, BDMA, and C 14 TMA in the respective cell-free abiotic controls. Liffourrena et al , reported TMA formation from C 14 TMA-Br by Pseudomonas putida ATCC 12633; TMA accumulation ceased the biotransformation of C 14 TMA-Br. Similarly, Patrauchan and Oriel reported the biotransformation of both C 12 BDMA-Cl and C 14 BDMA-Cl by Aeromonas hydrophila K; about 70% of both compounds were utilized, resulting in the production of BDMA and other benzyl-containing amines that inhibited the complete transformation of these two BACs.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, the accumulation of a tertiary amine in the culture medium resulted in the cessation of QAC transformation. 13,31 It was thought that coexistence of at least two species was necessary for the complete mineralization of monoalkonium and dialkonium QACs to ammonia and carbon dioxide under aerobic conditions. 37,38 In the present study, C 14 BDMA-Cl was degraded within 30 h while BDMA was transiently produced at an almost equimolar concentration in the dilute, BAC-degrading community (Figure 3).…”

Section: Environmental Science and Technologymentioning

confidence: 99%

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Aerobic Biotransformation of n-Tetradecylbenzyldimethylammonium Chloride by an Enriched Pseudomonas spp. Community

Tezel

Tandukar

Martinez

et al. 2012

Environ. Sci. Technol.

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The biotransformation of n-tetradecylbenzyldimethylammonium chloride (C 14 BDMA-Cl), a quaternary ammonium compound (QAC), under aerobic conditions by an enriched microbial community growing on benzalkonium chlorides (BACs) was investigated. Biotransformation of C 14 BDMA-Cl commenced with cleavage of the C alkyl −N bond and formation of benzyldimethylamine (BDMA). BDMA was further degraded, but in contrast to a previously reported BAC biotransformation pathway, neither benzylmethylamine (BMA) nor benzylamine (BA) was detected as a BDMA biotransformation product. Kinetic assays further confirmed that BMA and BA were not intermediates of C 14 BDMA-Cl transformation by the enriched community. Thus, BDMA is thought to be transformed to dimethylamine and benzoic acid via debenzylation. The biomass-normalized rate of C 14 BDMA-Cl biotransformation was 0.09 μmol/[mg of volatile suspended solids (VSS)•h]. The Microtox acute toxicity EC 50 value of BDMA was 500 times higher than that of C 14 BDMA-Cl. Thus, the aerobic biotransformation of C 14 BDMA-Cl to BDMA results in substantial toxicity reduction. Phylogenetic analysis of Bacteria diversity indicated that the majority of the sequenced clones (98% of the clone library) belonged to the genus Pseudomonas.

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Role of Quaternary Ammonium Compounds on Antimicrobial Resistance in the Environment

Tezel

Pavlostathis

2011

Antimicrobial Resistance in the Environment

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A fluorescence assay for tetradecyltrimethylammonium mono-oxygenase activity that catalyzes the cleavage of the C–N bond with the production of trimethylamine

Cited by 8 publications

References 23 publications

Degradation of cationic surfactants using immobilized bacteria: Its effect on adsorption to activated sludge

Degradation of cationic surfactants using immobilized bacteria: Its effect on adsorption to activated sludge

Aerobic Biotransformation of n-Tetradecylbenzyldimethylammonium Chloride by an Enriched Pseudomonas spp. Community

Role of Quaternary Ammonium Compounds on Antimicrobial Resistance in the Environment

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