Pseudomonas aeruginosa strains isolated from cystic fibrosis patients agglutinate in antisera against anti‐polyagglutinable antigen (PA). Anti‐PA antibodies were formed in rabbits when immunization was carried out with bacteria possessing core‐bound PA, independently of whether the strains were of S or R phenotype. For bacterial agglutination with anti‐PA antibodies two prerequisites are essential: the bacterial cell must be of R phenotype and must possess the core‐linked PA. In contrast, the PA in the isolated LPS's can be demonstrated in passive haemagglutination for both (S or R) phenotypes, provided the PA is core‐linked. Two PA forms have been recognized, one found only in P. aeruginosa species, both in free and bound form. The other one is shared by all members of Pseudomonas genus but is present only in a free, unbound form.
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