A BACTERIOLOGICAL STUDY OF STRAINS OF CORYNE‐BACTERIUM DIPHTHERIAE ISOLATED IN VICTORIA AND NEW SOUTH WALES

Diphthericin types, bacteriophage types and serotypes ofCorynebacterium diphtheriaestrains isolated in Australia

“…The strains isolated in Victoria or received from New South Wales have been described previously (Gibson et al 1970). South Australian strains were forwarded to the Microbiological Diagnostic Unit (M.D.U.)…”

“…The biochemical, serological, bacteriophage-typing and virulence tests were described previously (Gibson et al 1970), but a few additions and amendments were made. The ability to liquefy gelatin was examined by growing the strain, for 14 days at 370 C., on a Loeffler slope upon which an Oxoid charcoal gelatin disk had been placed.…”

“…Type L3a is provisional because the pattern of activity, shown in Table 1, 1963-4, 1967, 19689 Melbourne 1968, 1969, 19703 Swan Hill 1967, 19681 Lismore 1968L5 91 Melbourne 1962-4, 1967-70 33 Sydney 1963, 1967, 1968, 1971 Swan occurred after 24 hr. incubation but, after 72 hr., the pattern changed to that of type L3 since the growths of the indicator diphtheria strains Ferris Nadjarian, Ferris Wagland and 116888 had completely traversed the original diametric streak.…”

“…While the work was being carried out, independent reports of bacteriocin activity among strains of C. diphtheriae appeared from Russia (Emelyanov, Musonova & Lavnik, 1968) and Romania (Meitert, 1969). It was hoped that diphthericin typing would be useful with those strains which could not be typed by serological means or by their sensitivity to bacteriophages (Gibson, Cooper, Saragea & Maximescu, 1970). L. F. GIBsoN AND G. COLMAN…”

Polyacrylamide Gel Electrophoresis of Corynebacterium diphtheriae: a Possible Epidemiological Aid

“…diphtheriae has been traditionally classified into three groups or types (mitis, gravis, and intermedius) on the basis of colonial form, morphological structure, starch fermentations, hemolytic activity, clinical severity of the infection, and serological behavior (9). More recently, differentiation of C. diphtheriae has been made on the basis of phage type (7).…”