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Gaspar, Thomas; Franck, Thierry; Bisbis, Badia; Kevers, Claire; Jouve, Laurent; Hausman, Jean-François; Dommès, Jacques

doi:10.1023/a:1020835304842

Cited by 245 publications

(65 citation statements)

References 84 publications

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“…Our previous [2,3] and this study showed significant differences among Pelargonium genotypes in their susceptibility to leaf yellowing. Early senescence is widely influenced by stress conditions [18]. Plantlets cultivated in vitro are exposed to very stressful conditions due to wounding, abnormal mineral nutrition, unusual hormonal treatment, and high osmoticity [7,18].…”

Section: Discussionmentioning

confidence: 99%

“…Early senescence is widely influenced by stress conditions [18]. Plantlets cultivated in vitro are exposed to very stressful conditions due to wounding, abnormal mineral nutrition, unusual hormonal treatment, and high osmoticity [7,18]. For example, MS medium provides more than 60 mM of nitrogen, which exceeds about 80 times the level normally found in the soil [19].…”

Section: Discussionmentioning

confidence: 99%

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Effects of cytokinins on antioxidant enzymes in in vitro grown shoots of Pelargonium hortorum L. H. Bayley

Wojtania¹,

Skrzypek²

2014

Acta Agrobot

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A b s t r a c tThe aim of this study was to determine the influence of meta-topolin (mT) and 6-benzyl-aminopurine (BAP) on the hydrogen peroxide (H 2 O 2 ) level and antioxidant enzymes activities in relation to the shoot formation and senescence process in Pelargonium hortorum cultivars, which differ in their susceptibility to leaf yellowing under in vitro conditions.In an early senescing cultivar 'Grand Prix', the addition of an aromatic cytokinin mT to abscisic acid (ABA)-enriched Murashige and Skoog (MS) basal medium more efficiently inhibited leaf yellowing than BAP. In both genotypes, meta-topolin was also the most effective in shoot formation. It was found that Pelargonium species varying in their susceptibility to senescence differ in H 2 O 2 production and antioxidant enzymes activities. Generally, meta-topolin more effectively enhanced H 2 O 2 production and POD activity than BAP and control medium, but its effect depended on genotype. The highest H 2 O 2 production stimulated by mT was observed on day 5 of subculture in late senescing cv. 'Bergpalais'. In both geranium genotypes, superoxide dismutase (SOD) and catalase (CAT) levels were highest at the beginning of the subculture period, during the initiation of shoot formation. SOD showed the highest activity on day 5 of subculture on the medium without cytokinin and generally being higher in cv. 'Bergpalais' than in cv. 'Grand Prix'. CAT activity was positively regulated by both cytokinins. POD activity was most effectively enhanced by mT, but on different days of subculture -on the 2 nd day of subculture in cv. 'Bergpalais' and on the 22 nd day of subculture in cv. 'Grand Prix'. The enhanced activity of POD in the presence of mT, 4-fold higher than on control medium, at the end of subculture in P. hortorum 'Grand Prix' coincided with the inhibition of leaf senescence.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of cytokinins on antioxidant enzymes in in vitro grown shoots of Pelargonium hortorum L. H. Bayley

Wojtania¹,

Skrzypek²

2014

Acta Agrobot

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“…Thus, there are several reports related to proteins accumulation during maturation phase of somatic embryogenesis (Misra et al 1993), changes in their profiles (Roja Rani et al 2005) or to their function as somatic embryogenic receptors (Karlova et al 2006). On the other hand, proline accumulation is known to be involved in responses to saline and osmotic stresses (Lefèvre et al 2001;Gaspar et al 2002). Some studies focused on the relation between the content of proline and somatic embryogenesis have been reported (Murch et al 1999;Tonon et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Effect of exogenous arginine on sugarcane (Saccharum sp.) somatic embryogenesis, free polyamines and the contents of the soluble proteins and proline

Nieves

Sagarra

Gonzalez

et al. 2008

Plant Cell Tiss Organ Cult

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The aim of this study was to evaluate the effect of arginine on sugarcane (Saccharum sp.) somatic embryogenesis, free polyamines and other nitrogenous compounds contents. Segments of leaves were used as explants to establish embryogenic cultures on media with 0 and 50.0 mg l -1 arginine. Somatic embryos formation and free polyamines, free proline and total soluble proteins contents were compared. Arginine significantly induced sugarcane somatic embryogenesis. Free proline and protein levels determined in embryogenic cell masses during embryo differentiation-maturation, showed an arginine-induced promotion associated to the enhancement of the embryogenic process. In addition, free putrescine and, in a minor extent, spermidine and spermine contents were enhanced by arginine.

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“…As partes aéreas propagadas in vitro estão sob contínua condição de estresse (GASPAR et al, 2002;KEVERS et al, 2004) (PICOLI et al, 2001;GASPAR et al, 2002;ORCHATT et al, 2002). O acúmulo de ROS e a indução do estresse oxidativo foram observados na maioria dos tecidos hiperídricos descritos na literatura, entretanto, pouco é conhecido sobre a origem do estresse oxidativo induzido em tecidos hiperídricos (FERNANDEZ-GARCIA et al, 2011).…”

Section: Fatores Indutores Da Hiperidricidadeunclassified