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Kumar, Harsh; Murthy, Hosakatte Niranjana; Paek, Kee-Yoeup

doi:10.1023/a:1016529322046

Cited by 30 publications

(5 citation statements)

References 6 publications

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“…Factors that influence the in vitro morphogenesis includes explant type and its physiological ontogenetic age, the season of collection and size, among others [7, 20]. In the case of G. sylvestre and many species, the in vitro morphogenesis could only be achieved when explants used to initiate the in vitro cultures are at a certain stage of development [6, 17, 19]. In previous studies, the indirect plant regeneration system of G. sylvestre were attempted using mature explant tissues of leaf, hypocotyl, cotyledons and nodal segment explants [17, 21, 22].…”

Section: Discussionmentioning

confidence: 99%

“…In the case of G. sylvestre and many species, the in vitro morphogenesis could only be achieved when explants used to initiate the in vitro cultures are at a certain stage of development [6, 17, 19]. In previous studies, the indirect plant regeneration system of G. sylvestre were attempted using mature explant tissues of leaf, hypocotyl, cotyledons and nodal segment explants [17, 21, 22]. This prompted the experiment reported herein using ex vitro nodal segment of mature plants as an initial explant used to initiate micro shoot and from which the plant regeneration system was developed using micro shoot apical meristem.…”

Section: Discussionmentioning

confidence: 99%

“…Khatak et al [22] obtained green callus cultures from leaf explants of G. sylvestre and pigmentation of the callus increased with increase in the concentration of cytokinins amended in the cultivation medium and subculture cycles, without morphogenesis. However, Ashok Kumar et al [17] have earlier obtained embryogenic callus and subsequent SE when hypocotyl, leaf and cotyledons of G. sylvestre seedlings were used as initial explants for establishment of its in vitro cultures. In the meristem culture and subsequent micropropagation of Chilean strawberry accessions, the use of BAP in the cultivation medium increased plantlets multiplication up to 3–6 micro shoots/explant [25] while it gave more number of shoots over Kin when amended in the culture medium of Senna occidentalis hypocotyl-derived callus cultures [12].…”

Section: Discussionmentioning

confidence: 99%

“…Previous in vitro clonal propagation of G. sylvestre were achieved from mature explants through direct regeneration pathways [6, 14–16] and somatic embryogenesis (SE) from seedling explants [17] but, totipotency expression potential of its cells via de novo organogenesis from meristematic tissues-derived callus cultures have not been studied. The de novo shoot morphogenic pathway is not attainable with many woody plants due to the influence of genotype and specific requirement for certain cells having unique properties needed for initiation of meristematic primordial essential for the in vitro morphogenesis [18, 19].…”

Section: Introductionmentioning

confidence: 99%

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De novo in vitro shoot morphogenesis from shoot tip-induced callus cultures of Gymnema sylvestre (Retz.) R.Br. ex Sm

Isah

2019

Biol Res

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BackgroundGymnema sylvestre is a medicinal woody perennial vine known for its sweetening properties and anti-diabetic therapeutic uses in the modern and traditional medicines. Its over-exploitation for the therapeutic uses and to meet the demand of pharmaceutical industry in raw materials supply for the production of anti-diabetic drugs has led to considerable decline in its natural population.ResultsAn efficient system of shoot bud sprouting from nodal segment explants and indirect plant regeneration from apical meristem-induced callus cultures of G. sylvestre have been developed on Murashige and Skoog (MS) medium amended with concentrations of cytokinins. Of the three growth regulators tested, N6-benzylaminopurine (BAP) was the most efficient and 2.0 mg L−1 gave the best shoot formation efficiency. This was followed by thidiazuron (TDZ) and kinetin (Kin) but, most of the TDZ-induced micro shoots showed stunted growth. Multiple shoot formation was observed on medium amended with BAP or TDZ at higher concentrations. The produced micro shoots were rooted on half strength MS medium amended with auxins and rooted plantlets acclimatized with 87% survival of the regenerates.ConclusionsThe developed regeneration system can be exploited for genetic transformation studies, particularly when aimed at producing its high yielding cell lines for the anti-diabetic phytochemicals. It also offers opportunities for exploring the expression of totipotency in the anti-diabetic perennial vine.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

De novo in vitro shoot morphogenesis from shoot tip-induced callus cultures of Gymnema sylvestre (Retz.) R.Br. ex Sm

Isah

2019

Biol Res

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“…In addition, there have been many reports of the efficacy of HSs, obtained from aseptic seedlings, to achieve indirect SE in many plant species [ 259 , 260 ]. Husain, Anis, and Shahzad [ 144 ] achieved SE from the callus, derived from hypocotyl segment (excised from12-day-old axenic seedlings) of P. marsupium .…”

Section: Propagation Of Pterocarpus Marsupiummentioning

confidence: 99%

Biotechnological Advances in Pharmacognosy and In Vitro Manipulation of Pterocarpus marsupium Roxb.

Ahmad

Anis

et al. 2022

Plants

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Trees are vital resources for economic, environmental, and industrial growth, supporting human life directly or indirectly through a wide variety of therapeutic compounds, commodities, and ecological services. Pterocarpus marsupium Roxb. (Fabaceae) is one of the most valuable multipurpose forest trees in India and Sri Lanka, as it is cultivated for quality wood as well as pharmaceutically bioactive compounds, especially from the stem bark and heartwood. However, propagation of the tree in natural conditions is difficult due to the low percentage of seed germination coupled with overexploitation of this species for its excellent multipurpose properties. This overexploitation has ultimately led to the inclusion of P. marsupium on the list of endangered plant species. However, recent developments in plant biotechnology may offer a solution to the overuse of such valuable species if such advances are accompanied by technology transfer in the developing world. Specifically, techniques in micropropagation, genetic manipulation, DNA barcoding, drug extraction, delivery, and targeting as well as standardization, are of substantial concern. To date, there are no comprehensive and detailed reviews of P. marsupium in terms of biotechnological research developments, specifically pharmacognosy, pharmacology, tissue culture, authentication of genuine species, and basic gene transfer studies. Thus, the present review attempts to present a comprehensive overview of the biotechnological studies centered on this species and some of the recent novel approaches for its genetic improvement.

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Extraction and quantification of gymnemic acids through gymnemagenin from callus cultures ofGymnema sylvestre

et al. 2006

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The phyto-constituents of Gymnema sylvestre are used in the treatment of diabetes and obesity. The present work reports on the extraction of gymnemic acid through gymnemagenin from callus cultures of G. sylvestre. Components were separated on pre-coated silica gel 60 GF254 plates with chloroform:methanol (8:2) and scanned using a densitometric scanner at 205 nm in the near-UV region. Linearity of determination of gymnemagenin was observed in the range 2-10 microg. The average percentage recovery of gymnemagenin from leaf callus extracts was 98.9+/-0.3.

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Cited by 30 publications

References 6 publications

De novo in vitro shoot morphogenesis from shoot tip-induced callus cultures of Gymnema sylvestre (Retz.) R.Br. ex Sm

De novo in vitro shoot morphogenesis from shoot tip-induced callus cultures of Gymnema sylvestre (Retz.) R.Br. ex Sm

Biotechnological Advances in Pharmacognosy and In Vitro Manipulation of Pterocarpus marsupium Roxb.

Extraction and quantification of gymnemic acids through gymnemagenin from callus cultures ofGymnema sylvestre

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