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Koenigbauer, Michael J.

doi:10.1023/a:1018957103417

Cited by 33 publications

(6 citation statements)

References 21 publications

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“…Isothermal microcalorimetry has found a wide range of applications, i.e., in pharmaceutical industry, life science research, − and chemical industry. , However, as far as we know, isothermal calorimetry has not been used in studying the thermal denaturation of β-lg or other proteins. The aim of this study was to investigate the potential for isothermal microcalorimetry in the study of the thermal denaturation and aggregation of β-lg.…”

Section: Introductionmentioning

confidence: 99%

Isothermal and Scanning Calorimetry Measurements on β-Lactoglobulin

et al. 1997

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The denaturation and aggregation of β-lactoglobulin was studied by isothermal calorimetry. Experiments with several β-lactoglobulin concentrations (15−100 g/L) were performed at temperatures in the range 62−68.5 °C. Even a small change in temperature had a tremendous effect on the shape of the thermograms, depending also very strongly on the β-lactoglobulin concentration used. The measured thermograms were modeled using the kinetic model for the denaturation and aggregation of β-lactoglobulin recently developed by Roefs and De Kruif. In this kinetic model we recognize four consecutive steps: dissociation, unfolding, exchange of disulfide bonds, and aggregation. The numerical calculations yielded results that were in qualitative and quantitative agreement with typical experimental isothermal calorimetry curves. DSC curves and the decrease in concentration of native β-lactoglobulin as a function of time could also be reproduced well. We think that isothermal calorimetry, in combination with this kinetic model, provides a valuable and useful approach to the study of the denaturation and aggregation of β-lactoglobulin and other proteins.

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Section: Introductionmentioning

confidence: 99%

Isothermal and Scanning Calorimetry Measurements on β-Lactoglobulin

et al. 1997

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“…Determination of φ t as a function of time at several temperatures is usually required for obtaining the temperature dependence of the rate law. Isothermal calorimetry has been shown to be a faster and more accurate method for obtaining activation energies than methods involving analysis of the sample as a function of time …”

Section: Temperature Dependence Of the Rate Lawmentioning

confidence: 99%

“…Much effort has been expended to develop methods for the simultaneous determination of the rate law and its temperature dependence by temperature scanning calorimetry. Such an approach has both theoretical and experimental difficulties. − Resolution of many of these difficulties has occurred only recently. − Thus, rate laws derived from temperature scanning methods must be regarded with skepticism, particularly from older literature. Further discussion of this topic is beyond the scope of this review.…”

Section: Temperature Dependence Of the Rate Lawmentioning

confidence: 99%

Calorimetric Measurement of the Kinetics of Slow Reactions

Hansen

2000

Ind. Eng. Chem. Res.

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A brief review of the application of calorimetry to measurement of the kinetics of slow processes is presented. Processes degrading or otherwise changing the properties of materials often occur too slowly to be readily measured by conventional chemical analyses but can be measured in a relatively short period of time by heat conduction calorimetry. Instrument selection and derivation of rate laws from calorimetric data are discussed, and illustrative examples are presented.

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“…Microcalorimetry is useful in the study of physical, chemical, and biological drug interactions, 17 from which a lot of useful information can be obtained. Microcalorimetry is also an important tool to characterize drug formulations and pharmaceutical polymorphs, 18 and to predict the binding affinity of small-molecule ligands to macromolecular receptors.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Thioproline Salicylic Acid Samarium Complex and Microcalorimetric Study on Effects of the Complex on the Growth Metabolism of S. pombe Cells

Li¹,

Zhang²,

Li³

et al. 2011

Chin. J. Chem.

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The product from reaction of samarium chloride hexahydrate with salicylic acid and thioproline, [Sm(C 7 H 5 O 3 ) 2 •(C 4 H 6 NO 2 S)]•2H 2 O (TSAS), was synthesized and characterized by IR, elemental analysis and thermogravimatric analysis. Particularly, the effect of the compounds TSAS, SmCl 3 •6H 2 O, C 7 H 6 O 3 and C 4 H 7 NO 2 S on the growth and metabolism of S. pombe by a TAM Air isothermal calorimeter at 32 ℃ is also reported. The thermokinetic parameters, which included the microbial growth rate constant (κ), inhibition ratio (I) and half inhibition concentration (IC 50 ), were obtained. The results showed that all the compounds TSAS, SmCl 3 •6H 2 O, C 7 H 6 O 3 and C 4 H 7 NO 2 S possessed the bi-directional biological effect and Hormesis effect. These stimulate the growth of the S. pombe at lower concentration, but inhibit the growth at higher concentration. The half inhibition concentrations (IC 50 ) of TSAS, SmCl 3 •6H 2 O, C 4 H 7 NO 2 S and C 7 H 6 O 3 were found to be 0.732, 0.746, 0.746 and 1.43 mmol•L -1 , respectively. The inhibition ability of these compounds on the growth of the S. pombe has been observed to decrease in the order TSAS＞SmCl 3 •6H 2 O＝C 4 H 7 NO 2 S＞C 7 H 6 O 3 . Moreover, the effect of TSAS on the growth and cytokinesis of S. pombe was investigated by cytomorphology and FT IR spectroscopy technique. The results indicated that TSAS had a direct impact on apoptosis, and could also inhibit the cell growth by reducing cytokinesis and the binding locations of drug (TSAS) in S. pombe cells could be the proteins and nucleic acid.

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Cited by 33 publications

References 21 publications

Isothermal and Scanning Calorimetry Measurements on β-Lactoglobulin

Isothermal and Scanning Calorimetry Measurements on β-Lactoglobulin

Calorimetric Measurement of the Kinetics of Slow Reactions

Synthesis of Thioproline Salicylic Acid Samarium Complex and Microcalorimetric Study on Effects of the Complex on the Growth Metabolism of S. pombe Cells

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