Untitled

Abstract

We examined different fluorescent probes suitable for fluorometric determination of alpha 1-acid glycoprotein (AGP) in serum. Quinaldine red (QR) was shown to bind strongly and selectively to AGP. Taking advantage of the enhanced fluorescence of QR in the presence of AGP, we developed a direct method for the determination of serum AGP without removal of other serum proteins such as albumin. AGP concentrations in serum of healthy volunteers and patients correlated well with results from the conventional single radial immunodiffusion (SRID) method (r = 0.93, slope = 1). The newly developed method is faster and has a larger analytical concentration range than the SRID method. This method can also be used to determine AGP in serum of experimental animals, and it can serve to monitor AGP serum concentrations for pharmacokinetic evaluation of basic drugs.

Characterization of binding mode of imatinib to human α1-acid glycoprotein

“…A similar phenomenon (i.e. enhanced fluorescence intensity and decreased concentration of QR bound to AGP) was found in the presence of androstanedione [35]. Ternary complex formation on AGP have been reported between dicumarol and some basic compounds, revealed by inversion o ICD spectra of bound dicumarol [11,13,38].…”

Role of Orosomucoid in the Regulation of Plasma Proteolytic Systems during Experimental Renal Failure

“…units of 50% hemolysis. AGP concentration in the plasma and kidney homogenate was measured on a Fluoroscan-II fl uorometer (Labsystems) with a fl uorescent dye quinaldine red (Sigma-Aldrich) [9]. The results were analyzed by Statistica 6.0 software.…”

High-performance liquid chromatography determination of α1-acid glycoprotein in small volumes of plasma from neonates

“…Methods based on binding the dyes quinaldine red [9] and auramine O [10] have been reported for the determination of AGP. Although these methods are conveniently able to determine AGP without a previous isolation step, they suffer from disadvantages such as long analysis times, lack of sensitivity at low concentrations or the need for large sample volumes.…”