A amperometric immunosensor for sensitive detection of circulating tumor cells using a tyramide signal amplification-based signal enhancement system

Zhou, Xiaoyan; Li, Yujian; Wu, Haiping; Huang, Wei; Ju, Huangxian; Ding, Shijia

doi:10.1016/j.bios.2019.01.023

Cited by 37 publications

(15 citation statements)

References 33 publications

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“…Noble metal NPs have attracted growing attention in exploring nanoelectrocatalysts for cytosensors [178,179]. Zhou et al reported an amperometric immunosensor for CTCs detection with PtNPs as nanoelectrocatalysts through a tyramide-based signal ampliiation (TSA) system [180]. As displayed in Figure 10A, PtNPs were functionalized with nucleolin-targeting aptamer AS1411 (CP) and HRP (PtNPs@HRP@CP).…”

Section: Sandwich-like Detectionmentioning

confidence: 99%

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

2021

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The accurate analysis of circulating tumor cells (CTCs) holds great promise in early diagnosis and prognosis of cancers. However, the extremely low abundance of CTCs in peripheral blood samples limits the practical utility of the traditional methods for CTCs detection. Thus, novel and powerful strategies have been proposed for sensitive detection of CTCs. In particular, nanomaterials with exceptional physical and chemical properties have been used to fabricate cytosensors for amplifying the signal and enhancing the sensitivity. In this review, we summarize the recent development of nanomaterials-based optical and electrochemical analytical techniques for CTCs detection, including fluorescence, colorimetry, surface-enhanced Raman scattering, chemiluminescence, electrochemistry, electrochemiluminescence, photoelectrochemistry and so on.

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Section: Sandwich-like Detectionmentioning

confidence: 99%

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

2021

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“…The sensitivity of the developed sensor was very high, with a good linear relationship in the range of 1.0 × 10 2 –1.0 × 10 6 cells/mL, while the detection limit was 34 cells/mL for HeLa cells. Zhou et al developed a sandwich electrochemical sensor with tyrosine signal amplification to detect HeLa cells [ 41 ]. They immobilized the aptamer AS1411 on a gold electrode to capture HeLa cells and used a platinum–horseradish peroxidase–aptamer AS1411 complex as a probe to form a sandwich structure.…”

Section: Electrochemical Detection Of Markers On Tumor Cellsmentioning

confidence: 99%

Electrochemical Sensors for Detection of Markers on Tumor Cells

Zhou

Zhang

2021

IJMS

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In recent years, the increasing incidence and mortality of cancer have inspired the development of accurate and rapid early diagnosis methods in order to successfully cure cancer; however, conventional methods used for detecting tumor cells, including histopathological and immunological methods, often involve complex operation processes, high analytical costs, and high false positive rates, in addition to requiring experienced personnel. With the rapid emergence of sensing techniques, electrochemical cytosensors have attracted wide attention in the field of tumor cell detection because of their advantages, such as their high sensitivity, simple equipment, and low cost. These cytosensors are not only able to differentiate tumor cells from normal cells, but can also allow targeted protein detection of tumor cells. In this review, the research achievements of various electrochemical cytosensors for tumor cell detection reported in the past five years are reviewed, including the structures, detection ranges, and detection limits of the cytosensors. Certain trends and prospects related to the electrochemical cytosensors are also discussed.

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“…The capture probe is fixed on the electrodes to identify and capture CTC. The signal probe is used for amplifying the signal, improving the sensitivity, and reducing the detection limit [27,94,122]. Signal probes are usually some active nanomaterials or particles with enzymatic activity.…”

Section: Sandwich Assaysmentioning

confidence: 99%

Electrochemical Detection and Point-of-Care Testing for Circulating Tumor Cells: Current Techniques and Future Potentials

Han

Sun

et al. 2020

Sensors

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Circulating tumor cells (CTCs) are tumor cells that escaped from the primary tumor or the metastasis into the blood and they play a major role in the initiation of metastasis and tumor recurrence. Thus, it is widely accepted that CTC is the main target of liquid biopsy. In the past few decades, the separation of CTC based on the electrochemical method has attracted widespread attention due to its convenience, rapidness, low cost, high sensitivity, and no need for complex instruments and equipment. At present, CTC detection is not widely used in the clinic due to various reasons. Point-of-care CTC detection provides us with a possibility, which is sensitive, fast, cheap, and easy to operate. More importantly, the testing instrument is small and portable, and the testing does not require specialized laboratories and specialized clinical examiners. In this review, we summarized the latest developments in the electrochemical-based CTC detection and point-of-care CTC detection, and discussed the challenges and possible trends.

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A amperometric immunosensor for sensitive detection of circulating tumor cells using a tyramide signal amplification-based signal enhancement system

Cited by 37 publications

References 33 publications

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

Electrochemical Sensors for Detection of Markers on Tumor Cells

Electrochemical Detection and Point-of-Care Testing for Circulating Tumor Cells: Current Techniques and Future Potentials

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