A 3D graphene oxide microchip and a Au-enwrapped silica nanocomposite-based supersandwich cytosensor toward capture and analysis of circulating tumor cells

Li, Na; Xiao, Tingyu; Zhang, Zhengtao; He, Rongxiang; Wen, Dan; Cao, Yiping; Zhang, Weiying; Chen, Yong

doi:10.1039/c5nr04798f

Cited by 31 publications

(14 citation statements)

References 35 publications

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“…Microfluidic chips appear as an attractive alternative to conventional biochemical methods offering a “liquid biopsy” 11 utilizing immune-affinity, physical separation and immuomagnetic approaches. CTC-chip 12 , Herringbone-chip (HB-Chip) 13 , patterning regions of alternating adhesive proteins 14 , a grapheme oxide chip 15 , a 3D graphene oxide microchip 16 , OncoBean chip 17 and a GO-polymer device 18 realize affinity-based isolation. The abovementioned approaches resort to a specific antigen, EpCAM or aptamers 19,20 .…”

Section: Introductionmentioning

confidence: 99%

Highly-sensitive capture of circulating tumor cells using micro-ellipse filters

Chen

Cao

Sun

et al. 2017

Sci Rep

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Circulating tumor cells (CTCs) detection, enumeration and characterization with microfluidic chips has critical significance in cancer prognosis offering a non-invasive “liquid biopsy”. Based on physical differences of size and deformability, we explore micro-ellipse filters consisting of microfuidic slits in series gradually narrowed. Slender tunnels sensitively capture tumor cells with slim chance to escape. Tumor cells could reside at capture sites organized by arrays of micro-ellipse microposts enduring less stress. Circular elliptical microstructures produce smooth flow minimally reducing any damage. “Air Suction” could extremely shorten capture. Capture efficiency comes out to be a robust yield of 90% and percentage obeys Gaussian distribution at various stages. With rare number accurately enumerated, micro-Ellipse filters have been tested high efficiently capturing tumor cells in both whole and lysed blood. To clinically validate the device, the microfluidic chip was utilized to identify and capture CTCs from metastatic breast, colon and non-small-cell lung (NSCLC) cancer patients. CTCs were detected positive in all samples with 4 patients having more than 20 CTCs. Those sensitive results are consistent with theoretical expectation. Efficient micro-ellipse filters enable clinical enumeration of metastasis, on-chip anti-cancer drug responses and biological molecular analysis.

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Section: Introductionmentioning

confidence: 99%

Highly-sensitive capture of circulating tumor cells using micro-ellipse filters

Chen

Cao

Sun

et al. 2017

Sci Rep

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“…In detail, researchers have already started exploring the use of G at the CNS for cell labeling and real-time live-cell monitoring (Wang et al, 2014 ; Zuccaro et al, 2015 ); delivery to the brain of molecules that are usually rejected by the BBB (Tonelli et al, 2015 ; Dong et al, 2016 ); G-based scaffolds for cell culture (Li N. et al, 2013 ; Menaa et al, 2015 ; Defterali et al, 2016b ); and cell analysis based on G-electrodes (Medina-Sánchez et al, 2012 ; Li et al, 2015 ). In addition, interfacing G with neural cells was also proposed to be extremely advantageous for exploring their electrical behavior or facilitating neuronal regeneration by promoting controlled elongation of neuronal processes (Li et al, 2011 ; Tu et al, 2014 ; Fabbro et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Interfacing Graphene-Based Materials With Neural Cells

Bramini

Alberini

Colombo

et al. 2018

Front. Syst. Neurosci.

100

114

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The scientific community has witnessed an exponential increase in the applications of graphene and graphene-based materials in a wide range of fields, from engineering to electronics to biotechnologies and biomedical applications. For what concerns neuroscience, the interest raised by these materials is two-fold. On one side, nanosheets made of graphene or graphene derivatives (graphene oxide, or its reduced form) can be used as carriers for drug delivery. Here, an important aspect is to evaluate their toxicity, which strongly depends on flake composition, chemical functionalization and dimensions. On the other side, graphene can be exploited as a substrate for tissue engineering. In this case, conductivity is probably the most relevant amongst the various properties of the different graphene materials, as it may allow to instruct and interrogate neural networks, as well as to drive neural growth and differentiation, which holds a great potential in regenerative medicine. In this review, we try to give a comprehensive view of the accomplishments and new challenges of the field, as well as which in our view are the most exciting directions to take in the immediate future. These include the need to engineer multifunctional nanoparticles (NPs) able to cross the blood-brain-barrier to reach neural cells, and to achieve on-demand delivery of specific drugs. We describe the state-of-the-art in the use of graphene materials to engineer three-dimensional scaffolds to drive neuronal growth and regeneration in vivo, and the possibility of using graphene as a component of hybrid composites/multi-layer organic electronics devices. Last but not least, we address the need of an accurate theoretical modeling of the interface between graphene and biological material, by modeling the interaction of graphene with proteins and cell membranes at the nanoscale, and describing the physical mechanism(s) of charge transfer by which the various graphene materials can influence the excitability and physiology of neural cells.

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“…Mounting evidence suggests that circulating tumor cells (CTCs), which can shed from a primary tumor mass at the earliest stages of malignant progression, play a critical role in cancer metastasis[ 1 - 3 ]. As a “liquid biopsy” for tumor, CTCs provide an insight into tumor biology in a critical window where intervention could actually make a difference[ 4 - 7 ].…”

Section: Introductionmentioning

confidence: 99%

Epidermal growth factor receptor-targeted immune magnetic liposomes capture circulating colorectal tumor cells efficiently

Kuai¹,

Wang²,

Zhang³

et al. 2018

WJG

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AIMTo compare the capacity of newly developed epidermal growth factor receptor (EGFR)-targeted immune magnetic liposomes (EILs) vs epithelial cell adhesion molecule (EpCAM) immunomagnetic beads to capture colorectal circulating tumor cells (CTCs).METHODSEILs were prepared using a two-step method, and the magnetic and surface characteristics were confirmed. The efficiency of capturing colorectal CTCs as well as the specificity were compared between EILs and EpCAM magnetic beads.RESULTSThe obtained EILs had a lipid nanoparticle structure similar to cell membrane. Improved binding with cancer cells was seen in EILs compared with the method of coupling nano/microspheres with antibody. The binding increased as the contact time extended. Compared with EpCAM immunomagnetic beads, EILs captured more CTCs in peripheral blood from colorectal cancer patients. The captured cells showed consistency with clinical diagnosis and pathology. Mutation analysis showed same results between captured CTCs and cancer tissues.CONCLUSIONEGFR antibody-coated magnetic liposomes show high efficiency and specificity in capturing colorectal CTCs.

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A 3D graphene oxide microchip and a Au-enwrapped silica nanocomposite-based supersandwich cytosensor toward capture and analysis of circulating tumor cells

Cited by 31 publications

References 35 publications

Highly-sensitive capture of circulating tumor cells using micro-ellipse filters

Highly-sensitive capture of circulating tumor cells using micro-ellipse filters

Interfacing Graphene-Based Materials With Neural Cells

Epidermal growth factor receptor-targeted immune magnetic liposomes capture circulating colorectal tumor cells efficiently

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