A brackishwater isolate of Pseudomonas PS-102, a potential antagonistic bacterium against pathogenic vibrios in penaeid and non-penaeid rearing systems

Abstract

A Pseudomonas sp PS-102 recovered from Muttukkadu brackish water lagoon, situated south of Chennai, showed significant activity against a number of shrimp pathogenic vibrios. Out of the 112 isolates of bacterial pathogens comprising Vibrio harveyi, V. vulnificus, V. parahaemolyticus, V. alginolyticus, V. fluvialis, and Aeromonas spp, 73% were inhibited in vitro by the cell-free culture supernatant of Pseudomonas sp PS-102 isolate. The organism produced yellowish fluorescent pigment on King's B medium, hydrolysed starch and protein, and produced 36.4% siderophore units by CAS assay and 32 μM of catechol siderophores as estimated by Arnow's assay. The PS-102 isolate showed wide ranging environmental tolerance with, temperatures from 25 to 40°C, pH from 6 to 8, salinity from 0 to 36 ppt, while the antagonistic activity peaked in cultures grown at 30°C, pH 8.0 and at 5 ppt saline conditions. The antagonistic activity of the culture supernatant was evident even at 30% v / v dilution against V. harveyi. The preliminary studies on the nature of the antibacterial action indicated that the antagonistic principle as heat stable and resistant to proteolytic, lipolytic and amylolytic enzymes. Pseudomonas sp PS 102 was found to be safe to shrimp when PL-9 stage were challenged at 10 7 CFU ml − 1 and by intramuscular injection into of ∼5 g sub-adults shrimp at 10 5 to 10 8 CFU. Further, its safety in a mammalian system, tested by its pathogenicity to mice, was also determined and its LD 50 to BALB/c mice was found to be 10 9 CFU. The results of this study indicated that the organism Pseudomonas sp PS 102 could be employed as a potential probiont in shrimp and prawn aquaculture systems for management and control of bacterial infections.

Characterization of Bioflocs in a No Water Exchange Super-intensive System for the Production of Food Size Pacific White Shrimp <i>Litopenaeus vannamei</i>

“…Generally, a single or combination of several known cultures isolated from aquacultured shrimp or water used for culture is used to inoculate the system. Organisms from the genus Vibrio (Austin et al, 1995;Balcázar et al, 2007), Bacillus (Gómez and Shen, 2008;Li et al, 2007;Rengipat et al, 2003;Vaseeharan and Ramasay, 2003;Solano and Soto, 2006), Pseudomonas (Vijayan et al, 2006), Roseobacter (Sandaa et al, 2003), and Arthrobacter (Li et al, 2006) have all been employed in shrimp aquaculture or display promise as potential probiotics (reviewed by Ninawe and Selvin, 2009). These bacteria once established within a RAS aid in the maintenance of water quality, most often with respect to nitrification (Kuhn et al, 2010).…”

Selection of Lactobacillus Species from Intestinal Microbiota of Fish for Their Potential Use as Biopreservatives

“…[1,16,19]. Hence the use of such bacteria releasing antimicrobial substances in now gaining importance in fish farming as a natural alternative to administration of antibiotics [1,[61][62][63]. In kinetic studies, both crude bacteriocins were continuously produced during logarithmic phase followed by optimal production during stationary growth phase, suggesting that these peptides may be secondary metabolites.…”

PENGARUH PERBEDAAN WAKTU APLIKASI PROBIOTIK TERHADAP KUALITAS AIR DAN SINTASAN PASCA LARVA UDANG WINDU (Penaeus monodon)

“…Macey & Coyne (2005), melaporkan penggunaan bakteri probiotik pada budidaya abalon. Penggunaan probiotik pada budidaya udang juga telah banyak dilaporkan, seperti pada budidaya udan g w indu (Meunp ol et al, 2 003;Vaseeharan et al, 2004;Lio-Po et al, 2005;Gunarto et al, 2006aGunarto et al, ,2006bVijayan et al, 2006), budidaya udang Penaeus chinensis (Li et al, 2006), budidaya udang vanamei (udang putih) (Nejad et al, 2006), budidaya udang galah (Keysami et al, 2007), pemeliharaan larva udang (Decamp et al, 2008), dan budidaya udang stylorostris (udang putih) (Castex et al, 2008).…”