Highly-sensitive capture of circulating tumor cells using micro-ellipse filters

Chen, Hongmei; Cao, Bangwei; Sun, Bo; Cao, Yapeng; Yang, Ke; Lin, Yu‐Sheng

doi:10.1038/s41598-017-00232-6

Cited by 54 publications

(45 citation statements)

References 37 publications

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“…Many platforms have been developed on the basis of biological properties and physical properties in the detection CTCs for cancer diagnosis . Because sarcoma is derived from mesenchymal origin, detection using the FDA approved approach (EpCAM) may not be optimal.…”

Section: Discussionmentioning

confidence: 99%

Metastatic tumor cells detection and anti‐metastatic potential with vesicular stomatitis virus in immunocompetent murine model of osteosarcoma

Johan

Kubo

Furuta

et al. 2018

Journal Orthopaedic Research

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Sarcomas are associated with a high incidence of lung metastasis, which leads to a high-risk of cancer death. This study was performed to explore the pre-clinical theranostic potential of a novel fully functional recombinant vesicular stomatitis virus carrying imaging gene Katushka (rVSV-K), as virotherapy and circulating tumor cells (CTCs) detection in the syngeneic mouse model of osteosarcoma with spontaneous pulmonary metastases. Recombinant VSV-K was generated and evaluated in vitro on human and murine osteosarcoma cells. Spontaneous osteosarcoma metastases were established in immune-competent mice by implanting subcutaneously syngeneic osteosarcoma LM8 cells. The vector was injected into the tumor-bearing mice via jugular vein either once or repeatedly. To assess effectiveness, primary tumor growth and development of lung metastasis as well as survival were evaluated. We found that rVSV-K efficiently replicated in and killed all osteosarcoma cell lines in time-dependent manners. Both single or repeated systemic injections of the virus did not inhibit the growth of the primary tumor, but the repeated administration could effectively suppress the development of lung metastases and was likely responsible for the observed increase in survival. Furthermore, we demonstrated, for the first time, that CTCs in blood samples from syngeneic osteosarcoma-bearing mice were successfully detected by utilizing rVSV-K ex vivo. Our results show that repeated systemic injections of rVSV-K are an effective anti-metastatic agent against osteosarcoma in immune-competent mice and this virus to be a useful tool for detection of osteosarcoma CTCs, suggesting that further development of future viral-based theranostic approach in patients with osteosarcoma is warranted. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:2562-2569, 2018.

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Section: Discussionmentioning

confidence: 99%

Metastatic tumor cells detection and anti‐metastatic potential with vesicular stomatitis virus in immunocompetent murine model of osteosarcoma

Johan

Kubo

Furuta

et al. 2018

Journal Orthopaedic Research

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“…Sometimes, microfluidic devices show low sensitivity and reproducibility (due to size overlap between CTCs and WBCs). This limitation was recently addressed by a device made of micro-ellipse filters with different arrays of 12 micro-filters that can reliably capture viable CTCs with high purity and efficiency without hydrodynamic forces [129].…”

Section: Inertial Focusingmentioning

confidence: 99%

The Role of Circulating Tumor Cells in the Metastatic Cascade: Biology, Technical Challenges, and Clinical Relevance

Dianat‐Moghadam

Azizi

Eslami-S

et al. 2020

Cancers

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Metastases and cancer recurrence are the main causes of cancer death. Circulating Tumor Cells (CTCs) and disseminated tumor cells are the drivers of cancer cell dissemination. The assessment of CTCs’ clinical role in early metastasis prediction, diagnosis, and treatment requires more information about their biology, their roles in cancer dormancy, and immune evasion as well as in therapy resistance. Indeed, CTC functional and biochemical phenotypes have been only partially characterized using murine metastasis models and liquid biopsy in human patients. CTC detection, characterization, and enumeration represent a promising tool for tailoring the management of each patient with cancer. The comprehensive understanding of CTCs will provide more opportunities to determine their clinical utility. This review provides much-needed insights into this dynamic field of translational cancer research.

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“…The interaction mechanism of CTCs with the antibody (EpCAM)‐coated microposts, separates CTCs from several clinical samples with approximately 50% purity in 99% of clinical samples (Nagrath et al, ; Sequist, Nagrath, Toner, Haber, & Lynch, ). Separation of CTCs from the metastatic colon, breast, and nonsmall‐cell lung with 90% capture efficiency is reported using microfluidic chips with microellipse filters (H. Chen et al, ). High‐throughput viable CTC isolation using low‐cost fabric ﬁlters were successful for the isolation of both mesenchymal and epithelial cancer cells with about 92% efficient retrieval and 86% viable capture (Bu et al, ).…”

Section: Downstream Characterization Of Ctcs Captured On Microfiltersmentioning

confidence: 99%

Filter‐based isolation, enrichment, and characterization of circulating tumor cells

Khetani

Mohammadi

Nezhad

2018

Biotech & Bioengineering

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Isolation of circulating tumor cells (CTCs) from blood has long been a challenge due to the rarity and heterogeneity of these cells. Detection technologies have predominantly focused on different molecular or physical properties of CTCs. Size-based isolation approach using microfilters have been widely used to capture CTCs because of the difference in size and stiffness of the cells compared to other hemocytes. Isolation of rare cells based on their size was the original CTC enrichment technique and it demonstrated a simple yet rapid method that enhanced the recovery of cells with high throughput. In this review, we highlight key technical aspects of filter-based isolation, detection, and characterization of CTCs, and compare the clinical performance of filter-based devices with the approved platforms and immunoassays used for the analysis of CTCs. We have also discussed future prospective and incorporation of advances in immunochemistry technique into the filter-based platforms for enhancing the utility in clinical settings.

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Highly-sensitive capture of circulating tumor cells using micro-ellipse filters

Cited by 54 publications

References 37 publications

Metastatic tumor cells detection and anti‐metastatic potential with vesicular stomatitis virus in immunocompetent murine model of osteosarcoma

Metastatic tumor cells detection and anti‐metastatic potential with vesicular stomatitis virus in immunocompetent murine model of osteosarcoma

The Role of Circulating Tumor Cells in the Metastatic Cascade: Biology, Technical Challenges, and Clinical Relevance

Filter‐based isolation, enrichment, and characterization of circulating tumor cells

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