A Bioartificial Renal Tubule Device Embedding Human Renal Stem/Progenitor Cells

Sciancalepore, Anna Giovanna; Sallustio, Fabio; Girardo, Salvatore; Passione, Laura Gioia; Camposeo, Andrea; Mele, Elisa; Lorenzo, Mirella Di; Costantino, Vincenzo; Schena, Francesco Paolo; Pisignano, Dario

doi:10.1371/journal.pone.0087496

Cited by 71 publications

(40 citation statements)

References 55 publications

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“…The system included a porous polycarbonate membrane sandwiched between two identical PDMS microchannels. Consistent with prior work, applied fluidic shear stress promoted apical and basolateral localization of the AQP2 transporter and Na/K ATPase pump, respectively [46]. …”

Section: Kidney-on-a-chipsupporting

confidence: 86%

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A Role for 3D Printing in Kidney-on-a-Chip Platforms

2016

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The advancement of “kidney-on-a-chip” platforms – submillimeter-scale fluidic systems designed to recapitulate renal functions in vitro – directly impacts a wide range of biomedical fields, including drug screening, cell and tissue engineering, toxicity testing, and disease modelling. To fabricate kidney-on-a-chip technologies, researchers have primarily adapted traditional micromachining techniques that are rooted in the integrated circuit industry; hence the term, “chip.” A significant challenge, however, is that such methods are inherently monolithic, which limits one’s ability to accurately recreate the geometric and architectural complexity of the kidney in vivo. Better reproduction of the anatomical complexity of the kidney will allow for more instructive modelling of physiological and pathophysiological events. Emerging additive manufacturing or “three-dimensional (3D) printing” techniques could provide a promising alternative to conventional methodologies. In this article, we discuss recent progress in the development of both kidney-on-a-chip platforms and state-of-the-art submillimeter-scale 3D printing methods, with a focus on biophysical and architectural capabilities. Lastly, we examine the potential for 3D printing-based approaches to extend the efficacy of kidney-on-a-chip systems.

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Section: Kidney-on-a-chipsupporting

confidence: 86%

“…1c), Sciancalepore and Pisignano et al . cultured adult renal stem/progenitor cells (ARPCs) to model the proximal tubule [46]. The system included a porous polycarbonate membrane sandwiched between two identical PDMS microchannels.…”

Section: Kidney-on-a-chipmentioning

confidence: 99%

A Role for 3D Printing in Kidney-on-a-Chip Platforms

2016

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“…microfluidic devices such as 'organon-a-chip') and 3D cell culture system, which have all been suggested to provide more physiologically representative in vitro systems for studying renal drug disposition (30,50,51). Other emerging technologies, including stem cell science and 3D bio-printing, offer further potential for the development of the next-generation in vitro models (52). The application of these technologies to address questions on renal drug disposition and the translational value of the data generated remains a challenge for the future.…”

Section: Kidney Slicesmentioning

confidence: 99%

Key to Opening Kidney for In Vitro–In Vivo Extrapolation Entrance in Health and Disease: Part I: In Vitro Systems and Physiological Data

Scotcher

Jones²,

Posada

et al. 2016

AAPS J

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ABSTRACT.The programme for the 2015 AAPS Annual Meeting and Exhibition (Orlando, FL; 25-29 October 2015) included a sunrise session presenting an overview of the state-of-the-art tools for in vitro-in vivo extrapolation (IVIVE) and mechanistic prediction of renal drug disposition. These concepts are based on approaches developed for prediction of hepatic clearance, with consideration of scaling factors physiologically relevant to kidney and the unique and complex structural organisation of this organ. Physiologically relevant kidney models require a number of parameters for mechanistic description of processes, supported by quantitative information on renal physiology (system parameters) and in vitro/in silico drug-related data. This review expands upon the themes raised during the session and highlights the importance of high quality in vitro drug data generated in appropriate experimental setup and robust system-related information for successful IVIVE of renal drug disposition. The different in vitro systems available for studying renal drug metabolism and transport are summarised and recent developments involving state-of-the-art technologies highlighted. Current gaps and uncertainties associated with system parameters related to human kidney for the development of physiologically based pharmacokinetic (PBPK) model and quantitative prediction of renal drug disposition, excretion, and/or metabolism are identified.

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“…Another interesting example was given by Sciancalepore et al [126], who developed a bio-inspired renal microdevice aiming to simulate physiological conditions of a kidney proximal tubule. The authors developed a tubule that was populated by adult renal stem/progenitor cells (ARPCs) and supported by a perfused porous polycarbonate membrane.…”

Section: Functional Organ-like Modelsmentioning

confidence: 99%

High-throughput screening approaches and combinatorial development of biomaterials using microfluidics

2016

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Microfluidics, being a technology characterized by the engineered manipulation of fluids at the submillimeter scale, offers some interesting tools that can advance biomedical research and development. Screening platforms based on microfluidic technologies that allow high-throughput and combinatorial screening may lead to breakthrough discoveries not only in basic research but also relevant to clinical application. This is further strengthened by the fact that reliability of such screens may improve, since microfluidic systems allow close mimicking of physiological conditions. Finally, microfluidic systems are also very promising as micro factories of a new generation of natural or synthetic biomaterials and constructs, with finely controlled properties.

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A Bioartificial Renal Tubule Device Embedding Human Renal Stem/Progenitor Cells

Cited by 71 publications

References 55 publications

A Role for 3D Printing in Kidney-on-a-Chip Platforms

A Role for 3D Printing in Kidney-on-a-Chip Platforms

Key to Opening Kidney for In Vitro–In Vivo Extrapolation Entrance in Health and Disease: Part I: In Vitro Systems and Physiological Data

High-throughput screening approaches and combinatorial development of biomaterials using microfluidics

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