Electrochemical Sensors for Detection of Markers on Tumor Cells

Abstract

In recent years, the increasing incidence and mortality of cancer have inspired the development of accurate and rapid early diagnosis methods in order to successfully cure cancer; however, conventional methods used for detecting tumor cells, including histopathological and immunological methods, often involve complex operation processes, high analytical costs, and high false positive rates, in addition to requiring experienced personnel. With the rapid emergence of sensing techniques, electrochemical cytosensors have attracted wide attention in the field of tumor cell detection because of their advantages, such as their high sensitivity, simple equipment, and low cost. These cytosensors are not only able to differentiate tumor cells from normal cells, but can also allow targeted protein detection of tumor cells. In this review, the research achievements of various electrochemical cytosensors for tumor cell detection reported in the past five years are reviewed, including the structures, detection ranges, and detection limits of the cytosensors. Certain trends and prospects related to the electrochemical cytosensors are also discussed.

Advanced electrocatalytic materials based biosensors for cancer cell detection – A review

“…This aptasensor effectively detected a specific breast cancer cell of SKBR-3 (which is responsible for the transfer of cancer cells to the other organs) from 20 to 2000 cells/mL with the LOD of 20 cells/mL. Also, the repeatability of the cytosensor was very good with a Relative Standard Deviation (RSD) of 1.6 % for 500 cells/ F I G U R E 6 (a) Preparation of EC cytosensors [4] (open access). (b) L-type multi-layered cytosensor for the detection of cancer cells [73].…”

“…Advanced cancer cell detection techniques are required to successfully treat cancer. In this aspect, electrochemical cytosensors have garnered considerable interest for tumor cell identification [4,5]. Observing CTCs in blood cells can provide significant information for cancer diagnosis, prediction, and treatment.…”

Nanomaterials for Molecular Detection and Analysis of Extracellular Vesicles

“…An electrochemical biosensor is a device that detects biomarkers by converting molecular interactions into electronic readout signals [ 85 , 86 ]. They have been widely used for EV detection [ 87 , 88 , 89 , 90 , 91 , 92 , 93 ].…”

Stretchable Electrochemical Sensors: From Electrode Fabrication to Cell Mechanotransduction Monitoring†

“…In general, millimeter‐sized electrodes allow living cells to be cultured directly thereon, and this sensing mode combined with biorecognition strategies are widely employed to evaluate the metabolic activity of bulk cells. [ 11‐16 ] Microelectrode or nanoelectrode with high temporal‐spatial resolution can be positioned near or inside individual cells to monitor single cell behaviors ( e.g ., exocytosis) [ 17‐21 ] or subcellular processes ( e.g ., intracellular events). [ 22‐28 ] These achievements greatly promote the understanding of cellular metabolism, cell‐cell communication and cell‐matrix interactions.…”