The purpose of this study was to prepare and characterize a lipid magnetic ball modified with KRAS antibodies on the surface and to isolate circulating tumor cells of colorectal cancer with KRAS mutations. Methods: The microemulsion method was used to form lipid bilayers to encapsulate Fe3O4 nanoparticles with superparamagnetism to form lipid magnetic balls, and KRAS antibodies were formed on the surface to form KRAS immune lipid magnetic balls. Results: Compared with traditional EpCAM antibody-modified lipid magnetic balls, it can effectively improve the capture ability of colorectal cancer circulating tumor cells with KRAS mutation, the capture rate reaches 92.9%, and the capture results are consistent with clinical diagnosis and pathology. Conclusion: Our results showed that KRAS antibody-modified lipid magnetic balls can be used in the diagnosis and treatment of KRAS colorectal cancer.
Abstract:The free vibration characteristics of functionally graded micro-switches under combined electrostatic, axial residual stress and temperature change is investigated, with an emphasis on the effect of geometric nonlinear deformation due to mid-plane stretching, the influence of volume fraction profile parameter and temperature change. The micro-switch considered in this study is made of either homogeneous material or non-homogeneous functionally graded material with two material phases. Taking the temperature-dependency of the effective material properties into consideration, the Voigt model is used to simulate the material properties of the FGMs (functionally graded materials). The principle of virtual work is used to derive the nonlinear governing differential equation. The eigenvalue problem which describes free vibration of the micro-beam at its statically deflected state is then solved using DQM (differential quadrature method). The natural frequencies of clamped-clamped micro-switches are obtained. The solutions are validated through direct comparisons with experimental results reported in previous studies. A parametric study is conducted to show the effects of geometric nonlinearity, material composition, temperature change and geometrical parameters for the natural frequencies.
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