A biotin—avidin-amplified inhibition enzyme immunoassay for detection of CMV antibodies in human serum

Nielsen, Carsten M.; Hansen, Klaus; Andersen, H. M. K.; Gerstoft, Jan; Vestergaard, Bent Faber

doi:10.1016/0166-0934(87)90004-8

Cited by 10 publications

(7 citation statements)

References 18 publications

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“…For detection of CMV total antibodies an in‐house EIA (enzyme immunoassay) was used. The principle of the test, a biotin‐avidin‐amplified inhibition EIA, was described earlier (14). The results were classified as positive or negative in relation to a cut‐off determined by an inhibition coefficient on 33% calculated by the use of a negative control serum.…”

Section: Methodsmentioning

confidence: 99%

Seropositivity of cytomegalovirus, parvovirus and rubella in pregnant women and recurrent aborters in Leningrad County, Russia

Odland

Sergejeva

Ivaneev

et al. 2001

Acta Obstet Gynecol Scand

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The mean age of the two groups was 27.1 and 28.2 years, respectively. The mean number of deliveries was low (0.4 and 0.5, respectively). Thirty-one point six percent of Group 1 and 41.9% of Group 2 were daily smokers. The group of normally pregnant women had a significantly increased frequency of induced abortions compared to the recurrent aborters, while the recurrent aborters reported more genital infections. There was little difference in total antibodies to cytomegalovirus (CMV) (78.0% and 81.1%, respectively) or B19 IgG (75.3% and 66.9%, respectively) between the groups, while the normal pregnant women had a significantly higher prevalence of rubella antibodies (77.5% and 59.8%, respectively). Our results indicate that less women remain susceptible to primary CMV infection in pregnancy in Russia compared to western Europe and North America. The prevalence of B19 IgG was slightly lower than data from Sweden. Natural immunization against rubella virus was lower than in other, unvaccinated female populations. Vaccination strategies for rubella are now initiated in the Russian Federation. Results from this study are utilized in a federally supported, comprehensive pregnancy care project of North-West Russia.

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Section: Methodsmentioning

confidence: 99%

Seropositivity of cytomegalovirus, parvovirus and rubella in pregnant women and recurrent aborters in Leningrad County, Russia

Odland

Sergejeva

Ivaneev

et al. 2001

Acta Obstet Gynecol Scand

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“…One of the drawbacks on the indirect ELISA is that antibodies to cellular contaminants in the purified HIV antigen preparation can cause false-positive reactions (5,14). We have previously shown that serum antibodies to nonviral epitopes present in the antigen preparation used in an inhibition assay do not cause inhibition (9).…”

Section: Discussionmentioning

confidence: 99%

“…Purification was done by ion-exchange chromatography on DEAE-Tris acryl M (LKB Instruments, Inc., Rock-ville, Md.) in 25 mM Tris, pH 8.8-35 mM NaCl; affinity chromatography was then performed on protein A-Sepharose CL 4B (Pharmacia-LKB, Uppsala, Sweden) as previously described (9). Purified IgG was concentrated by negative-pressure ultrafiltration followed by dialysis against phosphate-buffered saline, pH 7.4, and adjusted to a protein concentration of 5 mg per ml.…”

Section: Methodsmentioning

confidence: 99%

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Antigen-antibody reaction in solution in capture competition immunoassay for human immunodeficiency virus antibodies

1989

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In the capture competition immunoassay, undiluted serum was reacted in solution with purified human immunodeficiency virus (HIV) antigen in wells of microtest plates coated with anti-HIV immunoglobulin G antibodies (HIV capture antibodies). HIV antibodies present in the serum being tested combined with the HIV antigen and thus blocked (completely or partially) the fixation of the antigen to the capture layer. Unblocked antigenic activity was measured in subsequent steps by the use of biotinylated anti-HIV immunoglobulin G and peroxidase-conjugated avidin. The assay was evaluated in comparison with indirect enzyme-linked immunosorbent assay and Western (immuno-) blot (WB). A total of 180 serum samples which reacted repeatedly as positive in indirect enzyme-linked immunosorbent assay but negative in WB were found to be negative by the capture competition assay. Of 54 serum samples showing dubious reactions (single p24 bands in WB), 53 were clearly separated into positive or negative reactions, whereas 1 serum sample gave a borderline reaction. It was concluded that a characteristic feature of this kind of inhibition assay is a very low frequency of equivocal results.

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“…Briefly, IgG and IgM antibodies to C. pneumoniae and to C. trachomatis were measured using the Chlamydia micro-immunofluorescent antibody (MIF) assay (Focus Technologies) which utilized purified Elementary Bodies (EB) as antigens (substrate). 6 In-house assays were used to measure total 18 and IgM 19 antibodies to CMV. HSV-1 and HSV-2 antibodies were measured using the Focus Technologies HerpeSelect™ 1 ELISA IgG assay.…”

Section: Methodsmentioning

confidence: 99%

Prenatal Chlamydia trachomatis infection increases the risk of preeclampsia

Haggerty

Klebanoff

Panum

et al. 2013

Pregnancy Hypertension: An International Journal of Women's Car

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The relationship between Chlamydia trachomatis (CT) and preeclampsia was examined longitudinally among 205 cases and 423 normotensive controls nested within the Collaborative Perinatal Project. Antibodies were analyzed at a first prenatal visit (mean 14.2 weeks) and at delivery. Prenatal infections were identified as IgG/IgM seroconversion or a four-fold rise in IgG antibody titers. Although serological evidence of incident prenatal CT infection was uncommon (n=9, 1.4%) in this general pregnant population, infected women were more likely to develop preeclampsia, after adjustment for maternal age, body mass index, smoking status, race and time between blood draws (ORadj 7.2, 95% CI 1.3 – 39.7).

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A biotin—avidin-amplified inhibition enzyme immunoassay for detection of CMV antibodies in human serum

Cited by 10 publications

References 18 publications

Seropositivity of cytomegalovirus, parvovirus and rubella in pregnant women and recurrent aborters in Leningrad County, Russia

Seropositivity of cytomegalovirus, parvovirus and rubella in pregnant women and recurrent aborters in Leningrad County, Russia

Antigen-antibody reaction in solution in capture competition immunoassay for human immunodeficiency virus antibodies

Prenatal Chlamydia trachomatis infection increases the risk of preeclampsia

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