A branched TAT cell-penetrating peptide as a novel delivery carrier for the efficient gene transfection

Jeong, Chan-Uk; Yoo, Jisang; Lee, DaeYong; Kim, Yeu‐Chun

doi:10.1186/s40824-016-0076-0

Cited by 29 publications

(22 citation statements)

References 29 publications

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“…Also, the modified CPPs with cysteine could improve their properties. For example, the branched Tat (BTat), a modified type of Tat (Cys-Tat-Cys-Tat-Cys) with disulfide bonds formed the complexes with plasmid DNA harboring GFP gene and showed higher transfection efficiency (~40-fold) than the Tat/ DNA complexes [218]. Saleh et al demonstrated that covalent linkage of membrane active peptide LK15 to Tat peptide improved its gene transfer likely due to the higher uptake of DNA [219].…”

Section: Nucleic Acid Deliverymentioning

confidence: 99%

Cell penetrating peptides: the potent multi-cargo intracellular carriers

Kardani

Milani

Shabani

et al. 2019

Expert Opinion on Drug Delivery

143

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Introduction: Cell penetrating peptides (CPPs) known as protein translocation domains (PTD), membrane translocating sequences (MTS), or Trojan peptides (TP) are able to cross biological membranes without clear toxicity using different mechanisms, and facilitate the intracellular delivery of a variety of bioactive cargos. CPPs could overcome some limitations of drug delivery and combat resistant strains against a broad range of diseases. Despite delivery of different therapeutic molecules by CPPs, they lack cell specificity and have a short duration of action. These limitations led to design of combined cargo delivery systems and subsequently improvement of their clinical applications. Areas covered: This review covers all our studies and other researchers in different aspects of CPPs such as classification, uptake mechanisms, and biomedical applications. Expert opinion: Due to low cytotoxicity of CPPs as compared to other carriers and final degradation to amino acids, they are suitable for preclinical and clinical studies. Generally, the efficiency of CPPs was suitable to penetrate the cell membrane and deliver different cargos to specific intracellular sites. However, no CPP-based therapeutic approach has approved by FDA, yet; because there are some disadvantages for CPPs including short half-life in blood, and nonspecific CPP-mediated delivery to normal tissue. Thus, some methods were used to develop the functions of CPPs in vitro and in vivo including the augmentation of cell specificity by activatable CPPs, specific transport into cell organelles by insertion of corresponding localization sequences, incorporation of CPPs into multifunctional dendrimeric or liposomal nanocarriers to improve selectivity and efficiency especially in tumor cells.

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Section: Nucleic Acid Deliverymentioning

confidence: 99%

Cell penetrating peptides: the potent multi-cargo intracellular carriers

Kardani

Milani

Shabani

et al. 2019

Expert Opinion on Drug Delivery

143

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“…20 In more recent work, branched polycationic peptides have even been successfully employed as gene transfection tools as well. 21,22…”

Section: Introductionmentioning

confidence: 99%

Efficient cell delivery mediated by lipid‐specific endosomal escape of supercharged branched peptides

Brock

Kustigian

Jiang

et al. 2018

Traffic

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Various densely charged polycationic species, whether of biological or synthetic origin, can penetrate human cells, albeit with variable efficiencies. The molecular underpinnings involved in such transport remain unclear. Herein, we assemble 1, 2 or 3 copies of the HIV peptide TAT on a synthetic scaffold to generate branched cell-permeable prototypes with increasing charge density. We establish that increasing TAT copies dramatically increases the cell penetration efficiency of the peptides while simultaneously enabling the efficient cytosolic delivery of macromolecular cargos. Cellular entry involves the leaky fusion of late endosomal membranes enriched with the anionic lipid BMP. Derivatives with multiple TAT branches induce the leakage of BMP-containing lipid bilayers, liposomal flocculation, fusion and an increase in lamellarity. In contrast, while the monomeric counterpart 1TAT binds to the same extent and causes liposomal flocculation, 1TAT does not cause leakage, induce fusion or a significant increase in lamellarity. Overall, these results indicate that an increase in charge density of these branched structures leads to the emergence of lipid specific membrane-disrupting and cell-penetrating activities.

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“…However, it is rather high molecular size brings toxicity issues for the host cells (Nam et al ., 2015) or decreases the cell metabolic activity (Fischer et al ., 1999; Godbey et al ., 2001; Florea et al ., 2002). Although a CPP alone is unable to properly condense DNA plasmid (Jeong et al ., 2016), the combination of CPPs and PEI significantly improves the transfection efficiency. Moreover, some CPPs namely octaarginine can also inhibit the intracellular proteolytic systems like the proteasome (Kloß et al ., 2009) but it is unknown whether they can permanently inhibit cellular function.…”

Section: Discussionmentioning

confidence: 97%

A simple and efficient transfection protocol for Cryptosporidium parvum using Polyethylenimine (PEI) and Octaarginine

Nguyen-Ho-Bao¹,

Berberich²,

Zheng³

et al. 2020

Parasitology

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The transfection of Cryptosporidium represents a major challenge, and current protocols are based on electroporation of freshly excysted sporozoites using a rather large amount of plasmid DNA which typically has a very poor yield. In this study, we report a fast and simple protocol for transfection of Cryptosporidium parvum that takes advantage of the DNA condensing power of the poly cationic polymer polyethylenimine (PEI) and the gene delivery property of the short cell-penetrating peptide octaarginine. Our novel protocol requires a very low amount of plasmid DNA and does not necessitate special laboratory equipment to be performed. Transfection appears to be more efficient in oocysts just triggered for excystation than the excysted sporozoites. Altogether, the application of octaarginine with PEI allows efficient transfection. To the best of our knowledge, this is the first report on an electroporation-free protocol for transfection of sporozoites of a Cryptosporidium species.

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A branched TAT cell-penetrating peptide as a novel delivery carrier for the efficient gene transfection

Cited by 29 publications

References 29 publications

Cell penetrating peptides: the potent multi-cargo intracellular carriers

Cell penetrating peptides: the potent multi-cargo intracellular carriers

Efficient cell delivery mediated by lipid‐specific endosomal escape of supercharged branched peptides

A simple and efficient transfection protocol for Cryptosporidium parvum using Polyethylenimine (PEI) and Octaarginine

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