Epidermal growth factor receptor-targeted immune magnetic liposomes capture circulating colorectal tumor cells efficiently

Abstract

AIMTo compare the capacity of newly developed epidermal growth factor receptor (EGFR)-targeted immune magnetic liposomes (EILs) vs epithelial cell adhesion molecule (EpCAM) immunomagnetic beads to capture colorectal circulating tumor cells (CTCs).METHODSEILs were prepared using a two-step method, and the magnetic and surface characteristics were confirmed. The efficiency of capturing colorectal CTCs as well as the specificity were compared between EILs and EpCAM magnetic beads.RESULTSThe obtained EILs had a lipid nanoparticle structure similar to cell membrane. Improved binding with cancer cells was seen in EILs compared with the method of coupling nano/microspheres with antibody. The binding increased as the contact time extended. Compared with EpCAM immunomagnetic beads, EILs captured more CTCs in peripheral blood from colorectal cancer patients. The captured cells showed consistency with clinical diagnosis and pathology. Mutation analysis showed same results between captured CTCs and cancer tissues.CONCLUSIONEGFR antibody-coated magnetic liposomes show high efficiency and specificity in capturing colorectal CTCs.

<p>Construction and Characterization of KRAS Immune Lipid Magnetic Balls for Colorectal Cancer Circulating Tumor Cells</p>

“…There have been studies on the preparation of EGFR antibody-modified lipid magnetic balls for CTC detection of CRC and detection of KRAS mutations, with a KRAS mutation rate of 71.4% (5/7). 28 The number of samples included in this study is too small and not representative. We used KRAS antibody-modified lipid magnetic balls to be more targeted.…”

Lung carcinoma therapy using epidermal growth factor receptor‑targeted lipid polymeric nanoparticles co‑loaded with cisplatin and doxorubicin

“…Gill and colleagues reported the preparation of poly(lactic-co-glycolic acid) (PLGA) nanoparticles surface conjugated to diethylenetriaminepentaacetic acid-human EGF encapsulating the ruthenium-based DNA replication inhibitor and radiosensitizer for EGFR-targeted combination therapy in esophageal cancer cells (35). Kuai et al designed EGFR-targeted immune magnetic liposomes which could capture circulating colorectal tumor cells efficiently (36). In the present study, EGF-PEG-DSPE was synthesized and applied as materials for lipid polymeric nanoparticle (LPN) preparation.…”

Recent Advances of Magnetic Nanomaterials in Bone Tissue Repair

“…In addition to treating infection, the Fe 3 O 4 nanoparticles in the composite can also promote the adhesion, proliferation, and osteogenic differentiation of bone marrow mesenchymal stem cells (Xia et al, 2018 ; Swietek et al, 2019 ). Nanomaterials have been used as carriers to deliver therapeutic drugs in cells, including proteins, growth factors, small molecule chemicals, and DNA/RNA (Cruz-Acuña et al, 2018 ; Kuai et al, 2018 ; Xia et al, 2018 , 2019a ). This magnetic drug delivery system will provide a useful support platform for bone defect repair.…”

“…Tissue engineering is a method of rebuilding functional tissue at the damaged site, usually including seed cells, growth factors, and three-dimensional biodegradable scaffolds (Cruz-Acuña et al, 2018 ; Kuai et al, 2018 ; Luo et al, 2018 ; Cojocaru et al, 2019 ; Li et al, 2020 ). The construction of a tissue engineering complex is of great significance to promote tissue regeneration or healing.…”

“…At present, the role of magnetic scaffolds in promoting cell proliferation and new bone tissue growth has been confirmed (Lu et al, 2018 ). The scaffold has a wide range of components, mainly including biological macromolecules, synthetic polymers, polyethylene glycol, and inorganic materials (Dashnyam et al, 2014 ; Cruz-Acuña et al, 2018 ; Kuai et al, 2018 ; Luo et al, 2018 ; Cojocaru et al, 2019 ; Liu Z. Y. et al, 2020 ).…”