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Appel, Heidi M.; Govenor, Heather L; D’Ascenzo, Mark; Siska, Erin L.; Schultz, Jack C.

doi:10.1023/a:1010306103643

Cited by 131 publications

(16 citation statements)

References 10 publications

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“…Total flavonoid compounds were also estimated in the same extracts at absorbance 320 nm (Fukumoto and Mazza 2000; Shao et al 2008). The concentration of total redox-reactive phenolics present in leaf extracts was determined using the Folin-Denis assay, with standard curves developed using chlorogenic and gallic acids, and standards purified from each treatment group (Appel et al 2001). …”

Section: Methodsmentioning

confidence: 99%

Plants respond to leaf vibrations caused by insect herbivore chewing

2014

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Plant germination and growth can be influenced by sound, but the ecological significance of these responses is unclear. We asked whether acoustic energy generated by the feeding of insect herbivores was detected by plants. We report that the vibrations caused by insect feeding can elicit chemical defenses. Arabidopsis thaliana (L.) rosettes pre-treated with the vibrations caused by caterpillar feeding had higher levels of glucosinolate and anthocyanin defenses when subsequently fed upon by Pieris rapae (L.) caterpillars than did untreated plants. The plants also discriminated between the vibrations caused by chewing and those caused by wind or insect song. Plants thus respond to herbivore-generated vibrations in a selective and ecologically meaningful way. A vibration signaling pathway would complement the known signaling pathways that rely on volatile, electrical, or phloem-borne signals. We suggest that vibration may represent a new long distance signaling mechanism in plant–insect interactions that contributes to systemic induction of chemical defenses.Electronic supplementary materialThe online version of this article (doi:10.1007/s00442-014-2995-6) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Plants respond to leaf vibrations caused by insect herbivore chewing

2014

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“…The crude fractions were purified and the components were isolated. The structures of the isolated components were determined using JMN-NMR 400 MHz and HPLC (Instrument: HITACHI HPLC D-7000 series, column: Cosmosil 5C18-AR-II Waters 4.6ϫ150 mm, mobile 14) and the content was calculated as the (ϩ)-catechin equivalent in the extract. Amino acid analysis was performed using by model L-8500A Hitachi high-speed amino acid analyzer.…”

Section: Methodsmentioning

confidence: 99%

Scavenging of Reactive Oxygen Species by Eriobotrya japonica Seed Extract

Yokota

Takuma

Hamada

et al. 2006

Biological & Pharmaceutical Bulletin

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We have clarified that Eriobotrya japonica seed extract has strong antioxidative activity, and is effective for the prevention and treatment of various diseases, such as hepatopathy and nephropathy. In this study, to investigate the influences of components of Eriobotrya japonica seed extract on its antioxidative activity, extracts were prepared using various solvents (n-hexane (Hex), ethyl acetate (EtOAc), n-butanol (n-BuOH), methanol (MeOH) and H 2 O) and the antioxidative activity of the solvent fractions and components was evaluated based on the scavenging of various radicals (DPPH and O 2 ؊ ) measured by the ESR method and the inhibition of Fe 3؉ -ADP induced NADPH dependent lipid peroxidation in rat liver microsomes. The radical scavenging activities and inhibitory activities on lipid peroxidation differed among the solvent fractions and components. In the n-BuOH, MeOH and H 2 O fractions, radical scavenging activity and inhibitory activity on lipid peroxidation were high. In addition, these fractions contained abundant polyphenols, and the radical scavenging activity increased with the polyphenol content. In the low-polar Hex and EtOAc fractions, the radical scavenging activity was low, but the lipid peroxidation inhibition activity was high. These fractions contained b b-sitosterol, and the inhibitory activity on lipid peroxidation was high. Based on these findings, the antioxidative activity of Eriobotrya japonica seed extract may be derived from many components involved in a complex mechanism, resulting in high activity.

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“…Many chemically similar compounds interfere with the test results. The selection of standards for calibration is critical because the reduction of F-C reagent by the standard and the phenolics in sample are comparable only if they are chemically very close [23]. Different standards such as gallic acid, ferulic acid, chlorogenic acid, vanillic acid, and catechol are being used by researchers, which generate different calibration curves and results in different numeric values of phenolics content of the same test sample [24].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Standards and Interfering Compounds in the Determination of Phenolics by Folin-Ciocalteu Assay Method for Effective Bioprocessing of Biomass

Bastola¹,

Guragain²,

Bhadriraju³

et al. 2017

AJAC

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Folin Ciocalteu (F-C) assay is the most widely used and convenient method to determine the total phenolics content in foods, herbs, and other plant extracts. Different phenolics standards such as gallic acid, ferulic acid, chlorogenic acid, catechol, and vanillic acid have been used for calibration curves in this assay method. Comparison of these standards, in single or combination of two or more, for more accurate determination of phenolics has not been reported so far. This study tested five single phenolics and seven combinations of mixed phenolic standards to evaluate the optimal standards for F-C method. The different standards were tested to calculate the phenolic content in three known test solutions. We also evaluated interference effect of various compounds in phenolics estimation by F-C method that is usually present in the lignocellulosic biomass-derived sugar solution, and in food products along with phenolics. Finally, the optimal standards with five phenolics were used for the determination of phenolics in alkali pretreated biomass extract. The results indicated that gallic acid was the best standard among the single phenolic compounds and five phenolic compounds solution was the best standard among the mixed phenolic solutions. The presence of glucose, HMF, furfural, and vitamin-B12 did not interfere in phenolic determination; whereas ascorbic acid, tyrosine, formic acid, and acetic acid strongly interfered the results. The results also showed that biomass pelleting process did not affect the generation of phenolics in alkali pretreatment extract.

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Untitled

Cited by 131 publications

References 10 publications

Plants respond to leaf vibrations caused by insect herbivore chewing

Plants respond to leaf vibrations caused by insect herbivore chewing

Scavenging of Reactive Oxygen Species by Eriobotrya japonica Seed Extract

Evaluation of Standards and Interfering Compounds in the Determination of Phenolics by Folin-Ciocalteu Assay Method for Effective Bioprocessing of Biomass

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